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- Publisher Website: 10.1111/j.1524-475X.2009.00454.x
- Scopus: eid_2-s2.0-62449127652
- PMID: 19320886
- WOS: WOS:000264188600006
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Article: Migration of bone marrow-derived mesenchymal stem cells induced by tumor necrosis factor-alpha and its possible role in wound healing
Title | Migration of bone marrow-derived mesenchymal stem cells induced by tumor necrosis factor-alpha and its possible role in wound healing |
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Authors | |
Issue Date | 2009 |
Publisher | Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/WRR |
Citation | Wound Repair and Regeneration, 2009, v. 17 n. 2, p. 185-191 How to Cite? |
Abstract | We aimed to investigate the effect of tumor necrosis factor-alpha (TNF-alpha) on the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in the migration ability of mesenchymal stem cells (MSCs) in the context of wound healing. We also explored the role of p38 mitogen-activated protein kinase and extracellular signal-regulated kinase (ERK) signaling pathways in the migration of MSCs. MSCs were isolated from the bone marrow and cultured. Immunocytochemistry, Western blotting, and reverse transcription-polymerase chain reaction were used to observe the effect of TNF-alpha on the expression of ICAM-1 and VCAM-1 in MSCs. The chemotaxis effect of TNF-alpha on MSCs was investigated by the trans-well system and the inhibition effect of TNF-alpha using its antibody. Western blotting analysis was used to observe the activation of JAK-STAT and mitogen-activated protein kinase signaling pathways, and ERK was inhibited with PD98059 and p38 with SB203580 to observe the effect of TNF-alpha on MSC migration and ICAM-1 expression. The expression of ICAM-1 could be up-regulated by 50 microg/L TNF-alpha (p<0.05), whereas that of VCAM-1 remained unchanged (p>0.05). Also, TNF-alpha showed a chemotaxis effect by enhancing the migration ability of MSCs (p<0.05). TNF-alpha at 50 microg/L increased the expression of phospho-ERK and phospho-p38, and SB203580, but not PD98059, could suppress the chemotaxis effect and up-regulation of ICAM-1 induced by TNF-alpha in MSCs (p<0.05). Thus, TNF-alpha could up-regulate the expression of ICAM-1 in MSCs and enhance the cells' migration ability, and the p38 signaling pathway might be involved in the TNF-alpha-induced migration ability for a role in wound repair and regeneration. |
Persistent Identifier | http://hdl.handle.net/10722/209207 |
ISSN | 2023 Impact Factor: 3.8 2023 SCImago Journal Rankings: 0.802 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Fu, XB | - |
dc.contributor.author | Han, B | - |
dc.contributor.author | Cai, S | - |
dc.contributor.author | Lei, YH | - |
dc.contributor.author | Sun, TZ | - |
dc.contributor.author | Sheng, ZY | - |
dc.date.accessioned | 2015-04-09T08:20:17Z | - |
dc.date.available | 2015-04-09T08:20:17Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Wound Repair and Regeneration, 2009, v. 17 n. 2, p. 185-191 | - |
dc.identifier.issn | 1067-1927 | - |
dc.identifier.uri | http://hdl.handle.net/10722/209207 | - |
dc.description.abstract | We aimed to investigate the effect of tumor necrosis factor-alpha (TNF-alpha) on the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) in the migration ability of mesenchymal stem cells (MSCs) in the context of wound healing. We also explored the role of p38 mitogen-activated protein kinase and extracellular signal-regulated kinase (ERK) signaling pathways in the migration of MSCs. MSCs were isolated from the bone marrow and cultured. Immunocytochemistry, Western blotting, and reverse transcription-polymerase chain reaction were used to observe the effect of TNF-alpha on the expression of ICAM-1 and VCAM-1 in MSCs. The chemotaxis effect of TNF-alpha on MSCs was investigated by the trans-well system and the inhibition effect of TNF-alpha using its antibody. Western blotting analysis was used to observe the activation of JAK-STAT and mitogen-activated protein kinase signaling pathways, and ERK was inhibited with PD98059 and p38 with SB203580 to observe the effect of TNF-alpha on MSC migration and ICAM-1 expression. The expression of ICAM-1 could be up-regulated by 50 microg/L TNF-alpha (p<0.05), whereas that of VCAM-1 remained unchanged (p>0.05). Also, TNF-alpha showed a chemotaxis effect by enhancing the migration ability of MSCs (p<0.05). TNF-alpha at 50 microg/L increased the expression of phospho-ERK and phospho-p38, and SB203580, but not PD98059, could suppress the chemotaxis effect and up-regulation of ICAM-1 induced by TNF-alpha in MSCs (p<0.05). Thus, TNF-alpha could up-regulate the expression of ICAM-1 in MSCs and enhance the cells' migration ability, and the p38 signaling pathway might be involved in the TNF-alpha-induced migration ability for a role in wound repair and regeneration. | - |
dc.language | eng | - |
dc.publisher | Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journals/WRR | - |
dc.relation.ispartof | Wound Repair and Regeneration | - |
dc.rights | The definitive version is available at www.blackwell-synergy.com | - |
dc.title | Migration of bone marrow-derived mesenchymal stem cells induced by tumor necrosis factor-alpha and its possible role in wound healing | - |
dc.type | Article | - |
dc.identifier.email | Cai, S: caisa@HKUCC-COM.hku.hk | - |
dc.identifier.doi | 10.1111/j.1524-475X.2009.00454.x | - |
dc.identifier.pmid | 19320886 | - |
dc.identifier.scopus | eid_2-s2.0-62449127652 | - |
dc.identifier.hkuros | 180798 | - |
dc.identifier.volume | 17 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 185 | - |
dc.identifier.epage | 191 | - |
dc.identifier.isi | WOS:000264188600006 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1067-1927 | - |