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Conference Paper: TUMOR SUPPRESSIVE ROLE OF MIR-199A-5P IN TRIPLE-NEGATIVE BREAST CANCER
Title | TUMOR SUPPRESSIVE ROLE OF MIR-199A-5P IN TRIPLE-NEGATIVE BREAST CANCER |
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Authors | |
Issue Date | 2016 |
Publisher | Korea Breast Cancer Society. |
Citation | The Global Breast Cancer Conference (GBCC), Jeju Island, Korea, 28-30 April 2016. In GBCC 2016 Abstract Book, p. 258 How to Cite? |
Abstract | Background: Triple-negative breast cancer (TNBC) is defined by lack of expression in estrogen, progesterone receptors and human epidermal growth factor receptor 2. TNBC exhibited a more aggressive phenotype than other subtypes. Our previous study identified miR-199a-5p as TNBC-specific circulating marker, so we further investigated its role in breast cancer. Methods: MTT, migration/invasion assay, cell cycle analysis, single-cell clonogenic assay and aldehyde dehydrogenase (ALDH) activity were performed in TNBC cell line MDA-MB-231 transfected with miR-199a-5p mimic. Gene expression level was investigated by real-time PCR. Results: Overexpression of miR-199a-5p significantly inhibited cell proliferation, migration and invasion leading to G0/G1 phase arrest, early apoptosis and alteration in epithelial-mesenchymal transition-related genes in MDA-MB-231. Also, miR-199a-5p correlated with stemness characteristics by inhibiting single cell colony formation and decreasing CD24-/CD44+ subpopulation and ALDH activity. ALDH1A3 had a higher expression in breast cancer plasma especially in TNBC when compared to normal individuals. Furthermore, luciferase assay identified that PIK3CD is the potential downstream target of miR-199a-5p. Conclusions: Our data implicated that miR-199a-5p confers tumor suppressive role in TNBC, which inhibited stemness characteristics. These findings may help the development of novel therapeutic strategies towards this highly malignant disease. |
Description | Poster Presentation - Tumor and Cell Biology - no. PO124 |
Persistent Identifier | http://hdl.handle.net/10722/268344 |
DC Field | Value | Language |
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dc.contributor.author | Chen, J | - |
dc.contributor.author | Shin, VY | - |
dc.contributor.author | Siu, JMT | - |
dc.contributor.author | Ho, CWJ | - |
dc.contributor.author | Cheuk, WYI | - |
dc.contributor.author | Kwong, A | - |
dc.date.accessioned | 2019-03-18T04:23:38Z | - |
dc.date.available | 2019-03-18T04:23:38Z | - |
dc.date.issued | 2016 | - |
dc.identifier.citation | The Global Breast Cancer Conference (GBCC), Jeju Island, Korea, 28-30 April 2016. In GBCC 2016 Abstract Book, p. 258 | - |
dc.identifier.uri | http://hdl.handle.net/10722/268344 | - |
dc.description | Poster Presentation - Tumor and Cell Biology - no. PO124 | - |
dc.description.abstract | Background: Triple-negative breast cancer (TNBC) is defined by lack of expression in estrogen, progesterone receptors and human epidermal growth factor receptor 2. TNBC exhibited a more aggressive phenotype than other subtypes. Our previous study identified miR-199a-5p as TNBC-specific circulating marker, so we further investigated its role in breast cancer. Methods: MTT, migration/invasion assay, cell cycle analysis, single-cell clonogenic assay and aldehyde dehydrogenase (ALDH) activity were performed in TNBC cell line MDA-MB-231 transfected with miR-199a-5p mimic. Gene expression level was investigated by real-time PCR. Results: Overexpression of miR-199a-5p significantly inhibited cell proliferation, migration and invasion leading to G0/G1 phase arrest, early apoptosis and alteration in epithelial-mesenchymal transition-related genes in MDA-MB-231. Also, miR-199a-5p correlated with stemness characteristics by inhibiting single cell colony formation and decreasing CD24-/CD44+ subpopulation and ALDH activity. ALDH1A3 had a higher expression in breast cancer plasma especially in TNBC when compared to normal individuals. Furthermore, luciferase assay identified that PIK3CD is the potential downstream target of miR-199a-5p. Conclusions: Our data implicated that miR-199a-5p confers tumor suppressive role in TNBC, which inhibited stemness characteristics. These findings may help the development of novel therapeutic strategies towards this highly malignant disease. | - |
dc.language | eng | - |
dc.publisher | Korea Breast Cancer Society. | - |
dc.relation.ispartof | Global Breast Cancer Conference (GBCC), 2016 | - |
dc.title | TUMOR SUPPRESSIVE ROLE OF MIR-199A-5P IN TRIPLE-NEGATIVE BREAST CANCER | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Chen, J: gary0526@hku.hk | - |
dc.identifier.email | Shin, VY: vyshin@hku.hk | - |
dc.identifier.email | Siu, JMT: jensiu@hku.hk | - |
dc.identifier.email | Cheuk, WYI: isacheuk@hku.hk | - |
dc.identifier.email | Kwong, A: avakwong@hku.hk | - |
dc.identifier.authority | Shin, VY=rp02000 | - |
dc.identifier.authority | Kwong, A=rp01734 | - |
dc.identifier.hkuros | 297043 | - |
dc.identifier.spage | 258 | - |
dc.identifier.epage | 258 | - |
dc.publisher.place | Korea | - |