File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1111/j.1472-765X.2008.02427.x
- Scopus: eid_2-s2.0-52449106756
- PMID: 19241518
- WOS: WOS:000259362300004
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Genetic characterization of Mortierella alpina by sequencing the 18S-28S ribosomal gene internal transcribed spacer region
Title | Genetic characterization of Mortierella alpina by sequencing the 18S-28S ribosomal gene internal transcribed spacer region |
---|---|
Authors | |
Keywords | ITS region Mortierella alpina Soil fungus Zygomycetes |
Issue Date | 2008 |
Citation | Letters In Applied Microbiology, 2008, v. 47 n. 4, p. 250-255 How to Cite? |
Abstract | Aims: The aim of this study was to establish a genetic background of Mortierella alpina, which is a cosmopolitan, soil-inhabiting Zygomycete that also has important biotechnology potential. Methods and Results: A total of 44 18S-28S ribosomal gene internal transcribed spacer (ITS1-5.8S rDNA-ITS2 DNA) regions of M. alpina from three diverse locations (Far East Asia, North America and West-Central Europe) were sequenced and investigated. The sequences between M. alpina and the three closely related species (Mortierella macrocystis, Mortierella gamsii and Mortierella humilis) showed 74-84% sequence identity. When a phylogenetic tree was constructed with a neighbour-joining algorithm, four clades of M. alpina isolates were clearly distinct with high bootstrap values. In addition, isolates from the West-Central Europe were found to have the highest gene and nucleotide diversities. Conclusions: The ITS region was a suitable tool for distinguishing M. alpina from other closely related species. The region also provided information of the diversity of M. alpina. Significance and Impact of the Study: The study established a genetic background of M. alpina for identification and the diversity of M. alpina provided information for further isolation and screening of the fungus. © 2008 The Authors. |
Persistent Identifier | http://hdl.handle.net/10722/179085 |
ISSN | 2023 Impact Factor: 2.0 2023 SCImago Journal Rankings: 0.525 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ho, SY | en_US |
dc.contributor.author | Chen, F | en_US |
dc.date.accessioned | 2012-12-19T09:51:51Z | - |
dc.date.available | 2012-12-19T09:51:51Z | - |
dc.date.issued | 2008 | en_US |
dc.identifier.citation | Letters In Applied Microbiology, 2008, v. 47 n. 4, p. 250-255 | en_US |
dc.identifier.issn | 0266-8254 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179085 | - |
dc.description.abstract | Aims: The aim of this study was to establish a genetic background of Mortierella alpina, which is a cosmopolitan, soil-inhabiting Zygomycete that also has important biotechnology potential. Methods and Results: A total of 44 18S-28S ribosomal gene internal transcribed spacer (ITS1-5.8S rDNA-ITS2 DNA) regions of M. alpina from three diverse locations (Far East Asia, North America and West-Central Europe) were sequenced and investigated. The sequences between M. alpina and the three closely related species (Mortierella macrocystis, Mortierella gamsii and Mortierella humilis) showed 74-84% sequence identity. When a phylogenetic tree was constructed with a neighbour-joining algorithm, four clades of M. alpina isolates were clearly distinct with high bootstrap values. In addition, isolates from the West-Central Europe were found to have the highest gene and nucleotide diversities. Conclusions: The ITS region was a suitable tool for distinguishing M. alpina from other closely related species. The region also provided information of the diversity of M. alpina. Significance and Impact of the Study: The study established a genetic background of M. alpina for identification and the diversity of M. alpina provided information for further isolation and screening of the fungus. © 2008 The Authors. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Letters in Applied Microbiology | en_US |
dc.subject | ITS region | - |
dc.subject | Mortierella alpina | - |
dc.subject | Soil fungus | - |
dc.subject | Zygomycetes | - |
dc.subject.mesh | Dna, Ribosomal Spacer - Genetics | en_US |
dc.subject.mesh | Genetic Variation | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Mortierella - Genetics | en_US |
dc.subject.mesh | Phylogeny | en_US |
dc.title | Genetic characterization of Mortierella alpina by sequencing the 18S-28S ribosomal gene internal transcribed spacer region | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1111/j.1472-765X.2008.02427.x | en_US |
dc.identifier.pmid | 19241518 | en_US |
dc.identifier.scopus | eid_2-s2.0-52449106756 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-52449106756&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 47 | en_US |
dc.identifier.issue | 4 | en_US |
dc.identifier.spage | 250 | en_US |
dc.identifier.epage | 255 | en_US |
dc.identifier.isi | WOS:000259362300004 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Ho, SY=23980055900 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.citeulike | 3317341 | - |
dc.identifier.issnl | 0266-8254 | - |