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- Publisher Website: 10.1099/ijs.0.02690-0
- Scopus: eid_2-s2.0-9744242714
- PMID: 15545446
- WOS: WOS:000225366000039
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Article: Klebsiella singaporensis sp. nov., a novel isomaltulose-producing bacterium
Title | Klebsiella singaporensis sp. nov., a novel isomaltulose-producing bacterium |
---|---|
Authors | |
Issue Date | 2004 |
Publisher | Society for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.org |
Citation | International Journal Of Systematic And Evolutionary Microbiology, 2004, v. 54 n. 6, p. 2131-2136 How to Cite? |
Abstract | Cells of strain LX3T, isolated from soil, were Gram-negative, facultatively anaerobic, non-motile, capsulated and non-endospore-forming straight rods, able to grow at 10 °C, unable to produce gas from lactose at 45 °C and unable to produce indole. The isolate converted sucrose to isomaltulose and did not produce detectable glucose by-products. The G+C content of the DNA was 56.4 mol%. Furthermore, comparison of 16S rRNA and rpoB gene sequences showed that the isolate clearly belongs to the genus Klebsiella. The closest phylogenetic relative was Kiebsiella pneumoniae, there being 99.3 and 97.5% similarity in 16S rRNA and rpoB gene sequences, respectively. DNA-DNA hybridization analysis demonstrated a very low level of relatedness to other members of the genus Klebsiella, indicating that the isolated strain and other species in the genus Kiebsiella were not related at the species level. The isolate could be differentiated from other previously described members of the genus Klebsiella on the basis of phenotypic differences and 16S rRNA and rpoB gene sequence divergence, together with DNA-DNA reassociation data. Therefore, it is proposed that strain LX3T (=DSM 16265T =JCM 12419T) should be classified as the type strain of a novel species of genus Klebsiella, Klebsiella singaporensis sp. nov. © 2004 IUMS. |
Persistent Identifier | http://hdl.handle.net/10722/179343 |
ISSN | 2023 Impact Factor: 2.0 2023 SCImago Journal Rankings: 0.952 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, X | en_US |
dc.contributor.author | Zhang, D | en_US |
dc.contributor.author | Chen, F | en_US |
dc.contributor.author | Ma, J | en_US |
dc.contributor.author | Dong, Y | en_US |
dc.contributor.author | Zhang, L | en_US |
dc.date.accessioned | 2012-12-19T09:54:20Z | - |
dc.date.available | 2012-12-19T09:54:20Z | - |
dc.date.issued | 2004 | en_US |
dc.identifier.citation | International Journal Of Systematic And Evolutionary Microbiology, 2004, v. 54 n. 6, p. 2131-2136 | en_US |
dc.identifier.issn | 1466-5026 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/179343 | - |
dc.description.abstract | Cells of strain LX3T, isolated from soil, were Gram-negative, facultatively anaerobic, non-motile, capsulated and non-endospore-forming straight rods, able to grow at 10 °C, unable to produce gas from lactose at 45 °C and unable to produce indole. The isolate converted sucrose to isomaltulose and did not produce detectable glucose by-products. The G+C content of the DNA was 56.4 mol%. Furthermore, comparison of 16S rRNA and rpoB gene sequences showed that the isolate clearly belongs to the genus Klebsiella. The closest phylogenetic relative was Kiebsiella pneumoniae, there being 99.3 and 97.5% similarity in 16S rRNA and rpoB gene sequences, respectively. DNA-DNA hybridization analysis demonstrated a very low level of relatedness to other members of the genus Klebsiella, indicating that the isolated strain and other species in the genus Kiebsiella were not related at the species level. The isolate could be differentiated from other previously described members of the genus Klebsiella on the basis of phenotypic differences and 16S rRNA and rpoB gene sequence divergence, together with DNA-DNA reassociation data. Therefore, it is proposed that strain LX3T (=DSM 16265T =JCM 12419T) should be classified as the type strain of a novel species of genus Klebsiella, Klebsiella singaporensis sp. nov. © 2004 IUMS. | en_US |
dc.language | eng | en_US |
dc.publisher | Society for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.org | en_US |
dc.relation.ispartof | International Journal of Systematic and Evolutionary Microbiology | en_US |
dc.subject.mesh | Anaerobiosis | en_US |
dc.subject.mesh | Bacterial Capsules - Biosynthesis | en_US |
dc.subject.mesh | Base Composition | en_US |
dc.subject.mesh | Dna, Bacterial - Chemistry - Isolation & Purification | en_US |
dc.subject.mesh | Dna, Ribosomal - Chemistry - Isolation & Purification | en_US |
dc.subject.mesh | Dna-Directed Rna Polymerases - Genetics | en_US |
dc.subject.mesh | Genes, Rrna | en_US |
dc.subject.mesh | Gentian Violet | en_US |
dc.subject.mesh | Glucose - Metabolism | en_US |
dc.subject.mesh | Indoles - Metabolism | en_US |
dc.subject.mesh | Isomaltose - Analogs & Derivatives - Biosynthesis | en_US |
dc.subject.mesh | Klebsiella - Classification - Cytology - Isolation & Purification - Physiology | en_US |
dc.subject.mesh | Lactose - Metabolism | en_US |
dc.subject.mesh | Molecular Sequence Data | en_US |
dc.subject.mesh | Movement | en_US |
dc.subject.mesh | Nucleic Acid Hybridization | en_US |
dc.subject.mesh | Phenazines | en_US |
dc.subject.mesh | Phylogeny | en_US |
dc.subject.mesh | Rna, Bacterial - Genetics | en_US |
dc.subject.mesh | Rna, Ribosomal, 16S - Genetics | en_US |
dc.subject.mesh | Sequence Analysis, Dna | en_US |
dc.subject.mesh | Soil Microbiology | en_US |
dc.subject.mesh | Spores, Bacterial - Cytology | en_US |
dc.subject.mesh | Sucrose - Metabolism | en_US |
dc.subject.mesh | Temperature | en_US |
dc.title | Klebsiella singaporensis sp. nov., a novel isomaltulose-producing bacterium | en_US |
dc.type | Article | en_US |
dc.identifier.email | Chen, F: sfchen@hku.hk | en_US |
dc.identifier.authority | Chen, F=rp00672 | en_US |
dc.description.nature | link_to_subscribed_fulltext | en_US |
dc.identifier.doi | 10.1099/ijs.0.02690-0 | en_US |
dc.identifier.pmid | 15545446 | - |
dc.identifier.scopus | eid_2-s2.0-9744242714 | en_US |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-9744242714&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 54 | en_US |
dc.identifier.issue | 6 | en_US |
dc.identifier.spage | 2131 | en_US |
dc.identifier.epage | 2136 | en_US |
dc.identifier.isi | WOS:000225366000039 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.scopusauthorid | Li, X=8869956800 | en_US |
dc.identifier.scopusauthorid | Zhang, D=7405355717 | en_US |
dc.identifier.scopusauthorid | Chen, F=7404907980 | en_US |
dc.identifier.scopusauthorid | Ma, J=7406200410 | en_US |
dc.identifier.scopusauthorid | Dong, Y=7403390435 | en_US |
dc.identifier.scopusauthorid | Zhang, L=8599921900 | en_US |
dc.identifier.issnl | 1466-5026 | - |