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Article: Asymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration

TitleAsymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration
Authors
Issue Date2017
PublisherNature Publishing Group: Nature Communications. The Journal's web site is located at http://www.nature.com/ncomms/index.html
Citation
Nature Communications, 2017, v. 8, article no. 1185 How to Cite?
AbstractFollowing epithelial-mesenchymal transition, acquisition of avian trunk neural crest cell (NCC) polarity is prerequisite for directional delamination and migration, which in turn is essential for peripheral nervous system development. However, how this cell polarization is established and regulated remains unknown. Here we demonstrate that, using the RHOA biosensor in vivo and in vitro, the initiation of NCC polarization is accompanied by highly activated RHOA in the cytoplasm at the cell rear and its fluctuating activity at the front edge. This differential RHOA activity determines polarized NC morphology and motility, and is regulated by the asymmetrically localized RhoGAP Deleted in liver cancer (DLC1) in the cytoplasm at the cell front. Importantly, the association of DLC1 with NEDD9 is crucial for its asymmetric localization and differential RHOA activity. Moreover, NC specifiers, SOX9 and SOX10, regulate NEDD9 and DLC1 expression, respectively. These results present a SOX9/SOX10-NEDD9/DLC1-RHOA regulatory axis to govern NCC migratory polarization.
Persistent Identifierhttp://hdl.handle.net/10722/244670
ISSN
2019 Impact Factor: 12.121
2015 SCImago Journal Rankings: 6.539
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLiu, AJ-
dc.contributor.authorRao, Y-
dc.contributor.authorCheung, MPL-
dc.contributor.authorHui, MN-
dc.contributor.authorWu, MH-
dc.contributor.authorChan, LK-
dc.contributor.authorNg, IOL-
dc.contributor.authorNiu, B-
dc.contributor.authorCheah, KSE-
dc.contributor.authorSharma, R-
dc.contributor.authorHodgson, L-
dc.contributor.authorCheung, MCH-
dc.date.accessioned2017-09-18T01:56:54Z-
dc.date.available2017-09-18T01:56:54Z-
dc.date.issued2017-
dc.identifier.citationNature Communications, 2017, v. 8, article no. 1185-
dc.identifier.issn2041-1723-
dc.identifier.urihttp://hdl.handle.net/10722/244670-
dc.description.abstractFollowing epithelial-mesenchymal transition, acquisition of avian trunk neural crest cell (NCC) polarity is prerequisite for directional delamination and migration, which in turn is essential for peripheral nervous system development. However, how this cell polarization is established and regulated remains unknown. Here we demonstrate that, using the RHOA biosensor in vivo and in vitro, the initiation of NCC polarization is accompanied by highly activated RHOA in the cytoplasm at the cell rear and its fluctuating activity at the front edge. This differential RHOA activity determines polarized NC morphology and motility, and is regulated by the asymmetrically localized RhoGAP Deleted in liver cancer (DLC1) in the cytoplasm at the cell front. Importantly, the association of DLC1 with NEDD9 is crucial for its asymmetric localization and differential RHOA activity. Moreover, NC specifiers, SOX9 and SOX10, regulate NEDD9 and DLC1 expression, respectively. These results present a SOX9/SOX10-NEDD9/DLC1-RHOA regulatory axis to govern NCC migratory polarization.-
dc.languageeng-
dc.publisherNature Publishing Group: Nature Communications. The Journal's web site is located at http://www.nature.com/ncomms/index.html-
dc.relation.ispartofNature Communications-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleAsymmetric localization of DLC1 defines avian trunk neural crest polarity for directional delamination and migration-
dc.typeArticle-
dc.identifier.emailLiu, AJ: jessie11@hku.hk-
dc.identifier.emailCheung, MPL: mplcheun@hku.hk-
dc.identifier.emailHui, MN: mnhui@hku.hk-
dc.identifier.emailWu, MH: ronmhwu@hkucc.hku.hk-
dc.identifier.emailChan, LK: lkchan1@hku.hk-
dc.identifier.emailNg, IOL: iolng@hku.hk-
dc.identifier.emailNiu, B: csniuben@hku.hk-
dc.identifier.emailCheah, KSE: hrmbdkc@hku.hk-
dc.identifier.emailCheung, MCH: mcheung9@hku.hk-
dc.identifier.authorityLiu, AJ=rp02546-
dc.identifier.authorityChan, LK=rp02289-
dc.identifier.authorityNg, IOL=rp00335-
dc.identifier.authorityCheah, KSE=rp00342-
dc.identifier.authorityCheung, MCH=rp00245-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/s41467-017-01107-0-
dc.identifier.pmid29084958-
dc.identifier.pmcidPMC5662599-
dc.identifier.scopuseid_2-s2.0-85032572965-
dc.identifier.hkuros277871-
dc.identifier.hkuros276243-
dc.identifier.hkuros286509-
dc.identifier.volume8-
dc.identifier.spagearticle no. 1185-
dc.identifier.epagearticle no. 1185-
dc.identifier.isiWOS:000413894100007-
dc.publisher.placeUnited Kingdom-

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