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- Publisher Website: 10.1002/jmv.23955
- Scopus: eid_2-s2.0-84911806911
- WOS: WOS:000344179400025
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Article: A novel molecular test for influenza B virus detection and lineage differentiation
Title | A novel molecular test for influenza B virus detection and lineage differentiation |
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Authors | |
Keywords | Influenza B virus Lineage differentiation RT-PCR Victoria Yamagata |
Issue Date | 2014 |
Citation | Journal of Medical Virology, 2014, v. 86 n. 12, p. 2171-6 How to Cite? |
Abstract | Contemporary influenza B viruses are classified into two groups known as Yamagata and Victoria lineages. The co-circulation of two viral lineages in recent years urges for a robust and simple diagnostic test for detecting influenza B viruses and for lineage differentiation. In this study, a SYBR green-based asymmetric PCR assay has been developed for influenza B virus detection. Apart from identifying influenza B virus, the assay contains sequence-specific probes for lineage differentiation. This allows identifying influenza B virus and detecting influenza B viral lineage in a single reaction. The test has been evaluated by a panel of respiratory specimens. Of 108 influenza B virus-positive specimens, 105 (97%) were positive in this assay. None of the negative control respiratory specimens were positive in the test (N = 60). Viral lineages of all samples that are positive in the assay (N = 105) can also be classified correctly. These results suggest that this assay has a potential for routine influenza B virus surveillance. |
Persistent Identifier | http://hdl.handle.net/10722/207837 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Wong, KSC | en_US |
dc.contributor.author | Tsang, GCH | en_US |
dc.contributor.author | Chan, KH | en_US |
dc.contributor.author | Li, OTW | en_US |
dc.contributor.author | Peiris, JSM | en_US |
dc.contributor.author | Poon, LLM | en_US |
dc.date.accessioned | 2015-01-19T11:23:03Z | - |
dc.date.available | 2015-01-19T11:23:03Z | - |
dc.date.issued | 2014 | en_US |
dc.identifier.citation | Journal of Medical Virology, 2014, v. 86 n. 12, p. 2171-6 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/207837 | - |
dc.description.abstract | Contemporary influenza B viruses are classified into two groups known as Yamagata and Victoria lineages. The co-circulation of two viral lineages in recent years urges for a robust and simple diagnostic test for detecting influenza B viruses and for lineage differentiation. In this study, a SYBR green-based asymmetric PCR assay has been developed for influenza B virus detection. Apart from identifying influenza B virus, the assay contains sequence-specific probes for lineage differentiation. This allows identifying influenza B virus and detecting influenza B viral lineage in a single reaction. The test has been evaluated by a panel of respiratory specimens. Of 108 influenza B virus-positive specimens, 105 (97%) were positive in this assay. None of the negative control respiratory specimens were positive in the test (N = 60). Viral lineages of all samples that are positive in the assay (N = 105) can also be classified correctly. These results suggest that this assay has a potential for routine influenza B virus surveillance. | en_US |
dc.language | eng | en_US |
dc.relation.ispartof | Journal of Medical Virology | en_US |
dc.subject | Influenza B virus | - |
dc.subject | Lineage differentiation | - |
dc.subject | RT-PCR | - |
dc.subject | Victoria | - |
dc.subject | Yamagata | - |
dc.title | A novel molecular test for influenza B virus detection and lineage differentiation | en_US |
dc.type | Article | en_US |
dc.identifier.email | Wong, KSC: kschloe@hku.hk | en_US |
dc.identifier.email | Chan, KH: chankh2@hkucc.hku.hk | en_US |
dc.identifier.email | Li, OTW: litwo@hku.hk | en_US |
dc.identifier.email | Peiris, JSM: malik@hkucc.hku.hk | en_US |
dc.identifier.email | Poon, LLM: llmpoon@hkucc.hku.hk | en_US |
dc.identifier.authority | Chan, KH=rp01921 | en_US |
dc.identifier.authority | Peiris, JSM=rp00410 | en_US |
dc.identifier.authority | Poon, LLM=rp00484 | en_US |
dc.identifier.doi | 10.1002/jmv.23955 | en_US |
dc.identifier.scopus | eid_2-s2.0-84911806911 | - |
dc.identifier.hkuros | 242084 | en_US |
dc.identifier.volume | 86 | en_US |
dc.identifier.issue | 12 | en_US |
dc.identifier.spage | 2171 | en_US |
dc.identifier.epage | 6 | en_US |
dc.identifier.isi | WOS:000344179400025 | - |