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Conference Paper: Abnormal retinal function after oxygen-induced retinopathy under maternal hyperglycemic condition
Title | Abnormal retinal function after oxygen-induced retinopathy under maternal hyperglycemic condition |
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Authors | |
Issue Date | 2018 |
Publisher | International Conference of Vision & Eye Research. |
Citation | The International Conference of Vision and Eye Research (iCover), Hong Kong, 8-10 November 2018 How to Cite? |
Abstract | Purpose: Maternal diabetes is associated with retinopathy of prematurity (ROP). The influence of maternal diabetes on ROP progression and severity is investigated in retina of pups from hyperglycemic C57Bl/6 Ins2Akita/J (Akita) mothers subjected to oxygen-induced retinopathy (OIR).
Methods: Genotyping and blood glucose measurement of Akita mice were performed before 10 weeks of age. The pups and their nursing mother were placed into a chamber with 75% oxygen from postnatal day (P)7 to P12 and subsequently returned to room air. Pups from C57Bl/6J (wildtype, WT) or Akita mothers were sacrificed on P7, 12, 17, 21, and 30. The retinal vascular network was visualized using isolectin B4 immunostaining and retinal function was assessed by electroretinography (ERG).
Results: Hyperglycemia was well-developed in 10-week-old Akita mothers. They displayed higher pregnancy risks including dystocia and higher mortality rate of pups. The pups from Akita mothers were heavier after 21-day-old but had larger weight loss after OIR. Under room air condition, the pups from either WT or Akita mothers displayed normal retinal vascular and functional development. After OIR, despite a similar extent of avascularization, pups from Akita mothers had smaller neovascular area on P21. The a-wave and b-wave amplitudes were reduced significantly after OIR in both groups, but the pups from Akita mothers had a slower recovery rate in a-wave amplitude.
Conclusions: Normal retinal vascular and functional development were not affected by maternal hyperglycemia. However, the retinal function of neonates from hyperglycemic mothers was disrupted by OIR and showed a slower recovery rate, suggesting a sustained deficit in photoreceptor functions. |
Description | Poster Presentation Organized by the School of Optometry, The Hong Kong Polytechnic University |
Persistent Identifier | http://hdl.handle.net/10722/276199 |
DC Field | Value | Language |
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dc.contributor.author | Tsang, KW | - |
dc.contributor.author | Lo, ACY | - |
dc.date.accessioned | 2019-09-10T02:57:59Z | - |
dc.date.available | 2019-09-10T02:57:59Z | - |
dc.date.issued | 2018 | - |
dc.identifier.citation | The International Conference of Vision and Eye Research (iCover), Hong Kong, 8-10 November 2018 | - |
dc.identifier.uri | http://hdl.handle.net/10722/276199 | - |
dc.description | Poster Presentation | - |
dc.description | Organized by the School of Optometry, The Hong Kong Polytechnic University | - |
dc.description.abstract | Purpose: Maternal diabetes is associated with retinopathy of prematurity (ROP). The influence of maternal diabetes on ROP progression and severity is investigated in retina of pups from hyperglycemic C57Bl/6 Ins2Akita/J (Akita) mothers subjected to oxygen-induced retinopathy (OIR). Methods: Genotyping and blood glucose measurement of Akita mice were performed before 10 weeks of age. The pups and their nursing mother were placed into a chamber with 75% oxygen from postnatal day (P)7 to P12 and subsequently returned to room air. Pups from C57Bl/6J (wildtype, WT) or Akita mothers were sacrificed on P7, 12, 17, 21, and 30. The retinal vascular network was visualized using isolectin B4 immunostaining and retinal function was assessed by electroretinography (ERG). Results: Hyperglycemia was well-developed in 10-week-old Akita mothers. They displayed higher pregnancy risks including dystocia and higher mortality rate of pups. The pups from Akita mothers were heavier after 21-day-old but had larger weight loss after OIR. Under room air condition, the pups from either WT or Akita mothers displayed normal retinal vascular and functional development. After OIR, despite a similar extent of avascularization, pups from Akita mothers had smaller neovascular area on P21. The a-wave and b-wave amplitudes were reduced significantly after OIR in both groups, but the pups from Akita mothers had a slower recovery rate in a-wave amplitude. Conclusions: Normal retinal vascular and functional development were not affected by maternal hyperglycemia. However, the retinal function of neonates from hyperglycemic mothers was disrupted by OIR and showed a slower recovery rate, suggesting a sustained deficit in photoreceptor functions. | - |
dc.language | eng | - |
dc.publisher | International Conference of Vision & Eye Research. | - |
dc.relation.ispartof | International Conference of Vision & Eye Research 2018 (iCOVER 2018) | - |
dc.title | Abnormal retinal function after oxygen-induced retinopathy under maternal hyperglycemic condition | - |
dc.type | Conference_Paper | - |
dc.identifier.email | Lo, ACY: amylo@hku.hk | - |
dc.identifier.authority | Lo, ACY=rp00425 | - |
dc.identifier.hkuros | 303214 | - |