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Article: Tsukamurella hongkongensis sp. nov. and Tsukamurella sinensis sp. nov., isolated from patients with keratitis, catheter-related bacteraemia and conjunctivitis in Hong Kong

TitleTsukamurella hongkongensis sp. nov. and Tsukamurella sinensis sp. nov., isolated from patients with keratitis, catheter-related bacteraemia and conjunctivitis in Hong Kong
Authors
Issue Date2016
PublisherSociety for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.org
Citation
International Journal of Systematic and Evolutionary Microbiology, 2016, v. 66 n. 1, p. 391-397 How to Cite?
AbstractThree bacterial strains, HKU51T, HKU52T and HKU53, were isolated from a conjunctival swab, corneal scraping and blood culture of three patients in Hong Kong with conjunctivitis, keratitis and catheter-related bacteraemia, respectively. Cells were Gram-stain-positive, aerobic, catalase-positive, non-sporulating and non-motile bacilli. The three strains had unique biochemical profiles that were distinguishable from those of closely related species of the genus Tsukamurella. Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of Tsukamurella. 16S rRNA gene sequence analysis revealed 100 % sequence identity between HKU52T and HKU53, and the two strains shared 99.5 % sequence identity with Tsukamurella sunchonensis JCM 15929T and Tsukamurella pseudospumae JCM 13375T; HKU51T shared 99.6 % sequence identity with Tsukamurella pulmonis CCUG 35732T. The DNA G+C contents of strains HKU51T, HKU52T and HKU53 were 70.9±2.2, 71.3±2.1 and 71.2±2.3 mol% (mean±SD; n53), respectively. DNA–DNA hybridization confirmed that the novel strains were distinct from other known species of the genus Tsukamurella (≤50.1±3.7 % DNA–DNA relatedness); two of the isolates, HKU52T and HKU53, represented the same species (≥94.6±5.6 % DNA–DNA relatedness), while the third isolate, HKU51T, represented another species. The novel species Tsukamurella hongkongensis sp. nov. is proposed to accommodate strains HKU52T and HKU53, with HKU52T (=JCM 30715T=DSM 100208T) as the type strain; whilst another novel species, Tsukamurella sinensis sp. nov., is proposed to accommodate the third isolate, HKU51T (=JCM 30714T=DSM 100207T), which is designated the type strain.
Persistent Identifierhttp://hdl.handle.net/10722/223225
ISSN
2017 Impact Factor: 1.932
2015 SCImago Journal Rankings: 1.088
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTeng, JLL-
dc.contributor.authorTang, Y-
dc.contributor.authorWong, SSY-
dc.contributor.authorNgan, AHY-
dc.contributor.authorHuang, Y-
dc.contributor.authorTsang, CC-
dc.contributor.authorChoi, GKY-
dc.contributor.authorLau, SKP-
dc.contributor.authorWoo, PCY-
dc.date.accessioned2016-02-23T01:55:45Z-
dc.date.available2016-02-23T01:55:45Z-
dc.date.issued2016-
dc.identifier.citationInternational Journal of Systematic and Evolutionary Microbiology, 2016, v. 66 n. 1, p. 391-397-
dc.identifier.issn1466-5026-
dc.identifier.urihttp://hdl.handle.net/10722/223225-
dc.description.abstractThree bacterial strains, HKU51T, HKU52T and HKU53, were isolated from a conjunctival swab, corneal scraping and blood culture of three patients in Hong Kong with conjunctivitis, keratitis and catheter-related bacteraemia, respectively. Cells were Gram-stain-positive, aerobic, catalase-positive, non-sporulating and non-motile bacilli. The three strains had unique biochemical profiles that were distinguishable from those of closely related species of the genus Tsukamurella. Fatty acids, mycolic acids, cell-wall sugars and peptidoglycan analyses showed that they were typical of members of Tsukamurella. 16S rRNA gene sequence analysis revealed 100 % sequence identity between HKU52T and HKU53, and the two strains shared 99.5 % sequence identity with Tsukamurella sunchonensis JCM 15929T and Tsukamurella pseudospumae JCM 13375T; HKU51T shared 99.6 % sequence identity with Tsukamurella pulmonis CCUG 35732T. The DNA G+C contents of strains HKU51T, HKU52T and HKU53 were 70.9±2.2, 71.3±2.1 and 71.2±2.3 mol% (mean±SD; n53), respectively. DNA–DNA hybridization confirmed that the novel strains were distinct from other known species of the genus Tsukamurella (≤50.1±3.7 % DNA–DNA relatedness); two of the isolates, HKU52T and HKU53, represented the same species (≥94.6±5.6 % DNA–DNA relatedness), while the third isolate, HKU51T, represented another species. The novel species Tsukamurella hongkongensis sp. nov. is proposed to accommodate strains HKU52T and HKU53, with HKU52T (=JCM 30715T=DSM 100208T) as the type strain; whilst another novel species, Tsukamurella sinensis sp. nov., is proposed to accommodate the third isolate, HKU51T (=JCM 30714T=DSM 100207T), which is designated the type strain.-
dc.languageeng-
dc.publisherSociety for General Microbiology. The Journal's web site is located at http://ijs.sgmjournals.org-
dc.relation.ispartofInternational Journal of Systematic and Evolutionary Microbiology-
dc.rightsInternational Journal of Systematic and Evolutionary Microbiology. Copyright © Society for General Microbiology.-
dc.rightsThis is an author manuscript that has been accepted for publication in [Journal Title], copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in [Journal Title]. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of 'Fair Use of Copyrighted Materials' (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at [Journal URL], and is freely available without a subscription.-
dc.titleTsukamurella hongkongensis sp. nov. and Tsukamurella sinensis sp. nov., isolated from patients with keratitis, catheter-related bacteraemia and conjunctivitis in Hong Kong-
dc.typeArticle-
dc.identifier.emailTeng, JLL: llteng@hku.hk-
dc.identifier.emailWong, SSY: samsonsy@hkucc.hku.hk-
dc.identifier.emailTsang, CC: microbioct@connect.hku.hk-
dc.identifier.emailLau, SKP: skplau@hkucc.hku.hk-
dc.identifier.emailWoo, PCY: pcywoo@hkucc.hku.hk-
dc.identifier.authorityTeng, JLL=rp00277-
dc.identifier.authorityWong, SSY=rp00395-
dc.identifier.authorityTsang, CC=rp02492-
dc.identifier.authorityLau, SKP=rp00486-
dc.identifier.authorityWoo, PCY=rp00430-
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1099/ijsem.0.000733-
dc.identifier.pmid26530900-
dc.identifier.scopuseid_2-s2.0-84957831329-
dc.identifier.hkuros256967-
dc.identifier.volume66-
dc.identifier.issue1-
dc.identifier.spage391-
dc.identifier.epage397-
dc.identifier.isiWOS:000371452900056-
dc.publisher.placeUnited Kingdom-

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