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- Publisher Website: 10.1038/srep22824
- Scopus: eid_2-s2.0-84960341699
- PMID: 26956215
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Article: Mechanical oscillations enhance gene delivery into suspended cells
Title | Mechanical oscillations enhance gene delivery into suspended cells |
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Authors | |
Issue Date | 2016 |
Publisher | Nature Publishing Group: Open Access Journals. The Journal's web site is located at http://www.nature.com/srep/index.html |
Citation | Scientific Reports, 2016, v. 6, article no. 22824, p. 1-9 How to Cite? |
Abstract | Suspended cells are difficult to be transfected by common biochemical methods which require cell attachment to a substrate. Mechanical oscillations of suspended cells at certain frequencies are found to result in significant increase in membrane permeability and potency for delivery of nano-particles and genetic materials into the cells. Nanomaterials including siRNAs are found to penetrate into suspended cells after subjecting to short-time mechanical oscillations, which would otherwise not affect the viability of the cells. Theoretical analysis indicates significant deformation of the actin-filament network in the cytoskeleton cortex during mechanical oscillations at the experimental frequency, which is likely to rupture the soft phospholipid bilayer leading to increased membrane permeability. The results here indicate a new method for enhancing cell transfection. |
Persistent Identifier | http://hdl.handle.net/10722/227340 |
ISSN | 2021 Impact Factor: 4.996 2020 SCImago Journal Rankings: 1.240 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhou, Z | - |
dc.contributor.author | Sun, X | - |
dc.contributor.author | Ma, J | - |
dc.contributor.author | Man, CH | - |
dc.contributor.author | Wong, AST | - |
dc.contributor.author | Leung, AYH | - |
dc.contributor.author | Ngan, AHW | - |
dc.date.accessioned | 2016-07-18T09:09:53Z | - |
dc.date.available | 2016-07-18T09:09:53Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Scientific Reports, 2016, v. 6, article no. 22824, p. 1-9 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.uri | http://hdl.handle.net/10722/227340 | - |
dc.description.abstract | Suspended cells are difficult to be transfected by common biochemical methods which require cell attachment to a substrate. Mechanical oscillations of suspended cells at certain frequencies are found to result in significant increase in membrane permeability and potency for delivery of nano-particles and genetic materials into the cells. Nanomaterials including siRNAs are found to penetrate into suspended cells after subjecting to short-time mechanical oscillations, which would otherwise not affect the viability of the cells. Theoretical analysis indicates significant deformation of the actin-filament network in the cytoskeleton cortex during mechanical oscillations at the experimental frequency, which is likely to rupture the soft phospholipid bilayer leading to increased membrane permeability. The results here indicate a new method for enhancing cell transfection. | - |
dc.language | eng | - |
dc.publisher | Nature Publishing Group: Open Access Journals. The Journal's web site is located at http://www.nature.com/srep/index.html | - |
dc.relation.ispartof | Scientific Reports | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.title | Mechanical oscillations enhance gene delivery into suspended cells | - |
dc.type | Article | - |
dc.identifier.email | Zhou, Z: zlzhou@hku.hk | - |
dc.identifier.email | Man, CH: csman729@hku.hk | - |
dc.identifier.email | Wong, AST: awong1@hku.hk | - |
dc.identifier.email | Leung, AYH: ayhleung@hku.hk | - |
dc.identifier.email | Ngan, AHW: hwngan@hku.hk | - |
dc.identifier.authority | Man, CH=rp02543 | - |
dc.identifier.authority | Wong, AST=rp00805 | - |
dc.identifier.authority | Leung, AYH=rp00265 | - |
dc.identifier.authority | Ngan, AHW=rp00225 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1038/srep22824 | - |
dc.identifier.pmid | 26956215 | - |
dc.identifier.pmcid | PMC4783776 | - |
dc.identifier.scopus | eid_2-s2.0-84960341699 | - |
dc.identifier.hkuros | 259359 | - |
dc.identifier.volume | 6 | - |
dc.identifier.spage | article no. 22824, p. 1 | - |
dc.identifier.epage | article no. 22824, p. 9 | - |
dc.identifier.isi | WOS:000371627500001 | - |
dc.publisher.place | United Kingdom | - |
dc.identifier.issnl | 2045-2322 | - |