File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/j.bbrc.2007.09.108
- Scopus: eid_2-s2.0-35348964146
- PMID: 17936249
- WOS: WOS:000250661500023
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Study of the hypoxia-dependent regulation of human CYGB gene
Title | Study of the hypoxia-dependent regulation of human CYGB gene |
---|---|
Authors | |
Keywords | Cytoglobin (CYGB) Hypoxia Hypoxia inducible factor (HIF-1) Hypoxia responsive elements (HREs) Normoxia |
Issue Date | 2007 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description |
Citation | Biochemical And Biophysical Research Communications, 2007, v. 364 n. 1, p. 145-150 How to Cite? |
Abstract | Cytoglobin (CYGB) is ubiquitously expressed in all tissues and has been characterized as a respiratory protein in connective tissues. CYGB is up-regulated during hypoxia, implicating its function in maintaining the homeostasis redox of the cell. Here, we study the underlying molecular mechanisms by which hypoxia regulates human CYGB gene expression. When cells were subjected to hypoxia, the expression of endogenous CYGB was up-regulated ∼1.7-fold in BEAS-2B cells (p ≤ 0.05) and ∼1.6-fold in HeLa cells (p ≤ 0.05). Dual-luciferase assays and site directed mutagenesis showed the presence of hypoxia responsive elements (HREs) at positions -141, -144 and -448 that were essential for activation of CYGB expression under hypoxic conditions. The binding of hypoxia inducible factor (HIF-1) protein to the HREs was confirmed by gel shift and chromatin immunoprecipitation (ChIP) assays. These results indicate that HRE motifs are directly involved in the activation of the CYGB transcription under hypoxia. © 2007 Elsevier Inc. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/179015 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.770 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Guo, X | en_US |
dc.contributor.author | Philipsen, S | en_US |
dc.contributor.author | TanUn, KC | en_US |
dc.date.accessioned | 2012-12-19T09:51:25Z | - |
dc.date.available | 2012-12-19T09:51:25Z | - |
dc.date.issued | 2007 | en_US |
dc.identifier.citation | Biochemical And Biophysical Research Communications, 2007, v. 364 n. 1, p. 145-150 | en_US |
dc.identifier.issn | 0006-291X | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179015 | - |
dc.description.abstract | Cytoglobin (CYGB) is ubiquitously expressed in all tissues and has been characterized as a respiratory protein in connective tissues. CYGB is up-regulated during hypoxia, implicating its function in maintaining the homeostasis redox of the cell. Here, we study the underlying molecular mechanisms by which hypoxia regulates human CYGB gene expression. When cells were subjected to hypoxia, the expression of endogenous CYGB was up-regulated ∼1.7-fold in BEAS-2B cells (p ≤ 0.05) and ∼1.6-fold in HeLa cells (p ≤ 0.05). Dual-luciferase assays and site directed mutagenesis showed the presence of hypoxia responsive elements (HREs) at positions -141, -144 and -448 that were essential for activation of CYGB expression under hypoxic conditions. The binding of hypoxia inducible factor (HIF-1) protein to the HREs was confirmed by gel shift and chromatin immunoprecipitation (ChIP) assays. These results indicate that HRE motifs are directly involved in the activation of the CYGB transcription under hypoxia. © 2007 Elsevier Inc. All rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description | en_US |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | en_US |
dc.subject | Cytoglobin (CYGB) | - |
dc.subject | Hypoxia | - |
dc.subject | Hypoxia inducible factor (HIF-1) | - |
dc.subject | Hypoxia responsive elements (HREs) | - |
dc.subject | Normoxia | - |
dc.subject.mesh | Anoxia - Physiopathology | en_US |
dc.subject.mesh | Cell Line | en_US |
dc.subject.mesh | Chromatin Immunoprecipitation | en_US |
dc.subject.mesh | Electrophoretic Mobility Shift Assay | en_US |
dc.subject.mesh | Gene Expression Regulation | en_US |
dc.subject.mesh | Globins - Genetics | en_US |
dc.subject.mesh | Hela Cells | en_US |
dc.subject.mesh | Humans | en_US |
dc.subject.mesh | Hypoxia-Inducible Factor 1 - Metabolism | en_US |
dc.subject.mesh | Mutagenesis, Site-Directed | en_US |
dc.subject.mesh | Promoter Regions, Genetic | en_US |
dc.subject.mesh | Up-Regulation | en_US |
dc.title | Study of the hypoxia-dependent regulation of human CYGB gene | en_US |
dc.type | Article | en_US |
dc.identifier.email | TanUn, KC: kctanun@hkucc.hku.hk | en_US |
dc.identifier.authority | TanUn, KC=rp00787 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.bbrc.2007.09.108 | en_US |
dc.identifier.pmid | 17936249 | - |
dc.identifier.scopus | eid_2-s2.0-35348964146 | en_US |
dc.identifier.hkuros | 139815 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-35348964146&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 364 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 145 | en_US |
dc.identifier.epage | 150 | en_US |
dc.identifier.isi | WOS:000250661500023 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Guo, X=36725898900 | en_US |
dc.identifier.scopusauthorid | Philipsen, S=7003745052 | en_US |
dc.identifier.scopusauthorid | TanUn, KC=6602914262 | en_US |
dc.identifier.issnl | 0006-291X | - |