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Article: Cis‐control of Six1 expression in neural crest cells during craniofacial development

TitleCis‐control of Six1 expression in neural crest cells during craniofacial development
Authors
Keywordscraniofacial development
enhancer
neural crest
Six1
Issue Date2019
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/38417
Citation
Developmental Dynamics, 2019, v. 248 n. 12, p. 1264-1272 How to Cite?
AbstractBackground Six1 is a transcriptional factor that plays an important role in embryonic development. Mouse and chick embryos deficient for Six1 have multiple craniofacial anomalies in the facial bones and cartilages. Multiple Six1 enhancers have been identified, but none of them has been reported to be active in the maxillary and mandibular process. Results We studied two Six1 enhancers in the chick neural crest tissues during craniofacial development. We showed that two evolutionarily conserved enhancers, Six1E1 and Six1E2, act synergistically. Neither Six1E1 nor Six1E2 alone can drive enhancer reporter signal in the maxillary or mandibular processes. However, their combination, Six1E, showed robust enhancer activity in these tissues. Similar reporter signal can also be driven by the mouse homolog of Six1E. Mutations of multiple conserved transcriptional factor binding sites altered the enhancer activity of Six1E, especially mutation of the LIM homeobox binding site, dramatically reduced the enhancer activity, implying that the Lhx protein family be an important regulator of Six1 expression. Conclusion This study, for the first time, described the synergistic activation of two Six1 enhancers in the maxillary and mandibular processes, and will facilitate more detailed studies of the regulation of Six1 in craniofacial development.
Persistent Identifierhttp://hdl.handle.net/10722/276241
ISSN
2017 Impact Factor: 2.507
2015 SCImago Journal Rankings: 1.726
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorWu, Z-
dc.contributor.authorRao, Y-
dc.contributor.authorZhang, S-
dc.contributor.authorKim, EJ-
dc.contributor.authorOki, S-
dc.contributor.authorHarada, H-
dc.contributor.authorCheung, M-
dc.contributor.authorJung, HS-
dc.date.accessioned2019-09-10T02:58:52Z-
dc.date.available2019-09-10T02:58:52Z-
dc.date.issued2019-
dc.identifier.citationDevelopmental Dynamics, 2019, v. 248 n. 12, p. 1264-1272-
dc.identifier.issn1058-8388-
dc.identifier.urihttp://hdl.handle.net/10722/276241-
dc.description.abstractBackground Six1 is a transcriptional factor that plays an important role in embryonic development. Mouse and chick embryos deficient for Six1 have multiple craniofacial anomalies in the facial bones and cartilages. Multiple Six1 enhancers have been identified, but none of them has been reported to be active in the maxillary and mandibular process. Results We studied two Six1 enhancers in the chick neural crest tissues during craniofacial development. We showed that two evolutionarily conserved enhancers, Six1E1 and Six1E2, act synergistically. Neither Six1E1 nor Six1E2 alone can drive enhancer reporter signal in the maxillary or mandibular processes. However, their combination, Six1E, showed robust enhancer activity in these tissues. Similar reporter signal can also be driven by the mouse homolog of Six1E. Mutations of multiple conserved transcriptional factor binding sites altered the enhancer activity of Six1E, especially mutation of the LIM homeobox binding site, dramatically reduced the enhancer activity, implying that the Lhx protein family be an important regulator of Six1 expression. Conclusion This study, for the first time, described the synergistic activation of two Six1 enhancers in the maxillary and mandibular processes, and will facilitate more detailed studies of the regulation of Six1 in craniofacial development.-
dc.languageeng-
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/38417-
dc.relation.ispartofDevelopmental Dynamics-
dc.rightsPreprint This is the pre-peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. Postprint This is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.-
dc.subjectcraniofacial development-
dc.subjectenhancer-
dc.subjectneural crest-
dc.subjectSix1-
dc.titleCis‐control of Six1 expression in neural crest cells during craniofacial development-
dc.typeArticle-
dc.identifier.emailCheung, M: mcheung9@hku.hk-
dc.identifier.authorityCheung, M=rp00245-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/dvdy.109-
dc.identifier.pmid31464047-
dc.identifier.scopuseid_2-s2.0-85073832165-
dc.identifier.hkuros304968-
dc.identifier.volume248-
dc.identifier.issue12-
dc.identifier.spage1264-
dc.identifier.epage1272-
dc.identifier.isiWOS:000486686000001-
dc.publisher.placeUnited States-

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