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- Publisher Website: 10.1073/pnas.0900191106
- Scopus: eid_2-s2.0-63849172494
- PMID: 19255428
- WOS: WOS:000264522600011
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Article: Identifying the proteins to which small-molecule probes and drugs bind in cells
Title | Identifying the proteins to which small-molecule probes and drugs bind in cells |
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Authors | |
Keywords | SILAC Small molecules Target identification |
Issue Date | 2009 |
Publisher | National Academy of Sciences. The Journal's web site is located at http://www.pnas.org |
Citation | Proceedings of the National Academy of Sciences, 2009, v. 106 n. 12, p. 4617-4622 How to Cite? |
Abstract | Most small-molecule probes and drugs alter cell circuitry by interacting with 1 or more proteins. A complete understanding of the interacting proteins and their associated protein complexes, whether the compounds are discovered by cell-based phenotypic or target- based screens, is extremely rare. Such a capability is expected to be highly illuminating-providing strong clues to the mechanisms used by small-molecules to achieve their recognized actions and suggesting potential unrecognized actions. We describe a powerful method combining quantitative proteomics(SILAC) with affinity enrichment to provide unbiased, robust and comprehensive identification of the proteins that bind to small-molecule probes and drugs. The method is scalable and general, requiring little optimization across different compound classes, and has already had a transformative effect on our studies of small-molecule probes. Here, we describe in full detail the application of the method to identify targets of kinase inhibitors and immunophilin binders. |
Persistent Identifier | http://hdl.handle.net/10722/221461 |
ISSN | 2021 Impact Factor: 12.779 2020 SCImago Journal Rankings: 5.011 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Ong, SE | - |
dc.contributor.author | Schenone, M | - |
dc.contributor.author | Margolin, AA | - |
dc.contributor.author | Li, X | - |
dc.contributor.author | Do, K | - |
dc.contributor.author | Doud, MK | - |
dc.contributor.author | Mani, DR | - |
dc.contributor.author | Kuai, L | - |
dc.contributor.author | Wang, X | - |
dc.contributor.author | Wood, JL | - |
dc.contributor.author | Tolliday, NJ | - |
dc.contributor.author | Koehler, AN | - |
dc.contributor.author | Marcaurelle, LA | - |
dc.contributor.author | Golub, TR | - |
dc.contributor.author | Gould, RJ | - |
dc.contributor.author | Schreiber, SL | - |
dc.contributor.author | Carr, SA | - |
dc.date.accessioned | 2015-11-26T08:07:36Z | - |
dc.date.available | 2015-11-26T08:07:36Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Proceedings of the National Academy of Sciences, 2009, v. 106 n. 12, p. 4617-4622 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | http://hdl.handle.net/10722/221461 | - |
dc.description.abstract | Most small-molecule probes and drugs alter cell circuitry by interacting with 1 or more proteins. A complete understanding of the interacting proteins and their associated protein complexes, whether the compounds are discovered by cell-based phenotypic or target- based screens, is extremely rare. Such a capability is expected to be highly illuminating-providing strong clues to the mechanisms used by small-molecules to achieve their recognized actions and suggesting potential unrecognized actions. We describe a powerful method combining quantitative proteomics(SILAC) with affinity enrichment to provide unbiased, robust and comprehensive identification of the proteins that bind to small-molecule probes and drugs. The method is scalable and general, requiring little optimization across different compound classes, and has already had a transformative effect on our studies of small-molecule probes. Here, we describe in full detail the application of the method to identify targets of kinase inhibitors and immunophilin binders. | - |
dc.language | eng | - |
dc.publisher | National Academy of Sciences. The Journal's web site is located at http://www.pnas.org | - |
dc.relation.ispartof | Proceedings of the National Academy of Sciences | - |
dc.rights | Proceedings of the National Academy of Sciences. Copyright © National Academy of Sciences. | - |
dc.subject | SILAC | - |
dc.subject | Small molecules | - |
dc.subject | Target identification | - |
dc.title | Identifying the proteins to which small-molecule probes and drugs bind in cells | - |
dc.type | Article | - |
dc.identifier.email | Li, X: xiaoyuli@hku.hk | - |
dc.identifier.authority | Li, X=rp02080 | - |
dc.identifier.doi | 10.1073/pnas.0900191106 | - |
dc.identifier.pmid | 19255428 | - |
dc.identifier.pmcid | PMC2649954 | - |
dc.identifier.scopus | eid_2-s2.0-63849172494 | - |
dc.identifier.volume | 106 | - |
dc.identifier.issue | 12 | - |
dc.identifier.spage | 4617 | - |
dc.identifier.epage | 4622 | - |
dc.identifier.isi | WOS:000264522600011 | - |
dc.publisher.place | United States | - |
dc.identifier.f1000 | 1158172 | - |
dc.identifier.issnl | 0027-8424 | - |