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Article: Mutagenesis and genome engineering of Epstein-Barr virus in cultured human cells by CRISPR/Cas9
Title | Mutagenesis and genome engineering of Epstein-Barr virus in cultured human cells by CRISPR/Cas9 |
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Authors | |
Keywords | Cure of Epstein–Barr virus infection Episomal viral DNA genome Epstein–Barr virus Genetic analysis of Epstein–Barr virus RNA-guided genome editing |
Issue Date | 2016 |
Publisher | Humana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651 |
Citation | Methods in Molecular Biology, 2016, v. 1498, p. 23-31 How to Cite? |
Abstract | The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 nuclease (Cas9) system is a powerful genome-editing tool for both chromosomal and extrachromosomal DNA. DNA viruses such as Epstein–Barr virus (EBV), which undergoes episomal replication in human cells, can be effectively edited by CRISPR/Cas9. We have demonstrated targeted editing of the EBV genome by CRISPR/Cas9 in several lines of EBV-infected cells. CRISPR/Cas9-based mutagenesis and genome engineering of EBV provides a new method for genetic analysis, which has some advantages over bacterial artificial chromosome-based recombineering. This approach might also prove useful in the cure of EBV infection. In this chapter, we use the knockout of the BART promoter as an example to detail the experimental procedures for construction of recombinant EBV in human cells. |
Persistent Identifier | http://hdl.handle.net/10722/238618 |
ISBN | |
ISSN | 2023 SCImago Journal Rankings: 0.399 |
DC Field | Value | Language |
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dc.contributor.author | Yuen, KS | - |
dc.contributor.author | Chan, CP | - |
dc.contributor.author | Kok, KH | - |
dc.contributor.author | Jin, D | - |
dc.date.accessioned | 2017-02-20T01:23:52Z | - |
dc.date.available | 2017-02-20T01:23:52Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Methods in Molecular Biology, 2016, v. 1498, p. 23-31 | - |
dc.identifier.isbn | 9781493964727 | - |
dc.identifier.issn | 1064-3745 | - |
dc.identifier.uri | http://hdl.handle.net/10722/238618 | - |
dc.description.abstract | The clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 nuclease (Cas9) system is a powerful genome-editing tool for both chromosomal and extrachromosomal DNA. DNA viruses such as Epstein–Barr virus (EBV), which undergoes episomal replication in human cells, can be effectively edited by CRISPR/Cas9. We have demonstrated targeted editing of the EBV genome by CRISPR/Cas9 in several lines of EBV-infected cells. CRISPR/Cas9-based mutagenesis and genome engineering of EBV provides a new method for genetic analysis, which has some advantages over bacterial artificial chromosome-based recombineering. This approach might also prove useful in the cure of EBV infection. In this chapter, we use the knockout of the BART promoter as an example to detail the experimental procedures for construction of recombinant EBV in human cells. | - |
dc.language | eng | - |
dc.publisher | Humana Press, Inc. The Journal's web site is located at http://link.springer.com/bookseries/7651 | - |
dc.relation.ispartof | Methods in Molecular Biology | - |
dc.rights | The final publication is available at Springer via http://dx.doi.org/10.1007/978-1-4939-6472-7_2 | - |
dc.subject | Cure of Epstein–Barr virus infection | - |
dc.subject | Episomal viral DNA genome | - |
dc.subject | Epstein–Barr virus | - |
dc.subject | Genetic analysis of Epstein–Barr virus | - |
dc.subject | RNA-guided genome editing | - |
dc.title | Mutagenesis and genome engineering of Epstein-Barr virus in cultured human cells by CRISPR/Cas9 | - |
dc.type | Article | - |
dc.identifier.email | Yuen, KS: samyuen@hku.hk | - |
dc.identifier.email | Chan, CP: chancp10@hku.hk | - |
dc.identifier.email | Kok, KH: khkok@hku.hk | - |
dc.identifier.email | Jin, D: dyjin@hku.hk | - |
dc.identifier.authority | Chan, CP=rp02031 | - |
dc.identifier.authority | Kok, KH=rp01455 | - |
dc.identifier.authority | Jin, D=rp00452 | - |
dc.description.nature | postprint | - |
dc.identifier.doi | 10.1007/978-1-4939-6472-7_2 | - |
dc.identifier.scopus | eid_2-s2.0-84990957221 | - |
dc.identifier.hkuros | 271259 | - |
dc.identifier.volume | 1498 | - |
dc.identifier.spage | 23 | - |
dc.identifier.epage | 31 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1064-3745 | - |