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Article: Marine Collagen Peptides Reduce Endothelial Cell Injury In Diabetic Rats By Inhibiting Apoptosis And The Expression Of Coupling Factor 6 And Microparticles

TitleMarine Collagen Peptides Reduce Endothelial Cell Injury In Diabetic Rats By Inhibiting Apoptosis And The Expression Of Coupling Factor 6 And Microparticles
Authors
Issue Date2017
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/mmr/
Citation
Molecular Medicine Reports, 2017, v. 16, p. 3947-3957 How to Cite?
AbstractThe present study aimed to elucidate the role of marine collagen peptides (MCPs) in protection of carotid artery vascular endothelial cells (CAVECs) in type 2 diabetes mellitus (T2DM), and the mechanism underlying this process. In an in vivo experiment, diabetic Wistar rats were divided randomly into four groups (n=10/group): Diabetes control, and three diabetes groups administered low, medium and high doses of MCPs (2.25, 4.5 and 9.0 g/kg body weight/day, respectively). Another 10 healthy rats served as the control. In an in vitro experiment, human umbilical‑vein endothelial cells (HUVECs) were incubated in normal and high concentrations of glucose with or without MCPs (3.0, 15.0 and 30.0 mg/ml, respectively) for 24, 48 or 72 h. Blood vessel/endothelial construction, inflammatory exudation and associated molecular biomarkers in CAVECs were detected and analyzed. The results of the present study demonstrated that in rats, MCP treatment for 4 weeks significantly lowered blood glucose and attenuated endothelial thinning and inflammatory exudation in carotid‑artery vascular endothelial cells. In vitro, the high‑glucose intervention significantly increased cell apoptosis in HUVECs, and medium and high doses of MCPs (4.5 and 9.0 g/kg body weight/day, respectively) partially ameliorated this high glucose‑mediated apoptosis and decreased levels of apoptosis biomarkers. In conclusion, a moderate oral MCP dose (≥4.5 g/kg body weight/day) may be a novel therapeutic tool to protect against early cardiovascular complications associated with T2DM by inhibiting apoptosis and reducing the expression of coupling factor 6 and microparticles. Key words: marine collagen peptide, coupling factor 6, microparticles, cell apoptosis, type 2 diabetes mellitus, cardiovascular complications
Persistent Identifierhttp://hdl.handle.net/10722/244403
ISSN
2017 Impact Factor: 1.922
2015 SCImago Journal Rankings: 0.637
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhu, CF-
dc.contributor.authorZhang, W-
dc.contributor.authorLiu, JG-
dc.contributor.authorMu, B-
dc.contributor.authorzhang, F-
dc.contributor.authorLai, NN-
dc.contributor.authorZhou, JX-
dc.contributor.authorXu, A-
dc.contributor.authorLi, Y-
dc.date.accessioned2017-09-18T01:51:49Z-
dc.date.available2017-09-18T01:51:49Z-
dc.date.issued2017-
dc.identifier.citationMolecular Medicine Reports, 2017, v. 16, p. 3947-3957-
dc.identifier.issn1791-2997-
dc.identifier.urihttp://hdl.handle.net/10722/244403-
dc.description.abstractThe present study aimed to elucidate the role of marine collagen peptides (MCPs) in protection of carotid artery vascular endothelial cells (CAVECs) in type 2 diabetes mellitus (T2DM), and the mechanism underlying this process. In an in vivo experiment, diabetic Wistar rats were divided randomly into four groups (n=10/group): Diabetes control, and three diabetes groups administered low, medium and high doses of MCPs (2.25, 4.5 and 9.0 g/kg body weight/day, respectively). Another 10 healthy rats served as the control. In an in vitro experiment, human umbilical‑vein endothelial cells (HUVECs) were incubated in normal and high concentrations of glucose with or without MCPs (3.0, 15.0 and 30.0 mg/ml, respectively) for 24, 48 or 72 h. Blood vessel/endothelial construction, inflammatory exudation and associated molecular biomarkers in CAVECs were detected and analyzed. The results of the present study demonstrated that in rats, MCP treatment for 4 weeks significantly lowered blood glucose and attenuated endothelial thinning and inflammatory exudation in carotid‑artery vascular endothelial cells. In vitro, the high‑glucose intervention significantly increased cell apoptosis in HUVECs, and medium and high doses of MCPs (4.5 and 9.0 g/kg body weight/day, respectively) partially ameliorated this high glucose‑mediated apoptosis and decreased levels of apoptosis biomarkers. In conclusion, a moderate oral MCP dose (≥4.5 g/kg body weight/day) may be a novel therapeutic tool to protect against early cardiovascular complications associated with T2DM by inhibiting apoptosis and reducing the expression of coupling factor 6 and microparticles. Key words: marine collagen peptide, coupling factor 6, microparticles, cell apoptosis, type 2 diabetes mellitus, cardiovascular complications-
dc.languageeng-
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/mmr/-
dc.relation.ispartofMolecular Medicine Reports-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleMarine Collagen Peptides Reduce Endothelial Cell Injury In Diabetic Rats By Inhibiting Apoptosis And The Expression Of Coupling Factor 6 And Microparticles-
dc.typeArticle-
dc.identifier.emailZhu, CF: deliazhu@hku.hk-
dc.identifier.emailXu, A: amxu@hkucc.hku.hk-
dc.identifier.authorityXu, A=rp00485-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3892/mmr.2017.7061-
dc.identifier.hkuros277783-
dc.identifier.hkuros277781-
dc.identifier.volume16-
dc.identifier.spage3947-
dc.identifier.epage3957-
dc.identifier.isiWOS:000410753800031-
dc.publisher.placeGreece-

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