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Conference Paper: In-vitro agarose hydrogel system for biomimetic mineralisation on demineralised dentine
Title | In-vitro agarose hydrogel system for biomimetic mineralisation on demineralised dentine |
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Authors | |
Keywords | Collagen Dentin Enamel Regeneration and Remineralization |
Issue Date | 2014 |
Publisher | Sage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925 |
Citation | The 92nd General Session & Exhibition of the International Association for Dental Research (IADR), Cape Town, South Africa, 25-28 June 2014. In Journal of Dental Research, 2014, v. 93 n. Special issue B: abstract no. 1207 How to Cite? |
Abstract | Objective: To develop an in vitro agarose hydrogel system for biomimetic mineralisation on demineralised dentine.
Method: Human dentine slices were prepared from extracted sound human molars. They were polished and acid-etched with 37% phosphoric acid for 15 sec to create demineralized dentine surfaces. The slices were put into the polyethylene tubes with the demineralized dentine surface covered by a 2-mm-thick layer of calcium chloride (CaCl2) agarose hydrogel. Another 2-mm-thick layer of ion-free agarose hydrogel was added on top of the CaCl2 agarose hydrogel. They were immersed into a freshly prepared solution containing phosphate and fluoride and incubated at 37˚C for 6 days. The solution was replaced every 24 hours and the agarose hydrogels were replaced every 48 hours. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) were used to evaluate the crystals formed on dentine surface. Nanoscratch test was carried out using a nano-indentor to evaluate the tribological property of the regenerated crystals with dentine.
Result: Observation under SEM showed that the crystals occluded the dentinal tubules and an enamel prism-like tissue formed on the demineralized dentine surface. XRD and FTIR analyses suggested that the crystals were hydroxyapatite. No crack was observed in the nanoscratch test.
Conclusion: In this study, an in vitro agarose hydrogel system was successfully developed to regenerate enamel prism-like tissue on demineralised dentine surface. |
Description | Poster Presentation Session 164: Emerging Knowledge on Pathogenesis and Management of Dental Caries |
Persistent Identifier | http://hdl.handle.net/10722/199335 |
ISSN | 2023 Impact Factor: 5.7 2023 SCImago Journal Rankings: 1.909 |
DC Field | Value | Language |
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dc.contributor.author | Cao, Y | en_US |
dc.contributor.author | Li, QL | en_US |
dc.contributor.author | Lo, ECM | en_US |
dc.contributor.author | Chu, CH | en_US |
dc.date.accessioned | 2014-07-22T01:13:40Z | - |
dc.date.available | 2014-07-22T01:13:40Z | - |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | The 92nd General Session & Exhibition of the International Association for Dental Research (IADR), Cape Town, South Africa, 25-28 June 2014. In Journal of Dental Research, 2014, v. 93 n. Special issue B: abstract no. 1207 | en_US |
dc.identifier.issn | 0022-0345 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/199335 | - |
dc.description | Poster Presentation | - |
dc.description | Session 164: Emerging Knowledge on Pathogenesis and Management of Dental Caries | - |
dc.description.abstract | Objective: To develop an in vitro agarose hydrogel system for biomimetic mineralisation on demineralised dentine. Method: Human dentine slices were prepared from extracted sound human molars. They were polished and acid-etched with 37% phosphoric acid for 15 sec to create demineralized dentine surfaces. The slices were put into the polyethylene tubes with the demineralized dentine surface covered by a 2-mm-thick layer of calcium chloride (CaCl2) agarose hydrogel. Another 2-mm-thick layer of ion-free agarose hydrogel was added on top of the CaCl2 agarose hydrogel. They were immersed into a freshly prepared solution containing phosphate and fluoride and incubated at 37˚C for 6 days. The solution was replaced every 24 hours and the agarose hydrogels were replaced every 48 hours. Scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR) were used to evaluate the crystals formed on dentine surface. Nanoscratch test was carried out using a nano-indentor to evaluate the tribological property of the regenerated crystals with dentine. Result: Observation under SEM showed that the crystals occluded the dentinal tubules and an enamel prism-like tissue formed on the demineralized dentine surface. XRD and FTIR analyses suggested that the crystals were hydroxyapatite. No crack was observed in the nanoscratch test. Conclusion: In this study, an in vitro agarose hydrogel system was successfully developed to regenerate enamel prism-like tissue on demineralised dentine surface. | - |
dc.language | eng | en_US |
dc.publisher | Sage Publications, Inc. The Journal's web site is located at http://www.sagepub.com/journalsProdDesc.nav?prodId=Journal201925 | en_US |
dc.relation.ispartof | Journal of Dental Research | en_US |
dc.rights | Journal of Dental Research. Copyright © Sage Publications, Inc. | en_US |
dc.subject | Collagen | - |
dc.subject | Dentin | - |
dc.subject | Enamel | - |
dc.subject | Regeneration and Remineralization | - |
dc.title | In-vitro agarose hydrogel system for biomimetic mineralisation on demineralised dentine | en_US |
dc.type | Conference_Paper | en_US |
dc.identifier.email | Lo, ECM: edward-lo@hku.hk | en_US |
dc.identifier.email | Chu, CH: chchu@hku.hk | en_US |
dc.identifier.authority | Lo, ECM=rp00015 | en_US |
dc.identifier.authority | Chu, CH=rp00022 | en_US |
dc.identifier.hkuros | 231074 | en_US |
dc.identifier.volume | 93 | en_US |
dc.identifier.issue | Special issue B: abstract no. 1207 | en_US |
dc.publisher.place | United States | en_US |
dc.identifier.issnl | 0022-0345 | - |