File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Cell-structure specific necrosis by optical-trap induced intracellular nuclear oscillation

TitleCell-structure specific necrosis by optical-trap induced intracellular nuclear oscillation
Authors
KeywordsFrequency
Leukemia
Mechanical force
Necrotic cell death
Optical trap
Issue Date2017
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/issn/17516161
Citation
Journal of the Mechanical Behavior of Biomedical Materials, 2017, v. 66, p. 58-67 How to Cite?
AbstractA drug-free procedure for killing malignant cells in a cell-type specific manner would represent a significant breakthrough for leukemia treatment. Here, we show that mechanically vibrating a cell in a specific oscillation condition can significantly promote necrosis. Specifically, oscillating the cell by a low-power laser trap at specific frequencies of a few Hz was found to result in increased death rate of 50% or above in different types of myelogenous leukemia cells, while normal leukocytes showed very little response to similar laser manipulations. The alteration of cell membrane permeability and cell volume, detected from ethidium bromide staining and measurement of intracellular sodium ion concentration, together with the observed membrane blebbing within 10 min, suggest cell necrosis. Mechanics modelling reveals severe distortion of the cytoskeleton cortex at frequencies in the same range for peaked cell death. The disruption of cell membrane leading to cell death is therefore due to the cortex distortion, and the frequency at which this becomes significant is cell-type specific. Our findings lay down a new concept for treating leukemia based on vibration induced disruption of membrane in targeted malignant cells.
Persistent Identifierhttp://hdl.handle.net/10722/243151
ISSN
2017 Impact Factor: 3.239
2015 SCImago Journal Rankings: 1.068
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSun, XX-
dc.contributor.authorZhou, ZL-
dc.contributor.authorMan, CH-
dc.contributor.authorLeung, AYH-
dc.contributor.authorNgan, AHW-
dc.date.accessioned2017-08-25T02:50:44Z-
dc.date.available2017-08-25T02:50:44Z-
dc.date.issued2017-
dc.identifier.citationJournal of the Mechanical Behavior of Biomedical Materials, 2017, v. 66, p. 58-67-
dc.identifier.issn1751-6161-
dc.identifier.urihttp://hdl.handle.net/10722/243151-
dc.description.abstractA drug-free procedure for killing malignant cells in a cell-type specific manner would represent a significant breakthrough for leukemia treatment. Here, we show that mechanically vibrating a cell in a specific oscillation condition can significantly promote necrosis. Specifically, oscillating the cell by a low-power laser trap at specific frequencies of a few Hz was found to result in increased death rate of 50% or above in different types of myelogenous leukemia cells, while normal leukocytes showed very little response to similar laser manipulations. The alteration of cell membrane permeability and cell volume, detected from ethidium bromide staining and measurement of intracellular sodium ion concentration, together with the observed membrane blebbing within 10 min, suggest cell necrosis. Mechanics modelling reveals severe distortion of the cytoskeleton cortex at frequencies in the same range for peaked cell death. The disruption of cell membrane leading to cell death is therefore due to the cortex distortion, and the frequency at which this becomes significant is cell-type specific. Our findings lay down a new concept for treating leukemia based on vibration induced disruption of membrane in targeted malignant cells.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/issn/17516161-
dc.relation.ispartofJournal of the Mechanical Behavior of Biomedical Materials-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectFrequency-
dc.subjectLeukemia-
dc.subjectMechanical force-
dc.subjectNecrotic cell death-
dc.subjectOptical trap-
dc.titleCell-structure specific necrosis by optical-trap induced intracellular nuclear oscillation-
dc.typeArticle-
dc.identifier.emailZhou, ZL: zlzhou@hku.hk-
dc.identifier.emailMan, CH: csman729@hku.hk-
dc.identifier.emailLeung, AYH: ayhleung@hku.hk-
dc.identifier.emailNgan, AHW: hwngan@hku.hk-
dc.identifier.authorityMan, CH=rp02543-
dc.identifier.authorityLeung, AYH=rp00265-
dc.identifier.authorityNgan, AHW=rp00225-
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.jmbbm.2016.10.020-
dc.identifier.pmid27838591-
dc.identifier.scopuseid_2-s2.0-84994875455-
dc.identifier.hkuros275310-
dc.identifier.volume66-
dc.identifier.spage58-
dc.identifier.epage67-
dc.identifier.isiWOS:000392570600008-
dc.publisher.placeNetherlands-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats