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Article: ATP compartmentation in plastids and cytosol of Arabidopsis thaliana revealed by fluorescent protein sensing

TitleATP compartmentation in plastids and cytosol of Arabidopsis thaliana revealed by fluorescent protein sensing
Authors
KeywordsATP
Chloroplasts
Cytosol
Mitochondria
Photosynthesis
Issue Date2018
PublisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org
Citation
Proceedings of the National Academy of Sciences, 2018, v. 115 n. 45, p. E10778-E10787 How to Cite?
AbstractMatching ATP:NADPH provision and consumption in the chloroplast is a prerequisite for efficient photosynthesis. In terms of ATP: NADPH ratio, the amount of ATP generated from the linear electron flow does not meet the demand of the Calvin-Benson- Bassham (CBB) cycle. Several different mechanisms to increase ATP availability have evolved, including cyclic electron flow in higher plants and the direct import of mitochondrial-derived ATP in diatoms. By imaging a fluorescent ATP sensor protein expressed in living Arabidopsis thaliana seedlings, we found that MgATP2- concentrations were lower in the stroma of mature chloroplasts than in the cytosol, and exogenous ATP was able to enter chloroplasts isolated from 4- and 5-day-old seedlings, but not chloroplasts isolated from 10- or 20-day-old photosynthetic tissues. This observation is in line with the previous finding that the expression of chloroplast nucleotide transporters (NTTs) in Arabidopsis mesophyll is limited to very young seedlings. Employing a combination of photosynthetic and respiratory inhibitors with compartment-specific imaging of ATP, we corroborate the dependency of stromal ATP production on mitochondrial dissipation of photosynthetic reductant. Our data suggest that, during illumination, the provision and consumption of ATP:NADPH in chloroplasts can be balanced by exporting excess reductants rather than importing ATP from the cytosol.
Persistent Identifierhttp://hdl.handle.net/10722/266076
ISSN
2017 Impact Factor: 9.504
2015 SCImago Journal Rankings: 6.883
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorVoon, CP-
dc.contributor.authorGuan, X-
dc.contributor.authorSun, Y-
dc.contributor.authorSahu, A-
dc.contributor.authorChan, MN-
dc.contributor.authorGardeström, P-
dc.contributor.authorWagner, S-
dc.contributor.authorFuchs, P-
dc.contributor.authorNietzel, T-
dc.contributor.authorVersaw, WK-
dc.contributor.authorSchwarzländer, M-
dc.contributor.authorLim, BL-
dc.date.accessioned2018-12-17T02:16:43Z-
dc.date.available2018-12-17T02:16:43Z-
dc.date.issued2018-
dc.identifier.citationProceedings of the National Academy of Sciences, 2018, v. 115 n. 45, p. E10778-E10787-
dc.identifier.issn0027-8424-
dc.identifier.urihttp://hdl.handle.net/10722/266076-
dc.description.abstractMatching ATP:NADPH provision and consumption in the chloroplast is a prerequisite for efficient photosynthesis. In terms of ATP: NADPH ratio, the amount of ATP generated from the linear electron flow does not meet the demand of the Calvin-Benson- Bassham (CBB) cycle. Several different mechanisms to increase ATP availability have evolved, including cyclic electron flow in higher plants and the direct import of mitochondrial-derived ATP in diatoms. By imaging a fluorescent ATP sensor protein expressed in living Arabidopsis thaliana seedlings, we found that MgATP2- concentrations were lower in the stroma of mature chloroplasts than in the cytosol, and exogenous ATP was able to enter chloroplasts isolated from 4- and 5-day-old seedlings, but not chloroplasts isolated from 10- or 20-day-old photosynthetic tissues. This observation is in line with the previous finding that the expression of chloroplast nucleotide transporters (NTTs) in Arabidopsis mesophyll is limited to very young seedlings. Employing a combination of photosynthetic and respiratory inhibitors with compartment-specific imaging of ATP, we corroborate the dependency of stromal ATP production on mitochondrial dissipation of photosynthetic reductant. Our data suggest that, during illumination, the provision and consumption of ATP:NADPH in chloroplasts can be balanced by exporting excess reductants rather than importing ATP from the cytosol.-
dc.languageeng-
dc.publisherNational Academy of Sciences. The Journal's web site is located at http://www.pnas.org-
dc.relation.ispartofProceedings of the National Academy of Sciences-
dc.rightsProceedings of the National Academy of Sciences. Copyright © National Academy of Sciences.-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectATP-
dc.subjectChloroplasts-
dc.subjectCytosol-
dc.subjectMitochondria-
dc.subjectPhotosynthesis-
dc.titleATP compartmentation in plastids and cytosol of Arabidopsis thaliana revealed by fluorescent protein sensing-
dc.typeArticle-
dc.identifier.emailChan, MN: mnchan@hku.hk-
dc.identifier.emailLim, BL: bllim@hku.hk-
dc.identifier.authorityLim, BL=rp00744-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1073/pnas.1711497115-
dc.identifier.pmid30352850-
dc.identifier.pmcidPMC6233094-
dc.identifier.scopuseid_2-s2.0-85056138743-
dc.identifier.hkuros296400-
dc.identifier.volume115-
dc.identifier.issue45-
dc.identifier.spageE10778-
dc.identifier.epageE10787-
dc.identifier.isiWOS:000449459000031-
dc.publisher.placeUnited States-
dc.identifier.f1000734276016-

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