File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: A Microfluidic System for One‐Chip Harvesting of Single‐Cell‐Laden Hydrogels in Culture Medium

TitleA Microfluidic System for One‐Chip Harvesting of Single‐Cell‐Laden Hydrogels in Culture Medium
Authors
Keywordsdroplet microfluidics
GelMA hydrogel
single cell
Issue Date2019
PublisherWiley - VCH Verlag GmbH & Co. KGaA. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2366-7478
Citation
Advanced Biosystems, 2019, v. 3 n. 11, article no. 1900076 How to Cite?
AbstractSingle‐cell analysis has shown great potential to fully quantify the distribution of cellular behaviors among a population of individuals. Through isolation and preservation of single cells in the aqueous phase, droplet encapsulation followed by gelation enables high‐throughput analysis in biocompatible microgels. However, the lack of control over the number of cells encapsulated and complicated gelation processes significantly limit its efficiency. Here, a microfluidic system for one‐chip harvesting of single‐cell‐laden microgels is presented. Through ultraviolet irradiation, an on‐chip gelation technique is seamlessly combined with droplet generation to realize high‐throughput fabrication of microscale hydrogels in microfluidic channel. Moreover, a sorting module is introduced to simultaneously complete cell‐laden microgel selection and transfer into culture medium. To demonstrate the efficiency of this method, two types of single cells are respectively encapsulated and collected, showing desirable single‐cell encapsulation and cell viability. This technique realizes integrated droplet gelation, microgel sorting, and transfer into culture medium, allowing high‐throughput analysis of single cells and comprehensive understanding of the cellular specificity.
Persistent Identifierhttp://hdl.handle.net/10722/277113
ISSN
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorNan, L-
dc.contributor.authorYang, Z-
dc.contributor.authorLyu, H-
dc.contributor.authorLau, KYY-
dc.contributor.authorShum, HC-
dc.date.accessioned2019-09-20T08:44:40Z-
dc.date.available2019-09-20T08:44:40Z-
dc.date.issued2019-
dc.identifier.citationAdvanced Biosystems, 2019, v. 3 n. 11, article no. 1900076-
dc.identifier.issn2366-7478-
dc.identifier.urihttp://hdl.handle.net/10722/277113-
dc.description.abstractSingle‐cell analysis has shown great potential to fully quantify the distribution of cellular behaviors among a population of individuals. Through isolation and preservation of single cells in the aqueous phase, droplet encapsulation followed by gelation enables high‐throughput analysis in biocompatible microgels. However, the lack of control over the number of cells encapsulated and complicated gelation processes significantly limit its efficiency. Here, a microfluidic system for one‐chip harvesting of single‐cell‐laden microgels is presented. Through ultraviolet irradiation, an on‐chip gelation technique is seamlessly combined with droplet generation to realize high‐throughput fabrication of microscale hydrogels in microfluidic channel. Moreover, a sorting module is introduced to simultaneously complete cell‐laden microgel selection and transfer into culture medium. To demonstrate the efficiency of this method, two types of single cells are respectively encapsulated and collected, showing desirable single‐cell encapsulation and cell viability. This technique realizes integrated droplet gelation, microgel sorting, and transfer into culture medium, allowing high‐throughput analysis of single cells and comprehensive understanding of the cellular specificity.-
dc.languageeng-
dc.publisherWiley - VCH Verlag GmbH & Co. KGaA. The Journal's web site is located at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)2366-7478-
dc.relation.ispartofAdvanced Biosystems-
dc.rightsThis is the peer reviewed version of the following article: [FULL CITE], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.-
dc.subjectdroplet microfluidics-
dc.subjectGelMA hydrogel-
dc.subjectsingle cell-
dc.titleA Microfluidic System for One‐Chip Harvesting of Single‐Cell‐Laden Hydrogels in Culture Medium-
dc.typeArticle-
dc.identifier.emailLyu, H: lvhao@HKUCC-COM.hku.hk-
dc.identifier.emailShum, HC: ashum@hku.hk-
dc.identifier.authorityShum, HC=rp01439-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/adbi.201900076-
dc.identifier.scopuseid_2-s2.0-85067004390-
dc.identifier.hkuros305919-
dc.identifier.volume3-
dc.identifier.issue11-
dc.identifier.spagearticle no. 1900076-
dc.identifier.epagearticle no. 1900076-
dc.identifier.isiWOS:000498192300008-
dc.publisher.placeGermany-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats