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Article: Expression of Brassica juncea 3-hydroxy-3-methylglutaryl CoA synthase is developmentally regulated and stress-responsive
Title | Expression of Brassica juncea 3-hydroxy-3-methylglutaryl CoA synthase is developmentally regulated and stress-responsive |
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Authors | |
Issue Date | 2000 |
Publisher | Wiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7412 |
Citation | Plant Journal, 2000, v. 22 n. 5, p. 415-426 How to Cite? |
Abstract | 3-Hydroxy-3-methylglutaryl-coenzymeA synthase (HMGS) is an enzyme in mevalonate biosynthesis. In plants, investigations have focused on HMG CoA reductase (HMGR) and less is known of the preceding enzyme, HMGS. To understand the regulation of HMGS, we have isolated a Brassica juncea cDNA encoding HMGS, BjHMGS1, for use as a hybridization probe in Northern blot analyses. BjHMGS is expressed in all plant organs and shows developmental regulation in flower, seed and seedling, with highest expression in early development. In seedlings, expression is highest in young hypocotyls and is induced during the greening of etiolated cotyledons. BjHMGS is down-regulated by abscisic acid, osmotic stress and dehydration, the effects of which arrested seedling growth. Thus BjHMGS expression shows correlation with rapid cell division and growth, like HMGR. This is not unexpected, as mevalonate is the precursor to many essential isoprenoid compounds, including sterols for membrane biogenesis. Wounding, methyl jasmonate or salicylic acid induce BjHMGS expression, suggesting that, like HMGR, HMGS is involved in defence. As in animals, coordinated regulation of HMGS with HMGR occurred in B. juncea upon germination and in response to salicylic acid. HMGS assays confirmed that Escherichia coli-expressed recombinant BjHMGS1 shows HMGS activity that is inhibited by F244, a specific inhibitor of HMGS. Southern blot analysis revealed gene families encoding HMGS in Brassica species and a summation of homologous genes in the fusion amphidiploid genome of B. juncea, a bi-parental species derived from diploids B. nigra and B. campestris. |
Persistent Identifier | http://hdl.handle.net/10722/178711 |
ISSN | 2023 Impact Factor: 6.2 2023 SCImago Journal Rankings: 2.176 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Alex, D | en_US |
dc.contributor.author | Bach, TJ | en_US |
dc.contributor.author | Chye, ML | en_US |
dc.date.accessioned | 2012-12-19T09:49:17Z | - |
dc.date.available | 2012-12-19T09:49:17Z | - |
dc.date.issued | 2000 | en_US |
dc.identifier.citation | Plant Journal, 2000, v. 22 n. 5, p. 415-426 | en_US |
dc.identifier.issn | 0960-7412 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/178711 | - |
dc.description.abstract | 3-Hydroxy-3-methylglutaryl-coenzymeA synthase (HMGS) is an enzyme in mevalonate biosynthesis. In plants, investigations have focused on HMG CoA reductase (HMGR) and less is known of the preceding enzyme, HMGS. To understand the regulation of HMGS, we have isolated a Brassica juncea cDNA encoding HMGS, BjHMGS1, for use as a hybridization probe in Northern blot analyses. BjHMGS is expressed in all plant organs and shows developmental regulation in flower, seed and seedling, with highest expression in early development. In seedlings, expression is highest in young hypocotyls and is induced during the greening of etiolated cotyledons. BjHMGS is down-regulated by abscisic acid, osmotic stress and dehydration, the effects of which arrested seedling growth. Thus BjHMGS expression shows correlation with rapid cell division and growth, like HMGR. This is not unexpected, as mevalonate is the precursor to many essential isoprenoid compounds, including sterols for membrane biogenesis. Wounding, methyl jasmonate or salicylic acid induce BjHMGS expression, suggesting that, like HMGR, HMGS is involved in defence. As in animals, coordinated regulation of HMGS with HMGR occurred in B. juncea upon germination and in response to salicylic acid. HMGS assays confirmed that Escherichia coli-expressed recombinant BjHMGS1 shows HMGS activity that is inhibited by F244, a specific inhibitor of HMGS. Southern blot analysis revealed gene families encoding HMGS in Brassica species and a summation of homologous genes in the fusion amphidiploid genome of B. juncea, a bi-parental species derived from diploids B. nigra and B. campestris. | en_US |
dc.language | eng | en_US |
dc.publisher | Wiley-Blackwell Publishing Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0960-7412 | en_US |
dc.relation.ispartof | Plant Journal | en_US |
dc.rights | The Plant Journal. Copyright © Blackwell Publishing Ltd. | - |
dc.subject.mesh | Abscisic Acid - Pharmacology | en_US |
dc.subject.mesh | Amino Acid Sequence | en_US |
dc.subject.mesh | Base Sequence | en_US |
dc.subject.mesh | Brassica - Enzymology - Genetics - Growth & Development | en_US |
dc.subject.mesh | Dna Primers | en_US |
dc.subject.mesh | Dna, Complementary | en_US |
dc.subject.mesh | Down-Regulation | en_US |
dc.subject.mesh | Gene Expression Regulation, Developmental - Drug Effects | en_US |
dc.subject.mesh | Gene Expression Regulation, Enzymologic - Drug Effects | en_US |
dc.subject.mesh | Gene Expression Regulation, Plant - Drug Effects | en_US |
dc.subject.mesh | Hydroxymethylglutaryl-Coa Synthase - Chemistry - Genetics | en_US |
dc.subject.mesh | Mannitol - Pharmacology | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Multigene Family | en_US |
dc.subject.mesh | Recombinant Proteins - Chemistry - Genetics | en_US |
dc.subject.mesh | Sequence Homology, Amino Acid | en_US |
dc.title | Expression of Brassica juncea 3-hydroxy-3-methylglutaryl CoA synthase is developmentally regulated and stress-responsive | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chye, ML: mlchye@hkucc.hku.hk | en_US |
dc.identifier.authority | Chye, ML=rp00687 | en_US |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.doi | 10.1046/j.1365-313X.2000.00751.x | en_US |
dc.identifier.pmid | 10849357 | - |
dc.identifier.scopus | eid_2-s2.0-0034124423 | en_US |
dc.identifier.hkuros | 48812 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-0034124423&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 22 | en_US |
dc.identifier.issue | 5 | en_US |
dc.identifier.spage | 415 | en_US |
dc.identifier.epage | 426 | en_US |
dc.identifier.isi | WOS:000087312200004 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Alex, D=55255924500 | en_US |
dc.identifier.scopusauthorid | Bach, TJ=24786011400 | en_US |
dc.identifier.scopusauthorid | Chye, ML=7003905460 | en_US |
dc.identifier.issnl | 0960-7412 | - |