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- Publisher Website: 10.3389/fchem.2019.00560
- Scopus: eid_2-s2.0-85072739369
- PMID: 31448265
- WOS: WOS:000480247000001
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Article: Recognition of proteins by metal-chelation based fluorescent probes in cells
| Title | Recognition of proteins by metal-chelation based fluorescent probes in cells |
|---|---|
| Authors | |
| Keywords | His-tag metallomics metalloproteomics molecular imaging thiol-reaction |
| Issue Date | 2019 |
| Publisher | Frontiers Media SA. The Journal's web site is located at http://www.frontiersin.org/chemistry |
| Citation | Frontiers in Chemistry, 2019 How to Cite? |
| Abstract | Fluorescent probes such as thiol-reactive and Ni2+-nitrilotriacetate (NTA) based probes provide a powerful toolbox for real-time visualization of a protein and a proteome in living cells. Herein, we first went through basic principles and applications of thiol-reactive based probes in protein imaging and recognition. We then summarize a family of metal-NTA based fluorescence probes in the visualization of His(6)-tagged protein and identification of metalloproteins at proteome-wide scale. The pros and cons of the probes, as well as ways to optimize them, are discussed. |
| Persistent Identifier | http://hdl.handle.net/10722/273841 |
| ISSN | 2021 Impact Factor: 5.545 2020 SCImago Journal Rankings: 1.027 |
| ISI Accession Number ID |
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Jiang, N | - |
| dc.contributor.author | Li, H | - |
| dc.contributor.author | Sun, H | - |
| dc.date.accessioned | 2019-08-18T14:49:39Z | - |
| dc.date.available | 2019-08-18T14:49:39Z | - |
| dc.date.issued | 2019 | - |
| dc.identifier.citation | Frontiers in Chemistry, 2019 | - |
| dc.identifier.issn | 2296-2646 | - |
| dc.identifier.uri | http://hdl.handle.net/10722/273841 | - |
| dc.description.abstract | Fluorescent probes such as thiol-reactive and Ni2+-nitrilotriacetate (NTA) based probes provide a powerful toolbox for real-time visualization of a protein and a proteome in living cells. Herein, we first went through basic principles and applications of thiol-reactive based probes in protein imaging and recognition. We then summarize a family of metal-NTA based fluorescence probes in the visualization of His(6)-tagged protein and identification of metalloproteins at proteome-wide scale. The pros and cons of the probes, as well as ways to optimize them, are discussed. | - |
| dc.language | eng | - |
| dc.publisher | Frontiers Media SA. The Journal's web site is located at http://www.frontiersin.org/chemistry | - |
| dc.relation.ispartof | Frontiers in Chemistry | - |
| dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
| dc.subject | His-tag | - |
| dc.subject | metallomics | - |
| dc.subject | metalloproteomics | - |
| dc.subject | molecular imaging | - |
| dc.subject | thiol-reaction | - |
| dc.title | Recognition of proteins by metal-chelation based fluorescent probes in cells | - |
| dc.type | Article | - |
| dc.identifier.email | Li, H: hylichem@hku.hk | - |
| dc.identifier.email | Sun, H: hsun@hku.hk | - |
| dc.identifier.authority | Sun, H=rp00777 | - |
| dc.description.nature | published_or_final_version | - |
| dc.identifier.doi | 10.3389/fchem.2019.00560 | - |
| dc.identifier.pmid | 31448265 | - |
| dc.identifier.scopus | eid_2-s2.0-85072739369 | - |
| dc.identifier.hkuros | 301440 | - |
| dc.identifier.isi | WOS:000480247000001 | - |
| dc.publisher.place | Switzerland | - |
| dc.identifier.issnl | 2296-2646 | - |