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- Publisher Website: 10.1016/j.ecoenv.2006.11.012
- Scopus: eid_2-s2.0-34848850407
- PMID: 17296224
- WOS: WOS:000251067500008
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Article: Metabolism and biochemical pathway of n-butyl benzyl phthalate by Pseudomonas fluorescens B-1 isolated from a mangrove sediment
Title | Metabolism and biochemical pathway of n-butyl benzyl phthalate by Pseudomonas fluorescens B-1 isolated from a mangrove sediment |
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Authors | |
Keywords | Biodegradation Endocrine-disruptor Kinetics n-Butyl benzyl phthalate Pseudomonas fluorescens |
Issue Date | 2007 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/ecoenv |
Citation | Ecotoxicology And Environmental Safety, 2007, v. 68 n. 3, p. 379-385 How to Cite? |
Abstract | n-Butyl benzyl phthalate (BBP) is an endocrine-disrupting chemical. Biodegradation of BBP was investigated using the bacterium Pseudomonas fluorescens B-1 isolated from mangrove sediment of Mai Po Nature Reserve of Hong Kong. The microorganism was capable of utilizing BBP as the sole source of carbon and energy while BBP was degraded in 6 days under aerobic batch culture conditions. The optimum pH, temperature, and salinity for BBP degradation by P. fluorescens B-1 was found to be 7.0, 37 °C, and 15‰, respectively. Biodegradation of BBP was fitted to the first-order kinetics model. The process of BBP biodegradation was monitored by reversed-phase high-performance liquid chromatography with ultra-violet detection after solid-phase extraction. The major metabolites of BBP degradation were identified as mono-butyl phthalate, mono-benzyl phthalate, phthalic acid, and benzoic acid by gas chromatography-mass spectrometry. BBP-degrading activity of P. fluorescens B-1 was found mostly in the soluble fraction associated with the smaller fragments of cellular membranes. Results suggest that mineralization of BBP can be achieved by microorganism of the mangrove environment. © 2006 Elsevier Inc. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/179013 |
ISSN | 2023 Impact Factor: 6.2 2023 SCImago Journal Rankings: 1.418 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Xu, XR | en_US |
dc.contributor.author | Li, HB | en_US |
dc.contributor.author | Gu, JD | en_US |
dc.date.accessioned | 2012-12-19T09:51:25Z | - |
dc.date.available | 2012-12-19T09:51:25Z | - |
dc.date.issued | 2007 | en_US |
dc.identifier.citation | Ecotoxicology And Environmental Safety, 2007, v. 68 n. 3, p. 379-385 | en_US |
dc.identifier.issn | 0147-6513 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179013 | - |
dc.description.abstract | n-Butyl benzyl phthalate (BBP) is an endocrine-disrupting chemical. Biodegradation of BBP was investigated using the bacterium Pseudomonas fluorescens B-1 isolated from mangrove sediment of Mai Po Nature Reserve of Hong Kong. The microorganism was capable of utilizing BBP as the sole source of carbon and energy while BBP was degraded in 6 days under aerobic batch culture conditions. The optimum pH, temperature, and salinity for BBP degradation by P. fluorescens B-1 was found to be 7.0, 37 °C, and 15‰, respectively. Biodegradation of BBP was fitted to the first-order kinetics model. The process of BBP biodegradation was monitored by reversed-phase high-performance liquid chromatography with ultra-violet detection after solid-phase extraction. The major metabolites of BBP degradation were identified as mono-butyl phthalate, mono-benzyl phthalate, phthalic acid, and benzoic acid by gas chromatography-mass spectrometry. BBP-degrading activity of P. fluorescens B-1 was found mostly in the soluble fraction associated with the smaller fragments of cellular membranes. Results suggest that mineralization of BBP can be achieved by microorganism of the mangrove environment. © 2006 Elsevier Inc. All rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/locate/ecoenv | en_US |
dc.relation.ispartof | Ecotoxicology and Environmental Safety | en_US |
dc.subject | Biodegradation | - |
dc.subject | Endocrine-disruptor | - |
dc.subject | Kinetics | - |
dc.subject | n-Butyl benzyl phthalate | - |
dc.subject | Pseudomonas fluorescens | - |
dc.subject.mesh | Benzoic Acids - Metabolism | en_US |
dc.subject.mesh | Biodegradation, Environmental | en_US |
dc.subject.mesh | Carbon - Metabolism | en_US |
dc.subject.mesh | Chromatography, High Pressure Liquid - Methods | en_US |
dc.subject.mesh | Endocrine Disruptors - Metabolism | en_US |
dc.subject.mesh | Energy Metabolism | en_US |
dc.subject.mesh | Gas Chromatography-Mass Spectrometry | en_US |
dc.subject.mesh | Geologic Sediments - Microbiology | en_US |
dc.subject.mesh | Hong Kong | en_US |
dc.subject.mesh | Hydrogen-Ion Concentration | en_US |
dc.subject.mesh | Kinetics | en_US |
dc.subject.mesh | Models, Biological | en_US |
dc.subject.mesh | Phthalic Acids - Metabolism | en_US |
dc.subject.mesh | Pseudomonas Fluorescens - Enzymology - Isolation & Purification - Metabolism | en_US |
dc.subject.mesh | Rhizophoraceae | en_US |
dc.subject.mesh | Sodium Chloride - Metabolism | en_US |
dc.subject.mesh | Soil Microbiology | en_US |
dc.subject.mesh | Soil Pollutants - Metabolism | en_US |
dc.subject.mesh | Temperature | en_US |
dc.subject.mesh | Wetlands | en_US |
dc.title | Metabolism and biochemical pathway of n-butyl benzyl phthalate by Pseudomonas fluorescens B-1 isolated from a mangrove sediment | en_US |
dc.type | Article | en_US |
dc.identifier.email | Gu, JD: jdgu@hkucc.hku.hk | en_US |
dc.identifier.authority | Gu, JD=rp00701 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.ecoenv.2006.11.012 | en_US |
dc.identifier.pmid | 17296224 | - |
dc.identifier.scopus | eid_2-s2.0-34848850407 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-34848850407&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 68 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 379 | en_US |
dc.identifier.epage | 385 | en_US |
dc.identifier.isi | WOS:000251067500008 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Xu, XR=7405293882 | en_US |
dc.identifier.scopusauthorid | Li, HB=25649944400 | en_US |
dc.identifier.scopusauthorid | Gu, JD=7403129601 | en_US |
dc.identifier.issnl | 0147-6513 | - |