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Article: Population dynamics of a dual Pseudomonas putida–Pseudomonas fluorescens biofilm in a capillary bioreactor

TitlePopulation dynamics of a dual Pseudomonas putida–Pseudomonas fluorescens biofilm in a capillary bioreactor
Authors
KeywordsDetachment
Lipopeptide
Mixed-species biofilm
Issue Date2019
PublisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/08927014.asp
Citation
Biofouling, 2019, v. 35 n. 3, p. 299-307 How to Cite?
AbstractMost biofilm studies employ single species, yet in nature biofilms exist as mixed cultures, with inevitable effects on growth and development of each species present. To investigate how related species of bacteria interact in biofilms, two Pseudomonas spp., Pseudomonas fluorescens and Pseudomonas putida, were cultured in capillary bioreactors and their growth measured by confocal microscopy and cell counting. When inoculated in pure culture, both bacteria formed healthy biofilms within 72 h with uniform coverage of the surface. However, when the bioreactors were inoculated with both bacteria simultaneously, P. putida was completely dominant after 48 h. Even when the inoculation by P. putida was delayed for 24 h, P. fluorescens was eliminated from the capillary within 48 h. It is proposed that production of the lipopeptide putisolvin by P. putida is the likely reason for the reduction of P. fluorescens. Putisolvin biosynthesis in the dual-species biofilm was confirmed by mass spectrometry.
Persistent Identifierhttp://hdl.handle.net/10722/272497
ISSN
2019 Impact Factor: 2.351
2015 SCImago Journal Rankings: 1.219
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorGazzola, G-
dc.contributor.authorHabimana, O-
dc.contributor.authorQuinn, L-
dc.contributor.authorCasey, E-
dc.contributor.authorMurphy, CD-
dc.date.accessioned2019-07-20T10:43:26Z-
dc.date.available2019-07-20T10:43:26Z-
dc.date.issued2019-
dc.identifier.citationBiofouling, 2019, v. 35 n. 3, p. 299-307-
dc.identifier.issn0892-7014-
dc.identifier.urihttp://hdl.handle.net/10722/272497-
dc.description.abstractMost biofilm studies employ single species, yet in nature biofilms exist as mixed cultures, with inevitable effects on growth and development of each species present. To investigate how related species of bacteria interact in biofilms, two Pseudomonas spp., Pseudomonas fluorescens and Pseudomonas putida, were cultured in capillary bioreactors and their growth measured by confocal microscopy and cell counting. When inoculated in pure culture, both bacteria formed healthy biofilms within 72 h with uniform coverage of the surface. However, when the bioreactors were inoculated with both bacteria simultaneously, P. putida was completely dominant after 48 h. Even when the inoculation by P. putida was delayed for 24 h, P. fluorescens was eliminated from the capillary within 48 h. It is proposed that production of the lipopeptide putisolvin by P. putida is the likely reason for the reduction of P. fluorescens. Putisolvin biosynthesis in the dual-species biofilm was confirmed by mass spectrometry.-
dc.languageeng-
dc.publisherTaylor & Francis Ltd. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/08927014.asp-
dc.relation.ispartofBiofouling-
dc.subjectDetachment-
dc.subjectLipopeptide-
dc.subjectMixed-species biofilm-
dc.titlePopulation dynamics of a dual Pseudomonas putida–Pseudomonas fluorescens biofilm in a capillary bioreactor-
dc.typeArticle-
dc.identifier.emailHabimana, O: ohabim@hku.hk-
dc.identifier.authorityHabimana, O=rp02169-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1080/08927014.2019.1598397-
dc.identifier.pmid31025575-
dc.identifier.scopuseid_2-s2.0-85065072206-
dc.identifier.hkuros299239-
dc.identifier.volume35-
dc.identifier.issue3-
dc.identifier.spage299-
dc.identifier.epage307-
dc.identifier.isiWOS:000470614100001-
dc.publisher.placeUnited Kingdom-

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