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Conference Paper: Enhanced Osteogenic Differentiation by cAMP-loaded Layer-by-layer Self-assembly on Gelatin-Based Hydroxyapatite

TitleEnhanced Osteogenic Differentiation by cAMP-loaded Layer-by-layer Self-assembly on Gelatin-Based Hydroxyapatite
Authors
Issue Date2019
PublisherInternational Association for Dental Research. The Journal's web site is located at http://www.iadr.org/
Citation
The 97th General Session of the International Association of Dental Research (IADR) held with the 48th Annual Meeting of the American Association for Dental Research (AADR) & the 43rd Annual Meeting of the Canadian Association for Dental Research (CADR), Vancouver, BC, Canada, 19-22 June 2019. In Journal of Dental Research, 2019, v. 98 n. Spec ISS A, article no. 1553 How to Cite?
AbstractObjectives: To investigate osteogenic differentiation of cyclic adenosine monophosphate (cAMP) encapsulated stem cells from apical papilla (SCAPs) by layer-by-layer (LBL) self-assembly on gelatin based hydroxyapatite (HA) scaffold. Methods: Gelatin gel were alternately soaked in solution of CaCl2 and Na2HPO4 to prepare the Gel-HA scaffold. The physico-chemical characteristics of Gel-HA were analyzed by scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX). SCAPs were encapsulated with cAMP using LBL self-assembled gelatin and alginate polyelectrolytes (LBL-cAMP-SCAPs). SCAPs loaded LBL self-assembled without cAMP was used as negative control (LBL-SCAPs). The cellular morphology of LBL-cAMP-SCAPs and LBL-SCAPs on Gel-HA was analyzed by SEM for 21 days. Cell proliferation and viability was assessed by Cell Counting Kit-8 and live/dead staining, respectively after 1 and 7 days. The osteogenic differentiation of SCAPs was evaluated by alkaline phosphatase activity (ALP) and the expression of osteogenic related genes after 21 days. Results: SEM revealed that interconnected pore architecture with HA crystals embedded within the gelatin surface. EDX confirmed the prominent element on the surface of Gel-HA was Calcium and Phosphorous. SEM revealed that both LBL-cAMP-SCAPs and LBL-SCAPs on Gel-HA exhibited spheroid morphology after 1 day, and filopodial structure after 7 days. LBL-cAMP-SCAPs formed extra cellular matrix on the surface of Gel-HA after 21 days. No significant difference in cell proliferation and viability was found between LBL-cAMP-SCAPs and LBL-SCAPs. LBL-cAMP-SCAPs seeded on the Gel-HA showed higher level of ALP activity than LBL-SCAPs. Conclusions: cAMP encapsulated by LBL self-assembly enhanced osteogenic differentiation of SCAPs on gelatin based hydroxyapatite scaffold.
DescriptionPoster Session: Dental Pulp Cells, Inflammatory and Regenerative Aspects - Presentation ID: 1553
Persistent Identifierhttp://hdl.handle.net/10722/278683

 

DC FieldValueLanguage
dc.contributor.authorZhang, JJ-
dc.contributor.authorZhang, C-
dc.contributor.authorChu, CH-
dc.date.accessioned2019-10-21T02:12:04Z-
dc.date.available2019-10-21T02:12:04Z-
dc.date.issued2019-
dc.identifier.citationThe 97th General Session of the International Association of Dental Research (IADR) held with the 48th Annual Meeting of the American Association for Dental Research (AADR) & the 43rd Annual Meeting of the Canadian Association for Dental Research (CADR), Vancouver, BC, Canada, 19-22 June 2019. In Journal of Dental Research, 2019, v. 98 n. Spec ISS A, article no. 1553-
dc.identifier.urihttp://hdl.handle.net/10722/278683-
dc.descriptionPoster Session: Dental Pulp Cells, Inflammatory and Regenerative Aspects - Presentation ID: 1553-
dc.description.abstractObjectives: To investigate osteogenic differentiation of cyclic adenosine monophosphate (cAMP) encapsulated stem cells from apical papilla (SCAPs) by layer-by-layer (LBL) self-assembly on gelatin based hydroxyapatite (HA) scaffold. Methods: Gelatin gel were alternately soaked in solution of CaCl2 and Na2HPO4 to prepare the Gel-HA scaffold. The physico-chemical characteristics of Gel-HA were analyzed by scanning electron microscopy (SEM) and energy dispersive X-ray analysis (EDX). SCAPs were encapsulated with cAMP using LBL self-assembled gelatin and alginate polyelectrolytes (LBL-cAMP-SCAPs). SCAPs loaded LBL self-assembled without cAMP was used as negative control (LBL-SCAPs). The cellular morphology of LBL-cAMP-SCAPs and LBL-SCAPs on Gel-HA was analyzed by SEM for 21 days. Cell proliferation and viability was assessed by Cell Counting Kit-8 and live/dead staining, respectively after 1 and 7 days. The osteogenic differentiation of SCAPs was evaluated by alkaline phosphatase activity (ALP) and the expression of osteogenic related genes after 21 days. Results: SEM revealed that interconnected pore architecture with HA crystals embedded within the gelatin surface. EDX confirmed the prominent element on the surface of Gel-HA was Calcium and Phosphorous. SEM revealed that both LBL-cAMP-SCAPs and LBL-SCAPs on Gel-HA exhibited spheroid morphology after 1 day, and filopodial structure after 7 days. LBL-cAMP-SCAPs formed extra cellular matrix on the surface of Gel-HA after 21 days. No significant difference in cell proliferation and viability was found between LBL-cAMP-SCAPs and LBL-SCAPs. LBL-cAMP-SCAPs seeded on the Gel-HA showed higher level of ALP activity than LBL-SCAPs. Conclusions: cAMP encapsulated by LBL self-assembly enhanced osteogenic differentiation of SCAPs on gelatin based hydroxyapatite scaffold.-
dc.languageeng-
dc.publisherInternational Association for Dental Research. The Journal's web site is located at http://www.iadr.org/-
dc.relation.ispartofJournal of Dental Research (Spec Issue)-
dc.relation.ispartofIADR/AADR/CADR 2019 General Session & Exhibition-
dc.titleEnhanced Osteogenic Differentiation by cAMP-loaded Layer-by-layer Self-assembly on Gelatin-Based Hydroxyapatite-
dc.typeConference_Paper-
dc.identifier.emailZhang, C: zhangcf@hku.hk-
dc.identifier.emailChu, CH: chchu@hku.hk-
dc.identifier.authorityZhang, C=rp01408-
dc.identifier.authorityChu, CH=rp00022-
dc.identifier.hkuros307281-
dc.identifier.volume98-
dc.identifier.issueSpec ISS A-
dc.identifier.spagearticle no. 1553-
dc.identifier.epagearticle no. 1553-
dc.publisher.placeUnited States-

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