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- Publisher Website: 10.1016/j.carres.2011.03.001
- Scopus: eid_2-s2.0-79955932541
- PMID: 21507388
- WOS: WOS:000291417000005
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Article: Structures of clusters in sweetpotato amylopectin
Title | Structures of clusters in sweetpotato amylopectin |
---|---|
Authors | |
Keywords | Bacillus amyloliquefaciens α-amylase C-type crystalline polymorph starch Cluster structure Sweetpotato amylopectin |
Issue Date | 2011 |
Publisher | Pergamon. The Journal's web site is located at http://www.elsevier.com/locate/carres |
Citation | Carbohydrate Research, 2011, v. 346 n. 9, p. 1112-1121 How to Cite? |
Abstract | Sweetpotato amylopectin was subjected to partial hydrolysis by α-amylase from Bacillus amyloliquefaciens to release the clusters. Clusters were then fractionated and precipitated by methanol and structurally characterized by gel-permeation chromatography and high-performance anion-exchange chromatography. An initial stage of α-amylolysis on the amylopectin isolated mostly domains but also clusters. A second stage of α-amylolysis on the domains and clusters further isolated their respective clusters and sub-clusters. All the domains, clusters and sub-clusters were sequentially subjected to phosphorolysis and β-amylolysis to obtain their internal part. The degree of polymerization of the clusters in the form of φ,β-limit dextrins were from 58 to 86. Each domain contained 2-8 clusters. Two types of clusters were structurally identified. Type A clusters were larger and contained about 12 chains per cluster with higher degree of branching (DB), whereas those of type B were smaller and contained about eight chains per cluster with lower DB. © 2011 Elsevier Ltd. All rights reserved. |
Persistent Identifier | http://hdl.handle.net/10722/179233 |
ISSN | 2023 Impact Factor: 2.4 2023 SCImago Journal Rankings: 0.509 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Zhu, F | en_US |
dc.contributor.author | Corke, H | en_US |
dc.contributor.author | Åman, P | en_US |
dc.contributor.author | Bertoft, E | en_US |
dc.date.accessioned | 2012-12-19T09:53:17Z | - |
dc.date.available | 2012-12-19T09:53:17Z | - |
dc.date.issued | 2011 | en_US |
dc.identifier.citation | Carbohydrate Research, 2011, v. 346 n. 9, p. 1112-1121 | en_US |
dc.identifier.issn | 0008-6215 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179233 | - |
dc.description.abstract | Sweetpotato amylopectin was subjected to partial hydrolysis by α-amylase from Bacillus amyloliquefaciens to release the clusters. Clusters were then fractionated and precipitated by methanol and structurally characterized by gel-permeation chromatography and high-performance anion-exchange chromatography. An initial stage of α-amylolysis on the amylopectin isolated mostly domains but also clusters. A second stage of α-amylolysis on the domains and clusters further isolated their respective clusters and sub-clusters. All the domains, clusters and sub-clusters were sequentially subjected to phosphorolysis and β-amylolysis to obtain their internal part. The degree of polymerization of the clusters in the form of φ,β-limit dextrins were from 58 to 86. Each domain contained 2-8 clusters. Two types of clusters were structurally identified. Type A clusters were larger and contained about 12 chains per cluster with higher degree of branching (DB), whereas those of type B were smaller and contained about eight chains per cluster with lower DB. © 2011 Elsevier Ltd. All rights reserved. | en_US |
dc.language | eng | en_US |
dc.publisher | Pergamon. The Journal's web site is located at http://www.elsevier.com/locate/carres | en_US |
dc.relation.ispartof | Carbohydrate Research | en_US |
dc.subject | Bacillus amyloliquefaciens α-amylase | - |
dc.subject | C-type crystalline polymorph starch | - |
dc.subject | Cluster structure | - |
dc.subject | Sweetpotato amylopectin | - |
dc.subject.mesh | Amylopectin - Chemistry | en_US |
dc.subject.mesh | Carbohydrate Conformation | en_US |
dc.subject.mesh | Chemical Fractionation | en_US |
dc.subject.mesh | Ipomoea Batatas - Chemistry | en_US |
dc.title | Structures of clusters in sweetpotato amylopectin | en_US |
dc.type | Article | en_US |
dc.identifier.email | Corke, H: harold@hku.hk | en_US |
dc.identifier.authority | Corke, H=rp00688 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1016/j.carres.2011.03.001 | en_US |
dc.identifier.pmid | 21507388 | - |
dc.identifier.scopus | eid_2-s2.0-79955932541 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-79955932541&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 346 | en_US |
dc.identifier.issue | 9 | en_US |
dc.identifier.spage | 1112 | en_US |
dc.identifier.epage | 1121 | en_US |
dc.identifier.isi | WOS:000291417000005 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Zhu, F=35306203800 | en_US |
dc.identifier.scopusauthorid | Corke, H=7007102942 | en_US |
dc.identifier.scopusauthorid | Åman, P=7004451997 | en_US |
dc.identifier.scopusauthorid | Bertoft, E=6604042426 | en_US |
dc.identifier.citeulike | 8987785 | - |
dc.identifier.issnl | 0008-6215 | - |