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Article: Preparation and characterization of SDF-1α-chitosan-dextran sulfate nanoparticles

TitlePreparation and characterization of SDF-1α-chitosan-dextran sulfate nanoparticles
Authors
Issue Date2015
PublisherJournal of Visualized Experiments. The Journal's web site is located at http://www.jove.com
Citation
Journal of Visualized Experiments, 2015, n. 95, p. 52323 How to Cite?
AbstractChitosan (CS) and dextran sulfate (DS) are charged polysaccharides (glycans), which form polyelectrolyte complex-based nanoparticles when mixed under appropriate conditions. The glycan nanoparticles are useful carriers for protein factors, which facilitate the in vivo delivery of the proteins and sustain their retention in the targeted tissue. The glycan polyelectrolyte complexes are also ideal for protein delivery, as the incorporation is carried out in aqueous solution, which reduces the likelihood of inactivation of the proteins. Proteins with a heparin-binding site adhere to dextran sulfate readily, and are, in turn, stabilized by the binding. These particles are also less inflammatory and toxic when delivered in vivo. In the protocol described below, SDF-1α (Stromal cell-derived factor-1α), a stem cell homing factor, is first mixed and incubated with dextran sulfate. Chitosan is added to the mixture to form polyelectrolyte complexes, followed by zinc sulfate to stabilize the complexes with zinc bridges. The resultant SDF-1α-DS-CS particles are measured for size (diameter) and surface charge (zeta potential). The amount of the incorporated SDF-1α is determined, followed by measurements of its in vitro release rate and its chemotactic activity in a particle-bound form.
Persistent Identifierhttp://hdl.handle.net/10722/260276
ISSN
2017 Impact Factor: 1.184
2015 SCImago Journal Rankings: 0.451
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorBader, AR-
dc.contributor.authorLi, T-
dc.contributor.authorWang, W-
dc.contributor.authorKohane, DS-
dc.contributor.authorLoscalzo, J-
dc.contributor.authorZhang, YY-
dc.date.accessioned2018-09-12T06:52:23Z-
dc.date.available2018-09-12T06:52:23Z-
dc.date.issued2015-
dc.identifier.citationJournal of Visualized Experiments, 2015, n. 95, p. 52323-
dc.identifier.issn1940-087X-
dc.identifier.urihttp://hdl.handle.net/10722/260276-
dc.description.abstractChitosan (CS) and dextran sulfate (DS) are charged polysaccharides (glycans), which form polyelectrolyte complex-based nanoparticles when mixed under appropriate conditions. The glycan nanoparticles are useful carriers for protein factors, which facilitate the in vivo delivery of the proteins and sustain their retention in the targeted tissue. The glycan polyelectrolyte complexes are also ideal for protein delivery, as the incorporation is carried out in aqueous solution, which reduces the likelihood of inactivation of the proteins. Proteins with a heparin-binding site adhere to dextran sulfate readily, and are, in turn, stabilized by the binding. These particles are also less inflammatory and toxic when delivered in vivo. In the protocol described below, SDF-1α (Stromal cell-derived factor-1α), a stem cell homing factor, is first mixed and incubated with dextran sulfate. Chitosan is added to the mixture to form polyelectrolyte complexes, followed by zinc sulfate to stabilize the complexes with zinc bridges. The resultant SDF-1α-DS-CS particles are measured for size (diameter) and surface charge (zeta potential). The amount of the incorporated SDF-1α is determined, followed by measurements of its in vitro release rate and its chemotactic activity in a particle-bound form.-
dc.languageeng-
dc.publisherJournal of Visualized Experiments. The Journal's web site is located at http://www.jove.com-
dc.relation.ispartofJournal of Visualized Experiments-
dc.rightsJournal of Visualized Experiments. Copyright © Journal of Visualized Experiments.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titlePreparation and characterization of SDF-1α-chitosan-dextran sulfate nanoparticles-
dc.typeArticle-
dc.identifier.emailWang, W: wangwp@hku.hk-
dc.identifier.authorityWang, W=rp02227-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3791/52323-
dc.identifier.pmid25650558-
dc.identifier.pmcidPMC4354548-
dc.identifier.hkuros700003528-
dc.identifier.issue95-
dc.identifier.spage52323-
dc.identifier.epage52323-
dc.identifier.isiWOS:000361532900053-
dc.publisher.placeUnited States-

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