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Conference Paper: Decoding and Engineering Complex Biological Systems
Title | Decoding and Engineering Complex Biological Systems |
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Authors | |
Issue Date | 2016 |
Citation | Inaugural Symposium of School of Biomedical Sciences, The University of Hong Kong, Hong Kong, 19 May 2016 How to Cite? |
Abstract | The orchestrated action of genes controls complex biological traits, yet the systematic discovery of gene combinations that modulate these phenotypes in human cells is labor-intensive and challenging to scale. Harnessing the power of synthetic biology and next-generation sequencing technologies, we developed CombiGEM (Combinatorial Genetics En Masse) as a powerful platform for high-throughput functional characterization of combinatorial genetic perturbations in human cells. CombiGEM enables rapid, scalable assembly of high-order barcoded combinatorial genetic libraries, and multiplexed quantification of all library members by using next-generation sequencing technologies. The genetic elements included in CombiGEM libraries can be arbitrary, including microRNAs, gene expression/knockdown constructs, synthetic-biology circuit components, and programmable CRISPR-Cas genome editing tools. We envision that this technology platform will be applicable to a broad range of biological settings, and will enable the systematic identification of genetic combinations that regulate complex biological processes and diseases. |
Description | Oral Presentation 5 |
Persistent Identifier | http://hdl.handle.net/10722/239043 |
DC Field | Value | Language |
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dc.contributor.author | Wong, ASL | - |
dc.date.accessioned | 2017-03-01T04:08:29Z | - |
dc.date.available | 2017-03-01T04:08:29Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | Inaugural Symposium of School of Biomedical Sciences, The University of Hong Kong, Hong Kong, 19 May 2016 | - |
dc.identifier.uri | http://hdl.handle.net/10722/239043 | - |
dc.description | Oral Presentation 5 | - |
dc.description.abstract | The orchestrated action of genes controls complex biological traits, yet the systematic discovery of gene combinations that modulate these phenotypes in human cells is labor-intensive and challenging to scale. Harnessing the power of synthetic biology and next-generation sequencing technologies, we developed CombiGEM (Combinatorial Genetics En Masse) as a powerful platform for high-throughput functional characterization of combinatorial genetic perturbations in human cells. CombiGEM enables rapid, scalable assembly of high-order barcoded combinatorial genetic libraries, and multiplexed quantification of all library members by using next-generation sequencing technologies. The genetic elements included in CombiGEM libraries can be arbitrary, including microRNAs, gene expression/knockdown constructs, synthetic-biology circuit components, and programmable CRISPR-Cas genome editing tools. We envision that this technology platform will be applicable to a broad range of biological settings, and will enable the systematic identification of genetic combinations that regulate complex biological processes and diseases. | - |
dc.language | eng | - |
dc.relation.ispartof | University of Hong Kong, School of Biomedical Sciences Symposium | - |
dc.title | Decoding and Engineering Complex Biological Systems | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Wong, ASL: aslw@hku.hk | - |
dc.identifier.authority | Wong, ASL=rp02139 | - |
dc.identifier.hkuros | 264307 | - |