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Conference Paper: A dox-terminable cell-encapsulating device for intravitreal drug delivery

TitleA dox-terminable cell-encapsulating device for intravitreal drug delivery
Authors
Tsang, KKWong, FSYYao, KMChan, BPLai, JSMShih, KCLo, ACY
Issue Date2016
Citation
28th Annual Scientific Meeting of Hong Kong Ophthalmological Symposium: Ophthalmic Diagnostics and Lasers, Hong Kong, 19-20 November 2016 How to Cite?
AbstractObjectives: Encapsulated-cell therapy presents a way of localized and sustained drug delivery to the posterior eye. An injectable collagen-alginate drug delivery platform was designed with promising result previously. Now, a Tet-On inducible apoptotic control is further introduced for safety. Methods: GDNF-expression HEK293 cells were transfected with pcDNATM6/TR and recombinant Caspase 8 plasmid. Cell death upon Dox exposure in cell-encapsulating collagen-alginate gel was assayed by MTS. Biocompatibility and stability of the gel system as well as its encapsulated cell viability were evaluated on 7 and 28 days post intravitreal injection by microscopy. Results & conclusion: Complete cell death via caspase-mediated apoptosis was achievable within 72 hours of 0-2ug/ml Dox induction. Intravitreally injected device displayed good biocompatibility, mechanical stability and encapsulated cell viability after prolonged implantation in RCS rat eyes. We have successfully established a Dox-terminable cell-encapsulating intravitreal drug delivery device with good biocompatibility and stability after implantation in rats.
DescriptionThe Symposium was jointly organized by the Hong Kong Ophthalmological Society and the College of Ophthalmologists of Hong Kong
Poster Presentation
Persistent Identifierhttp://hdl.handle.net/10722/249513

 

DC FieldValueLanguage
dc.contributor.authorTsang, KK-
dc.contributor.authorWong, FSY-
dc.contributor.authorYao, KM-
dc.contributor.authorChan, BP-
dc.contributor.authorLai, JSM-
dc.contributor.authorShih, KC-
dc.contributor.authorLo, ACY-
dc.date.accessioned2017-11-21T03:03:17Z-
dc.date.available2017-11-21T03:03:17Z-
dc.date.issued2016-
dc.identifier.citation28th Annual Scientific Meeting of Hong Kong Ophthalmological Symposium: Ophthalmic Diagnostics and Lasers, Hong Kong, 19-20 November 2016-
dc.identifier.urihttp://hdl.handle.net/10722/249513-
dc.descriptionThe Symposium was jointly organized by the Hong Kong Ophthalmological Society and the College of Ophthalmologists of Hong Kong-
dc.descriptionPoster Presentation-
dc.description.abstractObjectives: Encapsulated-cell therapy presents a way of localized and sustained drug delivery to the posterior eye. An injectable collagen-alginate drug delivery platform was designed with promising result previously. Now, a Tet-On inducible apoptotic control is further introduced for safety. Methods: GDNF-expression HEK293 cells were transfected with pcDNATM6/TR and recombinant Caspase 8 plasmid. Cell death upon Dox exposure in cell-encapsulating collagen-alginate gel was assayed by MTS. Biocompatibility and stability of the gel system as well as its encapsulated cell viability were evaluated on 7 and 28 days post intravitreal injection by microscopy. Results & conclusion: Complete cell death via caspase-mediated apoptosis was achievable within 72 hours of 0-2ug/ml Dox induction. Intravitreally injected device displayed good biocompatibility, mechanical stability and encapsulated cell viability after prolonged implantation in RCS rat eyes. We have successfully established a Dox-terminable cell-encapsulating intravitreal drug delivery device with good biocompatibility and stability after implantation in rats.-
dc.languageeng-
dc.relation.ispartofHong Kong Ophthalmological Symposium 2016-
dc.titleA dox-terminable cell-encapsulating device for intravitreal drug delivery-
dc.typeConference_Paper-
dc.identifier.emailWong, FSY: frann@hku.hk-
dc.identifier.emailYao, KM: kmyao@hku.hk-
dc.identifier.emailChan, BP: bpchan@hku.hk-
dc.identifier.emailLai, JSM: laism@hku.hk-
dc.identifier.emailShih, KC: kcshih@hku.hk-
dc.identifier.emailLo, ACY: amylo@hku.hk-
dc.identifier.authorityYao, KM=rp00344-
dc.identifier.authorityChan, BP=rp00087-
dc.identifier.authorityLai, JSM=rp00295-
dc.identifier.authorityShih, KC=rp01374-
dc.identifier.authorityLo, ACY=rp00425-
dc.identifier.hkuros282657-

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