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- Publisher Website: 10.1083/jcb.201801081
- Scopus: eid_2-s2.0-85069266517
- PMID: 31171630
- WOS: WOS:000473328200026
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Article: ISM1 regulates NODAL signaling and asymmetric organ morphogenesis during development
Title | ISM1 regulates NODAL signaling and asymmetric organ morphogenesis during development |
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Authors | |
Keywords | Xenopus Embryonic Structures Cell fate |
Issue Date | 2019 |
Publisher | Rockefeller University Press. The Journal's web site is located at http://www.jcb.org |
Citation | The Journal of Cell Biology, 2019, v. 218 n. 7, p. 2388-2402 How to Cite? |
Abstract | Isthmin1 (ISM1) was originally identified as a fibroblast group factor expressed in Xenopus laevis embryonic brain, but its biological functions remain unclear. The spatiotemporal distribution of ISM1, with high expression in the anterior primitive streak of the chick embryo and the anterior mesendoderm of the mouse embryo, suggested that ISM1 may regulate signaling by the NODAL subfamily of TGB-β cytokines that control embryo patterning. We report that ISM1 is an inhibitor of NODAL signaling. ISM1 has little effect on TGF-β1, ACTIVIN-A, or BMP4 signaling but specifically inhibits NODAL-induced phosphorylation of SMAD2. In line with this observation, ectopic ISM1 causes defective left-right asymmetry and abnormal heart positioning in chick embryos. Mechanistically, ISM1 interacts with NODAL ligand and type I receptor ACVR1B through its AMOP domain, which compromises the NODAL–ACVR1B interaction and down-regulates phosphorylation of SMAD2. Therefore, we identify ISM1 as an extracellular antagonist of NODAL and reveal a negative regulatory mechanism that provides greater plasticity for the fine-tuning of NODAL signaling. |
Persistent Identifier | http://hdl.handle.net/10722/277432 |
ISSN | 2023 Impact Factor: 7.4 2023 SCImago Journal Rankings: 3.717 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Osorio, L | - |
dc.contributor.author | Wu, X | - |
dc.contributor.author | WANG, L | - |
dc.contributor.author | JIANG, Z | - |
dc.contributor.author | Neideck, C | - |
dc.contributor.author | Sheng, G | - |
dc.contributor.author | Zhou, Z | - |
dc.date.accessioned | 2019-09-20T08:50:57Z | - |
dc.date.available | 2019-09-20T08:50:57Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | The Journal of Cell Biology, 2019, v. 218 n. 7, p. 2388-2402 | - |
dc.identifier.issn | 0021-9525 | - |
dc.identifier.uri | http://hdl.handle.net/10722/277432 | - |
dc.description.abstract | Isthmin1 (ISM1) was originally identified as a fibroblast group factor expressed in Xenopus laevis embryonic brain, but its biological functions remain unclear. The spatiotemporal distribution of ISM1, with high expression in the anterior primitive streak of the chick embryo and the anterior mesendoderm of the mouse embryo, suggested that ISM1 may regulate signaling by the NODAL subfamily of TGB-β cytokines that control embryo patterning. We report that ISM1 is an inhibitor of NODAL signaling. ISM1 has little effect on TGF-β1, ACTIVIN-A, or BMP4 signaling but specifically inhibits NODAL-induced phosphorylation of SMAD2. In line with this observation, ectopic ISM1 causes defective left-right asymmetry and abnormal heart positioning in chick embryos. Mechanistically, ISM1 interacts with NODAL ligand and type I receptor ACVR1B through its AMOP domain, which compromises the NODAL–ACVR1B interaction and down-regulates phosphorylation of SMAD2. Therefore, we identify ISM1 as an extracellular antagonist of NODAL and reveal a negative regulatory mechanism that provides greater plasticity for the fine-tuning of NODAL signaling. | - |
dc.language | eng | - |
dc.publisher | Rockefeller University Press. The Journal's web site is located at http://www.jcb.org | - |
dc.relation.ispartof | The Journal of Cell Biology | - |
dc.rights | The Journal of Cell Biology. Copyright © Rockefeller University Press. | - |
dc.rights | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. | - |
dc.subject | Xenopus | - |
dc.subject | Embryonic Structures | - |
dc.subject | Cell fate | - |
dc.title | ISM1 regulates NODAL signaling and asymmetric organ morphogenesis during development | - |
dc.type | Article | - |
dc.identifier.email | Zhou, Z: zhongjun@hku.hk | - |
dc.identifier.authority | Zhou, Z=rp00503 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.1083/jcb.201801081 | - |
dc.identifier.pmid | 31171630 | - |
dc.identifier.pmcid | PMC6605798 | - |
dc.identifier.scopus | eid_2-s2.0-85069266517 | - |
dc.identifier.hkuros | 305493 | - |
dc.identifier.volume | 218 | - |
dc.identifier.issue | 7 | - |
dc.identifier.spage | 2388 | - |
dc.identifier.epage | 2402 | - |
dc.identifier.isi | WOS:000473328200026 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0021-9525 | - |