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Article: Down‐regulation of TROP‐2 Predicts Poor Prognosis of Hepatocellular Carcinoma Patients

TitleDown‐regulation of TROP‐2 Predicts Poor Prognosis of Hepatocellular Carcinoma Patients
Authors
Issue Date2018
PublisherJohn Wiley & Sons Ltd.. The Journal's web site is located at http://aasldpubs.onlinelibrary.wiley.com/hub/journal/10.1002/(ISSN)2471-254X/
Citation
Hepatology Communications, 2018, v. 2 n. 11, p. 1408-1414 How to Cite?
AbstractHepatocellular carcinoma (HCC) is one of the most common and lethal cancer types worldwide, especially in Asian countries. Genetic alterations, including hyperactivation of oncogenes and loss of expression of tumor suppressor genes, greatly contribute to the initiation and progression of HCC. Here we report that down‐regulation of trophoblast cell surface antigen 2 (TROP‐2) was frequently detected in HCC. Transcriptome sequencing of non‐tumor and HCC patient samples revealed down‐regulation of TROP‐2 in tumor tissues. Immunohistochemical staining showed nearly undetectable levels of TROP‐2 in HCC tissues but distinct and strong staining of TROP‐2 in adjacent non‐tumor tissues. The frequent down‐regulation of TROP‐2 expression was further confirmed in an in‐house cohort of 205 pairs of HCC patient samples and in the Cancer Genome Atlas (TCGA) databases. Furthermore, the down‐regulation of TROP‐2 was associated with poor overall survival of HCC patients, severe adjacent organ invasion, and poor differentiation of HCC. Using bisulfite genomic sequencing and methylation‐specific polymerase chain reaction analyses, we show that higher levels of promoter methylation were detected in the DNA samples of HCC tissues (low TROP‐2 expression) than that of the non‐tumor tissues (high TROP‐2 expression). Conclusion: Taken together, our data suggest that promoter hypermethylation contributes to the frequent down‐regulation of TROP‐2 in HCC, and that TROP‐2 down‐regulation predicts poor prognosis of HCC patients.
Persistent Identifierhttp://hdl.handle.net/10722/262278
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSin, ST-
dc.contributor.authorLi, Y-
dc.contributor.authorLiu, M-
dc.contributor.authorYuan, YF-
dc.contributor.authorMa, SKY-
dc.contributor.authorGuan, X-
dc.date.accessioned2018-09-28T04:56:30Z-
dc.date.available2018-09-28T04:56:30Z-
dc.date.issued2018-
dc.identifier.citationHepatology Communications, 2018, v. 2 n. 11, p. 1408-1414-
dc.identifier.issn2471-254X-
dc.identifier.urihttp://hdl.handle.net/10722/262278-
dc.description.abstractHepatocellular carcinoma (HCC) is one of the most common and lethal cancer types worldwide, especially in Asian countries. Genetic alterations, including hyperactivation of oncogenes and loss of expression of tumor suppressor genes, greatly contribute to the initiation and progression of HCC. Here we report that down‐regulation of trophoblast cell surface antigen 2 (TROP‐2) was frequently detected in HCC. Transcriptome sequencing of non‐tumor and HCC patient samples revealed down‐regulation of TROP‐2 in tumor tissues. Immunohistochemical staining showed nearly undetectable levels of TROP‐2 in HCC tissues but distinct and strong staining of TROP‐2 in adjacent non‐tumor tissues. The frequent down‐regulation of TROP‐2 expression was further confirmed in an in‐house cohort of 205 pairs of HCC patient samples and in the Cancer Genome Atlas (TCGA) databases. Furthermore, the down‐regulation of TROP‐2 was associated with poor overall survival of HCC patients, severe adjacent organ invasion, and poor differentiation of HCC. Using bisulfite genomic sequencing and methylation‐specific polymerase chain reaction analyses, we show that higher levels of promoter methylation were detected in the DNA samples of HCC tissues (low TROP‐2 expression) than that of the non‐tumor tissues (high TROP‐2 expression). Conclusion: Taken together, our data suggest that promoter hypermethylation contributes to the frequent down‐regulation of TROP‐2 in HCC, and that TROP‐2 down‐regulation predicts poor prognosis of HCC patients.-
dc.languageeng-
dc.publisherJohn Wiley & Sons Ltd.. The Journal's web site is located at http://aasldpubs.onlinelibrary.wiley.com/hub/journal/10.1002/(ISSN)2471-254X/-
dc.relation.ispartofHepatology Communications-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleDown‐regulation of TROP‐2 Predicts Poor Prognosis of Hepatocellular Carcinoma Patients-
dc.typeArticle-
dc.identifier.emailMa, SKY: stefma@hku.hk-
dc.identifier.emailGuan, X: xyguan@hku.hk-
dc.identifier.authorityMa, SKY=rp00506-
dc.identifier.authorityGuan, X=rp00454-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1002/hep4.1242-
dc.identifier.hkuros292983-
dc.identifier.volume2-
dc.identifier.issue11-
dc.identifier.spage1408-
dc.identifier.epage1414-
dc.identifier.isiWOS:000452466300013-
dc.publisher.placeUnited Kingdom-
dc.identifier.issnl2471-254X-

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