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- Publisher Website: 10.3168/jds.2015-10718
- Scopus: eid_2-s2.0-84957917006
- PMID: 26874411
- WOS: WOS:000372945500017
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Article: Effect of salt stress on morphology and membrane composition of Lactobacillus acidophilus, Lactobacillus casei, and Bifidobacterium bifidum, and their adhesion to human intestinal epithelial-like Caco-2 cells
Title | Effect of salt stress on morphology and membrane composition of Lactobacillus acidophilus, Lactobacillus casei, and Bifidobacterium bifidum, and their adhesion to human intestinal epithelial-like Caco-2 cells |
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Authors | |
Keywords | Potassium chloride substitution Phospholipids Transmission electron microscopy CaCO-2 |
Issue Date | 2016 |
Publisher | American Dairy Science Association. The Journal's web site is located at http://www.journalofdairyscience.org/ |
Citation | Journal of Dairy Science, 2016, v. 99 n. 4, p. 2594-2605 How to Cite? |
Abstract | The effects of NaCl reduction (10.0, 7.5, 5.0, 2.5, and 0% NaCl) and its substitution with KCl (50% substitution at each given concentration) on morphology of Lactobacillus acidophilus, Lactobacillus casei, and Bifidobacterium longum was investigated using transmission electron microscopy. Changes in membrane composition, including fatty acids and phospholipids, were investigated using gas chromatography and thin layer chromatography. Adhesion ability of these bacteria to human intestinal epithelial-like Caco-2 cells, as affected by NaCl and its substitution with KCl, was also evaluated. Bacteria appeared elongated and the intracellular content appeared contracted when subjected to salt stress, as observed by transmission electron microscopy. Fatty acid content was altered with an increase in the ratio of unsaturated to saturated fatty acid content on increasing the NaCl-induced stress. Among the phospholipids, phosphatidylglycerol was reduced, whereas phosphatidylinositol and cardioplipin were increased when the bacteria were subjected to salt stress. There was a significant reduction in adhesion ability of the bacteria to Caco-2 cells when cultured in media supplemented with NaCl; however, the adhesion ability was improved on substitution with KCl at a given total salt concentration. The findings provide insights into bacterial membrane damage caused by NaCl. |
Persistent Identifier | http://hdl.handle.net/10722/238772 |
ISSN | 2023 Impact Factor: 3.7 2023 SCImago Journal Rankings: 1.219 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Gandhi, A | - |
dc.contributor.author | Shah, N | - |
dc.date.accessioned | 2017-02-20T01:25:53Z | - |
dc.date.available | 2017-02-20T01:25:53Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Journal of Dairy Science, 2016, v. 99 n. 4, p. 2594-2605 | - |
dc.identifier.issn | 0022-0302 | - |
dc.identifier.uri | http://hdl.handle.net/10722/238772 | - |
dc.description.abstract | The effects of NaCl reduction (10.0, 7.5, 5.0, 2.5, and 0% NaCl) and its substitution with KCl (50% substitution at each given concentration) on morphology of Lactobacillus acidophilus, Lactobacillus casei, and Bifidobacterium longum was investigated using transmission electron microscopy. Changes in membrane composition, including fatty acids and phospholipids, were investigated using gas chromatography and thin layer chromatography. Adhesion ability of these bacteria to human intestinal epithelial-like Caco-2 cells, as affected by NaCl and its substitution with KCl, was also evaluated. Bacteria appeared elongated and the intracellular content appeared contracted when subjected to salt stress, as observed by transmission electron microscopy. Fatty acid content was altered with an increase in the ratio of unsaturated to saturated fatty acid content on increasing the NaCl-induced stress. Among the phospholipids, phosphatidylglycerol was reduced, whereas phosphatidylinositol and cardioplipin were increased when the bacteria were subjected to salt stress. There was a significant reduction in adhesion ability of the bacteria to Caco-2 cells when cultured in media supplemented with NaCl; however, the adhesion ability was improved on substitution with KCl at a given total salt concentration. The findings provide insights into bacterial membrane damage caused by NaCl. | - |
dc.language | eng | - |
dc.publisher | American Dairy Science Association. The Journal's web site is located at http://www.journalofdairyscience.org/ | - |
dc.relation.ispartof | Journal of Dairy Science | - |
dc.rights | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject | Potassium chloride substitution | - |
dc.subject | Phospholipids | - |
dc.subject | Transmission electron microscopy | - |
dc.subject | CaCO-2 | - |
dc.title | Effect of salt stress on morphology and membrane composition of Lactobacillus acidophilus, Lactobacillus casei, and Bifidobacterium bifidum, and their adhesion to human intestinal epithelial-like Caco-2 cells | - |
dc.type | Article | - |
dc.identifier.email | Shah, N: npshah@hku.hk | - |
dc.identifier.authority | Shah, N=rp01571 | - |
dc.description.nature | postprint | - |
dc.identifier.doi | 10.3168/jds.2015-10718 | - |
dc.identifier.pmid | 26874411 | - |
dc.identifier.scopus | eid_2-s2.0-84957917006 | - |
dc.identifier.hkuros | 271373 | - |
dc.identifier.volume | 99 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | 2594 | - |
dc.identifier.epage | 2605 | - |
dc.identifier.isi | WOS:000372945500017 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0022-0302 | - |