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Article: Concurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2

TitleConcurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2
Authors
Issue Date2020
PublisherOxford University Press (OUP): Policy C - Option B. The Journal's web site is located at http://nar.oxfordjournals.org/
Citation
Nucleic Acids Research, 2020, Epub 2020-02-04 How to Cite?
AbstractSome transcription factors that specifically bind double-stranded DNA appear to also function as RNA-binding proteins. Here, we demonstrate that the transcription factor Sox2 is able to directly bind RNA in vitro as well as in mouse and human cells. Sox2 targets RNA via a 60-amino-acid RNA binding motif (RBM) positioned C-terminally of the DNA binding high mobility group (HMG) box. Sox2 can associate with RNA and DNA simultaneously to form ternary RNA/Sox2/DNA complexes. Deletion of the RBM does not affect selection of target genes but mitigates binding to pluripotency related transcripts, switches exon usage and impairs the reprogramming of somatic cells to a pluripotent state. Our findings designate Sox2 as a multi-functional factor that associates with RNA whilst binding to cognate DNA sequences, suggesting that it may co-transcriptionally regulate RNA metabolism during somatic cell reprogramming.
Persistent Identifierhttp://hdl.handle.net/10722/280343
ISSN
2017 Impact Factor: 11.561
2015 SCImago Journal Rankings: 7.458

 

DC FieldValueLanguage
dc.contributor.authorHou, LL-
dc.contributor.authorWei, Y-
dc.contributor.authorLin, YY-
dc.contributor.authorWang, X-
dc.contributor.authorLai, Y-
dc.contributor.authorYin, M-
dc.contributor.authorChen, Y-
dc.contributor.authorGuo, X-
dc.contributor.authorWu, S-
dc.contributor.authorZhu, Y-
dc.contributor.authorYuan, J-
dc.contributor.authorTariq, M-
dc.contributor.authorLi, N-
dc.contributor.authorSun, H-
dc.contributor.authorWang, H-
dc.contributor.authorZhaog, X-
dc.contributor.authorChen, J-
dc.contributor.authorBao, X-
dc.contributor.authorJauch, R-
dc.date.accessioned2020-02-07T07:39:46Z-
dc.date.available2020-02-07T07:39:46Z-
dc.date.issued2020-
dc.identifier.citationNucleic Acids Research, 2020, Epub 2020-02-04-
dc.identifier.issn0305-1048-
dc.identifier.urihttp://hdl.handle.net/10722/280343-
dc.description.abstractSome transcription factors that specifically bind double-stranded DNA appear to also function as RNA-binding proteins. Here, we demonstrate that the transcription factor Sox2 is able to directly bind RNA in vitro as well as in mouse and human cells. Sox2 targets RNA via a 60-amino-acid RNA binding motif (RBM) positioned C-terminally of the DNA binding high mobility group (HMG) box. Sox2 can associate with RNA and DNA simultaneously to form ternary RNA/Sox2/DNA complexes. Deletion of the RBM does not affect selection of target genes but mitigates binding to pluripotency related transcripts, switches exon usage and impairs the reprogramming of somatic cells to a pluripotent state. Our findings designate Sox2 as a multi-functional factor that associates with RNA whilst binding to cognate DNA sequences, suggesting that it may co-transcriptionally regulate RNA metabolism during somatic cell reprogramming.-
dc.languageeng-
dc.publisherOxford University Press (OUP): Policy C - Option B. The Journal's web site is located at http://nar.oxfordjournals.org/-
dc.relation.ispartofNucleic Acids Research-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.titleConcurrent binding to DNA and RNA facilitates the pluripotency reprogramming activity of Sox2-
dc.typeArticle-
dc.identifier.emailJauch, R: ralf@hku.hk-
dc.identifier.authorityJauch, R=rp02383-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1093/nar/gkaa067-
dc.identifier.hkuros309119-
dc.identifier.volumeEpub 2020-02-04-
dc.publisher.placeUnited Kingdom-

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