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- Publisher Website: 10.1007/978-1-62703-755-6_7
- Scopus: eid_2-s2.0-84934439653
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Book Chapter: Portable Two-Way Riboswitches: Design and Engineering
| Title | Portable Two-Way Riboswitches: Design and Engineering |
|---|---|
| Authors | |
| Keywords | LacI LacP Portable two-way regulation Riboswitch |
| Issue Date | 2014 |
| Publisher | Humana Press |
| Citation | Portable Two-Way Riboswitches: Design and Engineering. In Ogawa, A (Ed.), Artificial Riboswitches: Methods and Protocols, p. 93-106. New York: Humana Press, 2014 How to Cite? |
| Abstract | Riboswitches are RNA-based regulatory devices that mediate ligand-dependent control of gene expression. However, there has been limited success in rationally designing riboswitches. Moreover, most previous riboswitches are confined to a particular gene and only perform one-way regulation. Here, we describe a library screening strategy for efficient creation of ON riboswitches of lacI of Escherichia coli. An ON riboswitch of lacI is then integrated with the lac promoter, generating a hybrid device to achieve portable sequential OFF-and-ON gene regulation. |
| Persistent Identifier | http://hdl.handle.net/10722/205461 |
| ISBN | |
| ISSN | 2020 SCImago Journal Rankings: 0.711 |
| Series/Report no. | Methods in Molecular Biology; vol. 1111 |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Jin, Y | en_US |
| dc.contributor.author | Huang, J | en_US |
| dc.date.accessioned | 2014-09-20T02:35:38Z | - |
| dc.date.available | 2014-09-20T02:35:38Z | - |
| dc.date.issued | 2014 | en_US |
| dc.identifier.citation | Portable Two-Way Riboswitches: Design and Engineering. In Ogawa, A (Ed.), Artificial Riboswitches: Methods and Protocols, p. 93-106. New York: Humana Press, 2014 | en_US |
| dc.identifier.isbn | 9781627037549 | en_US |
| dc.identifier.issn | 1064-3745 | - |
| dc.identifier.uri | http://hdl.handle.net/10722/205461 | - |
| dc.description.abstract | Riboswitches are RNA-based regulatory devices that mediate ligand-dependent control of gene expression. However, there has been limited success in rationally designing riboswitches. Moreover, most previous riboswitches are confined to a particular gene and only perform one-way regulation. Here, we describe a library screening strategy for efficient creation of ON riboswitches of lacI of Escherichia coli. An ON riboswitch of lacI is then integrated with the lac promoter, generating a hybrid device to achieve portable sequential OFF-and-ON gene regulation. | - |
| dc.language | eng | en_US |
| dc.publisher | Humana Press | en_US |
| dc.relation.ispartof | Artificial Riboswitches: Methods and Protocols | en_US |
| dc.relation.ispartofseries | Methods in Molecular Biology; vol. 1111 | - |
| dc.subject | LacI | - |
| dc.subject | LacP | - |
| dc.subject | Portable two-way regulation | - |
| dc.subject | Riboswitch | - |
| dc.title | Portable Two-Way Riboswitches: Design and Engineering | en_US |
| dc.type | Book_Chapter | en_US |
| dc.identifier.email | Jin, Y: jinye@hkusua.hku.hk | en_US |
| dc.identifier.email | Huang, J: jdhuang@hku.hk | en_US |
| dc.identifier.authority | Huang, J=rp00451 | en_US |
| dc.identifier.doi | 10.1007/978-1-62703-755-6_7 | - |
| dc.identifier.scopus | eid_2-s2.0-84934439653 | - |
| dc.identifier.hkuros | 237848 | en_US |
| dc.identifier.spage | 93 | en_US |
| dc.identifier.epage | 106 | en_US |
| dc.identifier.eissn | 1940-6029 | - |
| dc.publisher.place | New York | - |
| dc.identifier.issnl | 1064-3745 | - |