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Conference Paper: Advanced combinatorial genetics technology for decoding biological complexity
Title | Advanced combinatorial genetics technology for decoding biological complexity |
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Authors | |
Issue Date | 2017 |
Citation | Seminar, Department of Biomedical Science, City University of Hong Kong, Hong Kong, 14 August 2017 How to Cite? |
Abstract | The orchestrated action of genes controls complex biological traits, yet the systematic discovery of gene combinations that modulate these phenotypes in human cells is labor-intensive and challenging to scale. Harnessing the power of synthetic biology and next-generation sequencing technologies, we developed CombiGEM (Combinatorial Genetics En Masse) as a powerful platform for high-throughput functional characterization of combinatorial genetic perturbations in human cells. CombiGEM enables rapid, scalable assembly of high-order barcoded combinatorial genetic libraries, and multiplexed quantification of all library members by using next-generation sequencing technologies. The genetic elements included in CombiGEM libraries can be arbitrary, including microRNAs, gene expression/knockdown constructs, and programmable CRISPR-Cas genome editing tools. We envision that this technology platform will be applicable to a broad range of biological settings, and will enable the systematic identification of genetic combinations that regulate complex biological processes and diseases. |
Persistent Identifier | http://hdl.handle.net/10722/252921 |
DC Field | Value | Language |
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dc.contributor.author | Wong, SL | - |
dc.date.accessioned | 2018-05-09T02:35:02Z | - |
dc.date.available | 2018-05-09T02:35:02Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Seminar, Department of Biomedical Science, City University of Hong Kong, Hong Kong, 14 August 2017 | - |
dc.identifier.uri | http://hdl.handle.net/10722/252921 | - |
dc.description.abstract | The orchestrated action of genes controls complex biological traits, yet the systematic discovery of gene combinations that modulate these phenotypes in human cells is labor-intensive and challenging to scale. Harnessing the power of synthetic biology and next-generation sequencing technologies, we developed CombiGEM (Combinatorial Genetics En Masse) as a powerful platform for high-throughput functional characterization of combinatorial genetic perturbations in human cells. CombiGEM enables rapid, scalable assembly of high-order barcoded combinatorial genetic libraries, and multiplexed quantification of all library members by using next-generation sequencing technologies. The genetic elements included in CombiGEM libraries can be arbitrary, including microRNAs, gene expression/knockdown constructs, and programmable CRISPR-Cas genome editing tools. We envision that this technology platform will be applicable to a broad range of biological settings, and will enable the systematic identification of genetic combinations that regulate complex biological processes and diseases. | - |
dc.language | eng | - |
dc.relation.ispartof | City University of Hong Kong, Department of Biomedical Science, Seminar | - |
dc.title | Advanced combinatorial genetics technology for decoding biological complexity | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Wong, SL: aslw@hku.hk | - |
dc.identifier.authority | Wong, SL=rp02139 | - |
dc.identifier.hkuros | 280989 | - |