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Conference Paper: Expression of EPH-EPHRIN signalling in osteocytes under mechanical loading
Title | Expression of EPH-EPHRIN signalling in osteocytes under mechanical loading |
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Authors | |
Issue Date | 2015 |
Citation | The 91st Congress of The European Orthodontic Society (EOS 2015), Venice, Italy, 13-18 June, 2015. How to Cite? |
Abstract | AIMS: To investigate the effect of mechanical loading on ephrinB2-EphB4 and ephrinA2-EphA2 signalling pathways in the osteocyte-like MLO-Y4 cell line. MATERIALS AND METHOD: MLO-Y4 cells were seeded on collagen-coated plates and loaded with a force of 2000 μ strain for 1 hour, while the cells treated with no force loading served as the control. The cells were collected and analysed by real-time polymerase chain reaction to assess the expression of EphB4, ephrinB2, EphA2, and ephrinA2. All data were expressed as the mean standard deviations from three independent experiments. t-tests were used for two-sample comparisons. Significant differences were indicated as P < 0.05. RESULTS: Both ephrinB2-EphB4 and ephrinA2-EphA2 signalling pathways were altered by mechanical stress stimulation. The mRNA expression level of EphB4 in osteocytes was significantly increased after 1 hour of mechanical loading (P < 0.05), and ephrinA2 was also up-regulated under mechanical stress stimulation (P < 0.05). CONCLUSION: Mechanical stress alters Eph-ephrin signalling in osteocytes, with both ephrinB2-EphB4 and ephrinA2-EphA2 signalling pathways regulated, which indicates a dynamic balance between bone formation and bone resorption. |
Description | Scientific Posters: no. SP271 |
Persistent Identifier | http://hdl.handle.net/10722/214773 |
DC Field | Value | Language |
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dc.contributor.author | Liao, C | - |
dc.contributor.author | Zhang, C | - |
dc.contributor.author | Jin, L | - |
dc.contributor.author | Matsuo, K | - |
dc.contributor.author | Yang, Y | - |
dc.date.accessioned | 2015-08-21T11:55:07Z | - |
dc.date.available | 2015-08-21T11:55:07Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | The 91st Congress of The European Orthodontic Society (EOS 2015), Venice, Italy, 13-18 June, 2015. | - |
dc.identifier.uri | http://hdl.handle.net/10722/214773 | - |
dc.description | Scientific Posters: no. SP271 | - |
dc.description.abstract | AIMS: To investigate the effect of mechanical loading on ephrinB2-EphB4 and ephrinA2-EphA2 signalling pathways in the osteocyte-like MLO-Y4 cell line. MATERIALS AND METHOD: MLO-Y4 cells were seeded on collagen-coated plates and loaded with a force of 2000 μ strain for 1 hour, while the cells treated with no force loading served as the control. The cells were collected and analysed by real-time polymerase chain reaction to assess the expression of EphB4, ephrinB2, EphA2, and ephrinA2. All data were expressed as the mean standard deviations from three independent experiments. t-tests were used for two-sample comparisons. Significant differences were indicated as P < 0.05. RESULTS: Both ephrinB2-EphB4 and ephrinA2-EphA2 signalling pathways were altered by mechanical stress stimulation. The mRNA expression level of EphB4 in osteocytes was significantly increased after 1 hour of mechanical loading (P < 0.05), and ephrinA2 was also up-regulated under mechanical stress stimulation (P < 0.05). CONCLUSION: Mechanical stress alters Eph-ephrin signalling in osteocytes, with both ephrinB2-EphB4 and ephrinA2-EphA2 signalling pathways regulated, which indicates a dynamic balance between bone formation and bone resorption. | - |
dc.language | eng | - |
dc.relation.ispartof | Congress of the European Orthodontic Society, EOS 2015 | - |
dc.title | Expression of EPH-EPHRIN signalling in osteocytes under mechanical loading | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Zhang, C: zhangcf@hku.hk | - |
dc.identifier.email | Jin, L: ljjin@hkucc.hku.hk | - |
dc.identifier.email | Yang, Y: yangyanq@hku.hk | - |
dc.identifier.authority | Zhang, C=rp01408 | - |
dc.identifier.authority | Jin, L=rp00028 | - |
dc.identifier.authority | Yang, Y=rp00045 | - |
dc.identifier.hkuros | 247605 | - |