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- Publisher Website: 10.1016/0014-5793(94)01457-C
- Scopus: eid_2-s2.0-0028942170
- PMID: 7531161
- WOS: WOS:A1995QE77400019
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Article: Intercellular channels in teleosts: functional characterization of two connexins from Atlantic croaker
Title | Intercellular channels in teleosts: functional characterization of two connexins from Atlantic croaker |
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Authors | |
Keywords | Gap junction Intercellular communication Oocyte Voltage Xenopus |
Issue Date | 1995 |
Publisher | Elsevier BV. |
Citation | FEBS Letters, 1995, v. 358 n. 3, p. 301-304 How to Cite? |
Abstract | Gap junction channels, composed of protein subunits termed connexins, are believed to play a critical role in the process of oocyte differentiation and maturation. We have used the paired Xenopus oocyte assay to characterize functionally two connexin genes, connexin-32.2 and connexin-32.7, recently cloned from the ovary of the Atlantic croaker (Micropogonia undulatus), a species that has emerged as a useful model to study the process of maturation of the ovarian follicle. We have found that, while both connexin proteins were expressed at comparable levels in Xenopus oocytes, only one, connexin-32.2, was functionally competent to induce the formation of intercellular channels. Connexin-32.2 channels exhibited voltage-dependent closure that was similar to, but distinct from that of previously characterized mammalian connexins. In addition, the silent connexin-32.7 was unable to functionally interact with connexin-32.2, either in heterotypic channels or as dominant negative inhibitor. Because connexin-32.2 expression is strikingly regulated during oocyte maturation, these data provide further evidence for a role of intercellular channels in the control of oocyte-follicular cell interactions. |
Persistent Identifier | http://hdl.handle.net/10722/195295 |
ISSN | 2023 Impact Factor: 3.0 2023 SCImago Journal Rankings: 1.208 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Bruzzone, R | - |
dc.contributor.author | White, TW | - |
dc.contributor.author | Yoshizaki, G | - |
dc.contributor.author | Patiño, R | - |
dc.contributor.author | Paul, DL | - |
dc.date.accessioned | 2014-02-26T06:21:48Z | - |
dc.date.available | 2014-02-26T06:21:48Z | - |
dc.date.issued | 1995 | - |
dc.identifier.citation | FEBS Letters, 1995, v. 358 n. 3, p. 301-304 | - |
dc.identifier.issn | 0014-5793 | - |
dc.identifier.uri | http://hdl.handle.net/10722/195295 | - |
dc.description.abstract | Gap junction channels, composed of protein subunits termed connexins, are believed to play a critical role in the process of oocyte differentiation and maturation. We have used the paired Xenopus oocyte assay to characterize functionally two connexin genes, connexin-32.2 and connexin-32.7, recently cloned from the ovary of the Atlantic croaker (Micropogonia undulatus), a species that has emerged as a useful model to study the process of maturation of the ovarian follicle. We have found that, while both connexin proteins were expressed at comparable levels in Xenopus oocytes, only one, connexin-32.2, was functionally competent to induce the formation of intercellular channels. Connexin-32.2 channels exhibited voltage-dependent closure that was similar to, but distinct from that of previously characterized mammalian connexins. In addition, the silent connexin-32.7 was unable to functionally interact with connexin-32.2, either in heterotypic channels or as dominant negative inhibitor. Because connexin-32.2 expression is strikingly regulated during oocyte maturation, these data provide further evidence for a role of intercellular channels in the control of oocyte-follicular cell interactions. | - |
dc.language | eng | - |
dc.publisher | Elsevier BV. | - |
dc.relation.ispartof | FEBS Letters | - |
dc.rights | NOTICE: this is the author’s version of a work that was accepted for publication in FEBS Letters. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in FEBS Letters, [VOL 358, ISSUE 3, 1995] DOI 10.1016/0014-5793(94)01457-C | - |
dc.subject | Gap junction | - |
dc.subject | Intercellular communication | - |
dc.subject | Oocyte | - |
dc.subject | Voltage | - |
dc.subject | Xenopus | - |
dc.subject.mesh | Connexins - genetics - metabolism - physiology | - |
dc.subject.mesh | Ion Channels - genetics - metabolism - physiology | - |
dc.subject.mesh | Molecular Sequence Data | - |
dc.subject.mesh | Perciformes - metabolism | - |
dc.subject.mesh | Xenopus laevis | - |
dc.title | Intercellular channels in teleosts: functional characterization of two connexins from Atlantic croaker | en_US |
dc.type | Article | en_US |
dc.identifier.email | Bruzzone, R: bruzzone@HKUCC.hku.hk | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/0014-5793(94)01457-C | - |
dc.identifier.pmid | 7531161 | - |
dc.identifier.scopus | eid_2-s2.0-0028942170 | - |
dc.identifier.volume | 358 | - |
dc.identifier.issue | 3 | - |
dc.identifier.spage | 301 | - |
dc.identifier.epage | 304 | - |
dc.identifier.isi | WOS:A1995QE77400019 | - |
dc.publisher.place | Netherlands | - |
dc.identifier.issnl | 0014-5793 | - |