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Book Chapter: Derivation of Fate-Committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats

TitleDerivation of Fate-Committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats
Authors
KeywordsBMSC-derived Schwann cells
Bone marrow stromal cells
Cell-based therapy
Myelination
Nerve regeneration
Issue Date2018
PublisherHumana Press.
Citation
Derivation of Fate-Committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats. In Monje, PV and Kim, HA (Eds.), Schwann Cells: Methods and Protocols, p. 137-148. New York, NY: Humana Press, 2018 How to Cite?
AbstractOur goal is to derive phenotypically stable Schwann cells from bone marrow stromal cells (BMSCs) for use in transplantation studies of central/peripheral nerve injuries. With the adult rat as model, here we describe steps that foster (1) expansion of the BMSC subpopulation of neural progenitors as neurosphere cells, (2) differentiation of the progenitors into Schwann cell-like cells in adherent culture supplemented with soluble factors, and (3) cell-intrinsic switch of Schwann cell-like cells to the Schwann cell fate following co-culture with sensory neurons purified from dorsal root ganglia. The derived Schwann cells retain marker expression despite withdrawal of supplements and neuronal cues, survive passaging and cryopreservation, and, importantly, show functional capacity for myelination.
Persistent Identifierhttp://hdl.handle.net/10722/259091
ISBN
ISSN
2015 SCImago Journal Rankings: 0.549
Series/Report no.Methods in Molecular Biology; 1739

 

DC FieldValueLanguage
dc.contributor.authorTsui, YP-
dc.contributor.authorShea, GKH-
dc.contributor.authorChan, YS-
dc.contributor.authorShum, DKY-
dc.date.accessioned2018-09-03T04:01:21Z-
dc.date.available2018-09-03T04:01:21Z-
dc.date.issued2018-
dc.identifier.citationDerivation of Fate-Committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats. In Monje, PV and Kim, HA (Eds.), Schwann Cells: Methods and Protocols, p. 137-148. New York, NY: Humana Press, 2018-
dc.identifier.isbn9781493976485-
dc.identifier.issn1064-3745-
dc.identifier.urihttp://hdl.handle.net/10722/259091-
dc.description.abstractOur goal is to derive phenotypically stable Schwann cells from bone marrow stromal cells (BMSCs) for use in transplantation studies of central/peripheral nerve injuries. With the adult rat as model, here we describe steps that foster (1) expansion of the BMSC subpopulation of neural progenitors as neurosphere cells, (2) differentiation of the progenitors into Schwann cell-like cells in adherent culture supplemented with soluble factors, and (3) cell-intrinsic switch of Schwann cell-like cells to the Schwann cell fate following co-culture with sensory neurons purified from dorsal root ganglia. The derived Schwann cells retain marker expression despite withdrawal of supplements and neuronal cues, survive passaging and cryopreservation, and, importantly, show functional capacity for myelination.-
dc.languageeng-
dc.publisherHumana Press.-
dc.relation.ispartofSchwann Cells: Methods and Protocols-
dc.relation.ispartofseriesMethods in Molecular Biology; 1739-
dc.subjectBMSC-derived Schwann cells-
dc.subjectBone marrow stromal cells-
dc.subjectCell-based therapy-
dc.subjectMyelination-
dc.subjectNerve regeneration-
dc.titleDerivation of Fate-Committed Schwann Cells from Bone Marrow Stromal Cells of Adult Rats-
dc.typeBook_Chapter-
dc.identifier.emailTsui, YP: alex2013@HKUCC-COM.hku.hk-
dc.identifier.emailShea, GKH: gkshea@hku.hk-
dc.identifier.emailChan, YS: yschan@hku.hk-
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hk-
dc.identifier.authorityShea, GKH=rp01781-
dc.identifier.authorityChan, YS=rp00318-
dc.identifier.authorityShum, DKY=rp00321-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/978-1-4939-7649-2_9-
dc.identifier.pmid29546705-
dc.identifier.scopuseid_2-s2.0-85044175057-
dc.identifier.hkuros288461-
dc.identifier.hkuros291235-
dc.identifier.spage137-
dc.identifier.epage148-
dc.publisher.placeNew York, NY-

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