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- Publisher Website: 10.1364/OL.44.000391
- Scopus: eid_2-s2.0-85059988659
- PMID: 30644908
- WOS: WOS:000455620100051
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Article: Volumetric two-photon microscopy with a non-diffracting Airy beam
Title | Volumetric two-photon microscopy with a non-diffracting Airy beam |
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Authors | |
Keywords | Computerized tomography Photons Image Structures Penetration ability Propagation direction |
Issue Date | 2019 |
Publisher | Optical Society of America. The Journal's web site is located at http://www.opticsinfobase.org/current.cfm?journal=3 |
Citation | Optics Letters, 2019, v. 44 n. 2, p. 391-394 How to Cite? |
Abstract | We demonstrate a volumetric two-photon microscopy (TPM) using the non-diffracting Airy beam as illumination. Direct mapping of the imaging trajectory shows that the Airy beam extends the axial imaging range around six times longer than a traditional Gaussian beam does along the propagation direction, while maintaining a comparable lateral width. Benefiting from its non-diffracting nature, the TPM with Airy beam illumination is able not only to capture a volumetric image within a single frame, but also to acquire image structures behind a strongly scattered medium. The volumetric specimen is mapped layer by layer under Gaussian mode, while the three-dimensional structure is projected to a single two-dimensional image under Airy mode, leading to a significantly increased acquisition speed. The performance of the TPM is evaluated employing a phantom of agarose gel imbedding fluorescent beads as well as a mouse brain slice. Finally, we showcase the penetration ability of the developed Airy TPM by imaging through a scattering environment. |
Persistent Identifier | http://hdl.handle.net/10722/274586 |
ISSN | 2023 Impact Factor: 3.1 2023 SCImago Journal Rankings: 1.040 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Tan, XJ | - |
dc.contributor.author | KONG, C | - |
dc.contributor.author | Ren, YX | - |
dc.contributor.author | Lai, SWC | - |
dc.contributor.author | Tsia, KK | - |
dc.contributor.author | Wong, KKY | - |
dc.date.accessioned | 2019-08-18T15:04:45Z | - |
dc.date.available | 2019-08-18T15:04:45Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Optics Letters, 2019, v. 44 n. 2, p. 391-394 | - |
dc.identifier.issn | 0146-9592 | - |
dc.identifier.uri | http://hdl.handle.net/10722/274586 | - |
dc.description.abstract | We demonstrate a volumetric two-photon microscopy (TPM) using the non-diffracting Airy beam as illumination. Direct mapping of the imaging trajectory shows that the Airy beam extends the axial imaging range around six times longer than a traditional Gaussian beam does along the propagation direction, while maintaining a comparable lateral width. Benefiting from its non-diffracting nature, the TPM with Airy beam illumination is able not only to capture a volumetric image within a single frame, but also to acquire image structures behind a strongly scattered medium. The volumetric specimen is mapped layer by layer under Gaussian mode, while the three-dimensional structure is projected to a single two-dimensional image under Airy mode, leading to a significantly increased acquisition speed. The performance of the TPM is evaluated employing a phantom of agarose gel imbedding fluorescent beads as well as a mouse brain slice. Finally, we showcase the penetration ability of the developed Airy TPM by imaging through a scattering environment. | - |
dc.language | eng | - |
dc.publisher | Optical Society of America. The Journal's web site is located at http://www.opticsinfobase.org/current.cfm?journal=3 | - |
dc.relation.ispartof | Optics Letters | - |
dc.rights | Optics Letters. Copyright © Optical Society of America. | - |
dc.rights | © XXXX [year] Optical Society of America]. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modifications of the content of this paper are prohibited. | - |
dc.subject | Computerized tomography | - |
dc.subject | Photons | - |
dc.subject | Image Structures | - |
dc.subject | Penetration ability | - |
dc.subject | Propagation direction | - |
dc.title | Volumetric two-photon microscopy with a non-diffracting Airy beam | - |
dc.type | Article | - |
dc.identifier.email | Tan, XJ: xjtan@HKUCC-COM.hku.hk | - |
dc.identifier.email | Ren, YX: yxren@hku.hk | - |
dc.identifier.email | Lai, SWC: coraswl@hku.hk | - |
dc.identifier.email | Tsia, KK: tsia@hku.hk | - |
dc.identifier.email | Wong, KKY: kywong@eee.hku.hk | - |
dc.identifier.authority | Lai, SWC=rp01895 | - |
dc.identifier.authority | Tsia, KK=rp01389 | - |
dc.identifier.authority | Wong, KKY=rp00189 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1364/OL.44.000391 | - |
dc.identifier.pmid | 30644908 | - |
dc.identifier.scopus | eid_2-s2.0-85059988659 | - |
dc.identifier.hkuros | 302161 | - |
dc.identifier.hkuros | 319013 | - |
dc.identifier.volume | 44 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 391 | - |
dc.identifier.epage | 394 | - |
dc.identifier.isi | WOS:000455620100051 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0146-9592 | - |