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Article: Partnership of Arthrobacter and Pimelobacter in Aerobic Degradation of Sulfadiazine Revealed by Metagenomics Analysis and Isolation

TitlePartnership of Arthrobacter and Pimelobacter in Aerobic Degradation of Sulfadiazine Revealed by Metagenomics Analysis and Isolation
Authors
KeywordsBacteria
Biodegradation
Bioremediation
Carbon
Issue Date2018
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/esthag
Citation
Environmental Science & Technology, 2018, v. 52 n. 5, p. 2963-2972 How to Cite?
AbstractIn this study, metagenomic analyses were combined with cultivation-based techniques as a nested approach to identify functionally significant bacteria for sulfadiazine biodegradation within enrichment communities. The metagenomic investigations indicated that our previously isolated sulfadiazine degrader, Arthrobacter sp. D2, and another Pimelobacter bacterium concomitantly occurred as most abundant members in the community of an enrichment culture that performed complete sulfadiazine mineralization for over two years. Responses of the enriched populations to sole carbon source alternation further suggested the ability of this Pimelobacter member to grow on 2-aminopyrimidine, the most prominent intermediate metabolite of sulfadiazine. Taking advantage of this propensity, additional cultivation procedures have enabled the successful isolation of Pimelobacter sp. LG209, whose genomic sequences exactly matched that of the dominant Pimelobacter bacterium in the sulfadiazine enrichment culture. Integration of metagenomic investigations with the physiological characteristics of the isolates conclusively demonstrated that the sulfadiazine mineralization in a long-running enrichment culture was prominently mediated by primary sulfadiazine-degrading specialist strain Arthrobacter sp. D2 in association with the 2-aminopyrimidine-degrading partner strain Pimelobacter sp. LG209. Here, we provided the first mechanistic insight into microbial interactions in steady sulfadiazine mineralization processes, which will help develop appropriate bioremediation strategies for sulfadiazine-contaminated hotspot sites.
Persistent Identifierhttp://hdl.handle.net/10722/272151
ISSN
2019 Impact Factor: 7.864
2015 SCImago Journal Rankings: 2.664
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorDeng, Y-
dc.contributor.authorWang, Y-
dc.contributor.authorMao, Y-
dc.contributor.authorZhang, T-
dc.date.accessioned2019-07-20T10:36:40Z-
dc.date.available2019-07-20T10:36:40Z-
dc.date.issued2018-
dc.identifier.citationEnvironmental Science & Technology, 2018, v. 52 n. 5, p. 2963-2972-
dc.identifier.issn0013-936X-
dc.identifier.urihttp://hdl.handle.net/10722/272151-
dc.description.abstractIn this study, metagenomic analyses were combined with cultivation-based techniques as a nested approach to identify functionally significant bacteria for sulfadiazine biodegradation within enrichment communities. The metagenomic investigations indicated that our previously isolated sulfadiazine degrader, Arthrobacter sp. D2, and another Pimelobacter bacterium concomitantly occurred as most abundant members in the community of an enrichment culture that performed complete sulfadiazine mineralization for over two years. Responses of the enriched populations to sole carbon source alternation further suggested the ability of this Pimelobacter member to grow on 2-aminopyrimidine, the most prominent intermediate metabolite of sulfadiazine. Taking advantage of this propensity, additional cultivation procedures have enabled the successful isolation of Pimelobacter sp. LG209, whose genomic sequences exactly matched that of the dominant Pimelobacter bacterium in the sulfadiazine enrichment culture. Integration of metagenomic investigations with the physiological characteristics of the isolates conclusively demonstrated that the sulfadiazine mineralization in a long-running enrichment culture was prominently mediated by primary sulfadiazine-degrading specialist strain Arthrobacter sp. D2 in association with the 2-aminopyrimidine-degrading partner strain Pimelobacter sp. LG209. Here, we provided the first mechanistic insight into microbial interactions in steady sulfadiazine mineralization processes, which will help develop appropriate bioremediation strategies for sulfadiazine-contaminated hotspot sites.-
dc.languageeng-
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journal/esthag-
dc.relation.ispartofEnvironmental Science & Technology-
dc.subjectBacteria-
dc.subjectBiodegradation-
dc.subjectBioremediation-
dc.subjectCarbon-
dc.titlePartnership of Arthrobacter and Pimelobacter in Aerobic Degradation of Sulfadiazine Revealed by Metagenomics Analysis and Isolation-
dc.typeArticle-
dc.identifier.emailDeng, Y: dengyu@hku.hk-
dc.identifier.emailZhang, T: zhangt@hkucc.hku.hk-
dc.identifier.authorityZhang, T=rp00211-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/acs.est.7b05913-
dc.identifier.pmid29378398-
dc.identifier.scopuseid_2-s2.0-85043478505-
dc.identifier.hkuros299348-
dc.identifier.volume52-
dc.identifier.issue5-
dc.identifier.spage2963-
dc.identifier.epage2972-
dc.identifier.isiWOS:000427202700061-
dc.publisher.placeUnited States-

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