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Article: A gene delivery system containing nuclear localization signal: Increased nucleus import and transfection efficiency with the assistance of RanGAP1

TitleA gene delivery system containing nuclear localization signal: Increased nucleus import and transfection efficiency with the assistance of RanGAP1
Authors
KeywordsDegradable gene delivery system
Nucleus import
Nucleus location signal
RanGAP1
Transfection
Issue Date2017
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/actabiomat
Citation
Acta Biomaterialia, 2017, v. 48, p. 215-226 How to Cite?
AbstractIn the present report, a degradable gene delivery system (PAMS/DNA/10NLS) containing nucleus location signal peptide (NLS) was prepared. The agarose gel electrophoresis, particle size and zeta potential of PAMS/DNA/10NLS were similar to those of PAMS/DNA, which proved that NLS did not affect the interaction between PAMS and DNA. PAMS/DNA/10NLS exhibited marked extracellular and intracellular degradation under acidic conditions. The degradation was believed to allow NLS to come into contact with importins easily, which was able to mediate the nucleus import. With the help of NLS, PAMS/DNA/10NLS exhibited a higher transfection capability than PAMS/DNA. Moreover, the transfection of PAMS/DNA/10NLS was less dependent on the breakdown of the nucleus envelope than PAMS/DNA. Considering that GTPase-activating protein 1 (RanGAP1) was able to activate the endogenous GTPase, which was necessary for NLS-mediated nucleus import, RanGAP1 overexpressed cells (RanGAP1 cells) were produced. This result showed that RanGAP1 cells had higher GTPase activities than normal cells. Both the nucleus import and transfection efficiency of PAMS/DNA/10NLS were markedly higher in RanGAP1 cells than that in normal cells. The in vivo transfection results also showed that the transfection efficiency of PAMS/DNA/10NLS was higher in RanGAP1 pre-treated mice than that in normal mice. These findings showed that PAMS/DNA/10NLS is a promising gene delivery system with the assistance of RanGAP1.
Persistent Identifierhttp://hdl.handle.net/10722/277720
ISSN
2017 Impact Factor: 6.383
2015 SCImago Journal Rankings: 2.020
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChen, K-
dc.contributor.authorGuo, L-
dc.contributor.authorZhang, J-
dc.contributor.authorChen, Q-
dc.contributor.authorWang, K-
dc.contributor.authorLi, C-
dc.contributor.authorLi, W-
dc.contributor.authorQiao, M-
dc.contributor.authorZhao, X-
dc.contributor.authorHu, H-
dc.contributor.authorChen, D-
dc.date.accessioned2019-10-03T02:26:01Z-
dc.date.available2019-10-03T02:26:01Z-
dc.date.issued2017-
dc.identifier.citationActa Biomaterialia, 2017, v. 48, p. 215-226-
dc.identifier.issn1742-7061-
dc.identifier.urihttp://hdl.handle.net/10722/277720-
dc.description.abstractIn the present report, a degradable gene delivery system (PAMS/DNA/10NLS) containing nucleus location signal peptide (NLS) was prepared. The agarose gel electrophoresis, particle size and zeta potential of PAMS/DNA/10NLS were similar to those of PAMS/DNA, which proved that NLS did not affect the interaction between PAMS and DNA. PAMS/DNA/10NLS exhibited marked extracellular and intracellular degradation under acidic conditions. The degradation was believed to allow NLS to come into contact with importins easily, which was able to mediate the nucleus import. With the help of NLS, PAMS/DNA/10NLS exhibited a higher transfection capability than PAMS/DNA. Moreover, the transfection of PAMS/DNA/10NLS was less dependent on the breakdown of the nucleus envelope than PAMS/DNA. Considering that GTPase-activating protein 1 (RanGAP1) was able to activate the endogenous GTPase, which was necessary for NLS-mediated nucleus import, RanGAP1 overexpressed cells (RanGAP1 cells) were produced. This result showed that RanGAP1 cells had higher GTPase activities than normal cells. Both the nucleus import and transfection efficiency of PAMS/DNA/10NLS were markedly higher in RanGAP1 cells than that in normal cells. The in vivo transfection results also showed that the transfection efficiency of PAMS/DNA/10NLS was higher in RanGAP1 pre-treated mice than that in normal mice. These findings showed that PAMS/DNA/10NLS is a promising gene delivery system with the assistance of RanGAP1.-
dc.languageeng-
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/actabiomat-
dc.relation.ispartofActa Biomaterialia-
dc.subjectDegradable gene delivery system-
dc.subjectNucleus import-
dc.subjectNucleus location signal-
dc.subjectRanGAP1-
dc.subjectTransfection-
dc.titleA gene delivery system containing nuclear localization signal: Increased nucleus import and transfection efficiency with the assistance of RanGAP1-
dc.typeArticle-
dc.identifier.emailChen, K: kcjackie@hku.hk-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.actbio.2016.11.004-
dc.identifier.pmid27816620-
dc.identifier.scopuseid_2-s2.0-85006454832-
dc.identifier.hkuros304447-
dc.identifier.volume48-
dc.identifier.spage215-
dc.identifier.epage226-
dc.identifier.isiWOS:000393247000018-
dc.publisher.placeNetherlands-

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