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- Publisher Website: 10.1039/c3mt00224a
- Scopus: eid_2-s2.0-84890953655
- PMID: 24201382
- WOS: WOS:000328891100005
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Article: In-cell NMR: an emerging approach for monitoring metal-related events in living cells
Title | In-cell NMR: an emerging approach for monitoring metal-related events in living cells |
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Authors | |
Issue Date | 2014 |
Publisher | Royal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/Publishing/Journals/MT/About.asp |
Citation | Metallomics, 2014, v. 6 n. 1, p. 69-76 How to Cite? |
Abstract | In-cell NMR, an isotope-assisted multi-dimensional NMR technique, has been proven to be successful in the investigation of protein dynamics, folding, conformational changes induced by binding events, posttranslational modification in the complex native environments, as well as in vivo drug screening, even de novo 3D protein structure determination in living cells. This technique was initially applied to bacterial cells, and subsequently has been extended to various other cells including eukaryotic cells. In this review, we briefly summarize the methodology and application of in-cell NMR with a focus on its application in metallomics and metalloproteomics. This emerging technique is anticipated to be an excellent tool for studying metal-associated events in complex native environments of living cells. |
Persistent Identifier | http://hdl.handle.net/10722/194673 |
ISSN | 2023 Impact Factor: 2.9 2023 SCImago Journal Rankings: 0.706 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Li, H | en_US |
dc.contributor.author | Sun, H | en_US |
dc.date.accessioned | 2014-02-17T02:02:41Z | - |
dc.date.available | 2014-02-17T02:02:41Z | - |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | Metallomics, 2014, v. 6 n. 1, p. 69-76 | en_US |
dc.identifier.issn | 1756-5901 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/194673 | - |
dc.description.abstract | In-cell NMR, an isotope-assisted multi-dimensional NMR technique, has been proven to be successful in the investigation of protein dynamics, folding, conformational changes induced by binding events, posttranslational modification in the complex native environments, as well as in vivo drug screening, even de novo 3D protein structure determination in living cells. This technique was initially applied to bacterial cells, and subsequently has been extended to various other cells including eukaryotic cells. In this review, we briefly summarize the methodology and application of in-cell NMR with a focus on its application in metallomics and metalloproteomics. This emerging technique is anticipated to be an excellent tool for studying metal-associated events in complex native environments of living cells. | en_US |
dc.language | eng | en_US |
dc.publisher | Royal Society of Chemistry. The Journal's web site is located at http://www.rsc.org/Publishing/Journals/MT/About.asp | en_US |
dc.relation.ispartof | Metallomics | en_US |
dc.title | In-cell NMR: an emerging approach for monitoring metal-related events in living cells | en_US |
dc.type | Article | en_US |
dc.identifier.email | Li, H: hylichem@hku.hk | en_US |
dc.identifier.email | Sun, H: hsun@hku.hk | en_US |
dc.identifier.authority | Sun, H=rp00777 | en_US |
dc.identifier.doi | 10.1039/c3mt00224a | - |
dc.identifier.pmid | 24201382 | - |
dc.identifier.scopus | eid_2-s2.0-84890953655 | - |
dc.identifier.hkuros | 227914 | en_US |
dc.identifier.volume | 6 | en_US |
dc.identifier.issue | 1 | en_US |
dc.identifier.spage | 69 | en_US |
dc.identifier.epage | 76 | en_US |
dc.identifier.isi | WOS:000328891100005 | - |
dc.publisher.place | United Kingdom | en_US |
dc.identifier.issnl | 1756-5901 | - |