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Conference Paper: Co-electrospray of mesemchymal stem cells and gene with non-viral vector in hydrogel microspheres for enhanced stem cell-based therapy
Title | Co-electrospray of mesemchymal stem cells and gene with non-viral vector in hydrogel microspheres for enhanced stem cell-based therapy |
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Authors | |
Issue Date | 2017 |
Publisher | The American Ceramic Society. |
Citation | 12th Pacific Rim Conference on Ceramic and Glass Technology (PACRIM 12), including Glass & Optical Materials Division Meeting (GOMD 2017), Waikoloa, Hawaii, USA, 21-26 May 2017. In Conference Program, p. 150 How to Cite? |
Abstract | Stem cell-based therapies are promising for treating human tissue loss. Mesenchymal stem cells (MSCs) are increasingly used. After delivery to target, MSCs need to have a suitable environment for
differentiation and their fate should be appropriately directed. One approach is to modify MSCs first via gene delivery and then encapsulate genetically engineered MSCs in a carrier for subsequent use, e.g., in tissue engineering. In this study, a one-step co-electrospray process was established for simultaneously encapsulating MSCs and gene with a vector in hydrogel microspheres, providing an environment for localized and high-efficiency gene delivery. Poly(ethylenimine)-poly(sorbate) (PEI-PS) conjugate was synthesized as vector and plasmid DNA encoding enhanced green fluorescence protein (pEGFP) was model gene. PEI-PS/pEGFP complexes at 5:1~15:1 were prepared. Co-electrospray of MSCs and PEI-PS/pEGFP complexes was conducted via coaxial electrospray: inner capillary of coaxial spinneret was fed by a MSC suspension with PEI-PS/pEGFP complexes and outer capillary was fed by a Na-alginate aqueous solution, which led to formation of hydrogel microspheres with PEI-PS/pEGFP complexes and MSCs in the liquid core. Various experiments were conducted subsequently. The gene transfection efficiency was found to be enhanced inside the microsphere environment. |
Description | PACRIM Poster Session (non-student) - no. PACRIM-P-126-2017 |
Persistent Identifier | http://hdl.handle.net/10722/244448 |
DC Field | Value | Language |
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dc.contributor.author | Zhao, Q | - |
dc.contributor.author | Sun, H | - |
dc.contributor.author | Wang, M | - |
dc.date.accessioned | 2017-09-18T01:52:38Z | - |
dc.date.available | 2017-09-18T01:52:38Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | 12th Pacific Rim Conference on Ceramic and Glass Technology (PACRIM 12), including Glass & Optical Materials Division Meeting (GOMD 2017), Waikoloa, Hawaii, USA, 21-26 May 2017. In Conference Program, p. 150 | - |
dc.identifier.uri | http://hdl.handle.net/10722/244448 | - |
dc.description | PACRIM Poster Session (non-student) - no. PACRIM-P-126-2017 | - |
dc.description.abstract | Stem cell-based therapies are promising for treating human tissue loss. Mesenchymal stem cells (MSCs) are increasingly used. After delivery to target, MSCs need to have a suitable environment for differentiation and their fate should be appropriately directed. One approach is to modify MSCs first via gene delivery and then encapsulate genetically engineered MSCs in a carrier for subsequent use, e.g., in tissue engineering. In this study, a one-step co-electrospray process was established for simultaneously encapsulating MSCs and gene with a vector in hydrogel microspheres, providing an environment for localized and high-efficiency gene delivery. Poly(ethylenimine)-poly(sorbate) (PEI-PS) conjugate was synthesized as vector and plasmid DNA encoding enhanced green fluorescence protein (pEGFP) was model gene. PEI-PS/pEGFP complexes at 5:1~15:1 were prepared. Co-electrospray of MSCs and PEI-PS/pEGFP complexes was conducted via coaxial electrospray: inner capillary of coaxial spinneret was fed by a MSC suspension with PEI-PS/pEGFP complexes and outer capillary was fed by a Na-alginate aqueous solution, which led to formation of hydrogel microspheres with PEI-PS/pEGFP complexes and MSCs in the liquid core. Various experiments were conducted subsequently. The gene transfection efficiency was found to be enhanced inside the microsphere environment. | - |
dc.language | eng | - |
dc.publisher | The American Ceramic Society. | - |
dc.relation.ispartof | Pacific Rim Conference on Ceramics and Glass Technology (PacRim12) | - |
dc.title | Co-electrospray of mesemchymal stem cells and gene with non-viral vector in hydrogel microspheres for enhanced stem cell-based therapy | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Wang, M: memwang@hku.hk | - |
dc.identifier.authority | Wang, M=rp00185 | - |
dc.identifier.hkuros | 278816 | - |
dc.identifier.spage | 150 | - |
dc.identifier.epage | 150 | - |
dc.publisher.place | Waikoloa, Hawaii, USA | - |