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Book Chapter: Fully Automatable Multidimensional Reversed-Phase Liquid Chromatography with Online Tandem Mass Spectrometry

TitleFully Automatable Multidimensional Reversed-Phase Liquid Chromatography with Online Tandem Mass Spectrometry
Authors
Lam, PYLaw, CHQuan, QZhao, YChu, IK
KeywordsMultidimensional liquid chromatography
Peptide separation
RP-RP
SCX-RP
Issue Date2014
PublisherHumana Press
Citation
Fully Automatable Multidimensional Reversed-Phase Liquid Chromatography with Online Tandem Mass Spectrometry. In Martins-de-Souza, D (Ed.), Shotgun Proteomics: Methods and Protocols, p. 39-51. New York: Humana Press, 2014 How to Cite?
DOI: http://dx.doi.org/10.1007/978-1-4939-0685-7_3
AbstractLiquid chromatography (LC) is essential for sample fractionation in shotgun proteomics applications. With suitable design, common LC separation chemistries, including reversed-phase (RP) and strong cation exchange (SCX) mode, can be combined in online multidimensional LC to greatly enhance the overall separation power and, thus, proteome coverage. This protocol describes the design and assembly of a flexible online multidimensional RP-SCX-RP LC system that is compatible with deep proteome profiling on common shotgun proteomics platforms.
Persistent Identifierhttp://hdl.handle.net/10722/205457
ISBN
9781493906840
ISSN
1064-3745
2020 SCImago Journal Rankings: 0.711
Series/Report no.Methods in Molecular Biology, vol. 1156

 

DC FieldValueLanguage
dc.contributor.authorLam, PYen_US
dc.contributor.authorLaw, CHen_US
dc.contributor.authorQuan, Qen_US
dc.contributor.authorZhao, Yen_US
dc.contributor.authorChu, IKen_US
dc.date.accessioned2014-09-20T02:34:40Z-
dc.date.available2014-09-20T02:34:40Z-
dc.date.issued2014en_US
dc.identifier.citationFully Automatable Multidimensional Reversed-Phase Liquid Chromatography with Online Tandem Mass Spectrometry. In Martins-de-Souza, D (Ed.), Shotgun Proteomics: Methods and Protocols, p. 39-51. New York: Humana Press, 2014en_US
dc.identifier.isbn9781493906840en_US
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/205457-
dc.description.abstractLiquid chromatography (LC) is essential for sample fractionation in shotgun proteomics applications. With suitable design, common LC separation chemistries, including reversed-phase (RP) and strong cation exchange (SCX) mode, can be combined in online multidimensional LC to greatly enhance the overall separation power and, thus, proteome coverage. This protocol describes the design and assembly of a flexible online multidimensional RP-SCX-RP LC system that is compatible with deep proteome profiling on common shotgun proteomics platforms.en_US
dc.languageengen_US
dc.publisherHumana Pressen_US
dc.relation.ispartofShotgun Proteomics: Methods and Protocolsen_US
dc.relation.ispartofseriesMethods in Molecular Biology, vol. 1156-
dc.subjectMultidimensional liquid chromatography-
dc.subjectPeptide separation-
dc.subjectRP-RP-
dc.subjectSCX-RP-
dc.titleFully Automatable Multidimensional Reversed-Phase Liquid Chromatography with Online Tandem Mass Spectrometryen_US
dc.typeBook_Chapteren_US
dc.identifier.emailChu, IK: ivankchu@hku.hken_US
dc.identifier.authorityChu, IK=rp00683en_US
dc.identifier.doi10.1007/978-1-4939-0685-7_3en_US
dc.identifier.scopuseid_2-s2.0-84918535720-
dc.identifier.hkuros239004en_US
dc.identifier.spage39en_US
dc.identifier.epage51en_US
dc.identifier.eissn1940-6029-
dc.publisher.placeNew Yorken_US
dc.identifier.issnl1064-3745-

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