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Article: A new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating

TitleA new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating
Authors
Issue Date2018
PublisherNature Publishing Group: Open Access Journals - Option C. The Journal's web site is located at http://www.nature.com/celldisc/
Citation
Cell Discovery, 2018, v. 4, article no. 65 (2018) How to Cite?
AbstractKif5b-driven anterograde transport and clathrin-mediated endocytosis (CME) are responsible for opposite intracellular trafficking, contributing to plasma membrane homeostasis. However, whether and how the two trafficking processes coordinate remain unclear. Here, we show that Kif5b directly interacts with clathrin heavy chain (CHC) at a region close to that for uncoating catalyst (Hsc70) and preferentially localizes on relatively large clathrin-coated vesicles (CCVs). Uncoating in vitro is decreased for CCVs from the cortex of kif5b conditional knockout (mutant) mouse and facilitated by adding Kif5b fragments containing CHC-binding site, while cell peripheral distribution of CHC or Hsc70 keeps unaffected by Kif5b depletion. Furthermore, cellular entry of vesicular stomatitis virus that internalizes into large CCV is inhibited by Kif5b depletion or introducing a dominant-negative Kif5b fragment. These findings showed a new role of Kif5b in regulating large CCV-mediated CME via affecting CCV uncoating, indicating Kif5b as a molecular knot connecting anterograde transport to CME.
Persistent Identifierhttp://hdl.handle.net/10722/276216
ISSN
2017 Impact Factor: 4.462
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorZhou, N-
dc.contributor.authorYung, WH-
dc.contributor.authorLin, RZ-
dc.contributor.authorDuan, ZG-
dc.contributor.authorLiu, MF-
dc.contributor.authorNi, YX-
dc.contributor.authorNi, YX-
dc.contributor.authorXue, WQ-
dc.contributor.authorRong, Li-
dc.contributor.authorKao, RYT-
dc.contributor.authorSun, HT-
dc.contributor.authorGong, HR-
dc.contributor.authorTang, XM-
dc.contributor.authorZhang, W-
dc.contributor.authorChung, SK-
dc.contributor.authorSong, Y-
dc.contributor.authorHuang, JD-
dc.date.accessioned2019-09-10T02:58:20Z-
dc.date.available2019-09-10T02:58:20Z-
dc.date.issued2018-
dc.identifier.citationCell Discovery, 2018, v. 4, article no. 65 (2018)-
dc.identifier.issn2056-5968-
dc.identifier.urihttp://hdl.handle.net/10722/276216-
dc.description.abstractKif5b-driven anterograde transport and clathrin-mediated endocytosis (CME) are responsible for opposite intracellular trafficking, contributing to plasma membrane homeostasis. However, whether and how the two trafficking processes coordinate remain unclear. Here, we show that Kif5b directly interacts with clathrin heavy chain (CHC) at a region close to that for uncoating catalyst (Hsc70) and preferentially localizes on relatively large clathrin-coated vesicles (CCVs). Uncoating in vitro is decreased for CCVs from the cortex of kif5b conditional knockout (mutant) mouse and facilitated by adding Kif5b fragments containing CHC-binding site, while cell peripheral distribution of CHC or Hsc70 keeps unaffected by Kif5b depletion. Furthermore, cellular entry of vesicular stomatitis virus that internalizes into large CCV is inhibited by Kif5b depletion or introducing a dominant-negative Kif5b fragment. These findings showed a new role of Kif5b in regulating large CCV-mediated CME via affecting CCV uncoating, indicating Kif5b as a molecular knot connecting anterograde transport to CME.-
dc.languageeng-
dc.publisherNature Publishing Group: Open Access Journals - Option C. The Journal's web site is located at http://www.nature.com/celldisc/-
dc.relation.ispartofCell Discovery-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleA new role of anterograde motor Kif5b in facilitating large clathrin-coated vesicle mediated endocytosis via regulating clathrin uncoating-
dc.typeArticle-
dc.identifier.emailKao, RYT: rytkao@hkucc.hku.hk-
dc.identifier.emailChung, SK: skchung@hkucc.hku.hk-
dc.identifier.emailSong, Y: songy@hku.hk-
dc.identifier.authorityKao, RYT=rp00481-
dc.identifier.authorityChung, SK=rp00381-
dc.identifier.authoritySong, Y=rp00488-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1038/s41421-018-0067-5-
dc.identifier.scopuseid_2-s2.0-85058982266-
dc.identifier.hkuros302617-
dc.identifier.volume4-
dc.identifier.spagearticle no. 65 (2018)-
dc.identifier.epagearticle no. 65 (2018)-
dc.identifier.isiWOS:000454258200001-
dc.publisher.placeUnited Kingdom-

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