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Conference Paper: Impact of systemic inflammation on neuroinflammation, cognitive functions and phosphorylation of tau by laparotomy. Implication of postoperative cognitive dysfunctions to Alzheimer’s dementia

TitleImpact of systemic inflammation on neuroinflammation, cognitive functions and phosphorylation of tau by laparotomy. Implication of postoperative cognitive dysfunctions to Alzheimer’s dementia
Authors
Issue Date2017
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/NDD
Citation
Abstracts of the 13th International Conference on Alzheimer’s & Parkinson’s Diseases, Vienna, Austria, 29 March-2 April 2017. In Neurodegenerative Diseases, 2017, v. 17 n. Suppl. 1, p. 1167 How to Cite?
AbstractAims: Systemic inflammation triggered by infection or surgery can stimulate neuroinflammation, and thereby disturbing cognitive functions and even developing Alzheimer’s dementia. We have recently adopted laparotomy surgery as an experimental model for postoperative cognitive dysfunctions (POCD). We aimed to investigate the temporal profile of systemic inflammation, neuroinflammation and cognitive functions for 2 weeks, which has high implication in the development of Alzheimer’s dementia. Method: Adult male wild type C57BL/6N mice (3-month-old) were used for 2 series of experiments. Mice were divided into 3 groups: Control (CON), sevoflurane only (SEVO) and Laparotomy under (LAP). Cognitive function was assessed by Y-maze and Novel Objective Recognition test (NOR). Inflammatory cytokine mRNA expression in liver, frontal cortex and hippocampus were assessed by q-PCR; protein levels in brain tissues and plasma were determined by MILIPLEX assay. Results: There were significantly greater number of errors and longer latency in LAP compared with SEVO in Y-maze test; and higher discrimination index in NOR. Neuroinflammation was found by an increase in IL-1β and IL-6 in the frontal cortex, and IL-1β and IL-8 in the hippocampus. Immunoreactivity of GFAP-labeled astrocytes was higher in LAP in the frontal cortex and activated microglia (Iba1 immunoreactivity) were found in both brain regions. Increased phosphorylation of tau was detected in both brain regions in LAP at 14d. Neuro- and peripheral inflammation and tau protein phosphorylation were reversed by ibuprofen, and cognition deficits were improved as well. Conclusion: Neuroinflammation induced by laparotomy activates astrocytes and microglia, increases tau phosphorylation that finally impaired the cognition.
DescriptionB01.c. Disease Mechanisms, Pathophysiology: Inflammation - no. ADPD7-0595
Persistent Identifierhttp://hdl.handle.net/10722/248340
ISSN
2019 Impact Factor: 2.418
2015 SCImago Journal Rankings: 1.472

 

DC FieldValueLanguage
dc.contributor.authorChang, RCC-
dc.contributor.authorHuang, C-
dc.contributor.authorWong, GTC-
dc.date.accessioned2017-10-18T08:41:39Z-
dc.date.available2017-10-18T08:41:39Z-
dc.date.issued2017-
dc.identifier.citationAbstracts of the 13th International Conference on Alzheimer’s & Parkinson’s Diseases, Vienna, Austria, 29 March-2 April 2017. In Neurodegenerative Diseases, 2017, v. 17 n. Suppl. 1, p. 1167-
dc.identifier.issn1660-2854-
dc.identifier.urihttp://hdl.handle.net/10722/248340-
dc.descriptionB01.c. Disease Mechanisms, Pathophysiology: Inflammation - no. ADPD7-0595-
dc.description.abstractAims: Systemic inflammation triggered by infection or surgery can stimulate neuroinflammation, and thereby disturbing cognitive functions and even developing Alzheimer’s dementia. We have recently adopted laparotomy surgery as an experimental model for postoperative cognitive dysfunctions (POCD). We aimed to investigate the temporal profile of systemic inflammation, neuroinflammation and cognitive functions for 2 weeks, which has high implication in the development of Alzheimer’s dementia. Method: Adult male wild type C57BL/6N mice (3-month-old) were used for 2 series of experiments. Mice were divided into 3 groups: Control (CON), sevoflurane only (SEVO) and Laparotomy under (LAP). Cognitive function was assessed by Y-maze and Novel Objective Recognition test (NOR). Inflammatory cytokine mRNA expression in liver, frontal cortex and hippocampus were assessed by q-PCR; protein levels in brain tissues and plasma were determined by MILIPLEX assay. Results: There were significantly greater number of errors and longer latency in LAP compared with SEVO in Y-maze test; and higher discrimination index in NOR. Neuroinflammation was found by an increase in IL-1β and IL-6 in the frontal cortex, and IL-1β and IL-8 in the hippocampus. Immunoreactivity of GFAP-labeled astrocytes was higher in LAP in the frontal cortex and activated microglia (Iba1 immunoreactivity) were found in both brain regions. Increased phosphorylation of tau was detected in both brain regions in LAP at 14d. Neuro- and peripheral inflammation and tau protein phosphorylation were reversed by ibuprofen, and cognition deficits were improved as well. Conclusion: Neuroinflammation induced by laparotomy activates astrocytes and microglia, increases tau phosphorylation that finally impaired the cognition.-
dc.languageeng-
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/NDD-
dc.relation.ispartofNeurodegenerative Diseases-
dc.rightsNeurodegenerative Diseases. Copyright © S Karger AG.-
dc.titleImpact of systemic inflammation on neuroinflammation, cognitive functions and phosphorylation of tau by laparotomy. Implication of postoperative cognitive dysfunctions to Alzheimer’s dementia-
dc.typeConference_Paper-
dc.identifier.emailChang, RCC: rccchang@hku.hk-
dc.identifier.emailWong, GTC: gordon@hku.hk-
dc.identifier.authorityChang, RCC=rp00470-
dc.identifier.authorityWong, GTC=rp00523-
dc.identifier.hkuros280640-
dc.identifier.volume17-
dc.identifier.issueSuppl. 1-
dc.identifier.spage1167-
dc.identifier.epage1167-
dc.publisher.placeSwitzerland-

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