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Article: Inhibition of multidrug-resistant foodborne Staphylococcus aureus biofilms by a natural terpenoid (+)-nootkatone and related molecular mechanism

TitleInhibition of multidrug-resistant foodborne Staphylococcus aureus biofilms by a natural terpenoid (+)-nootkatone and related molecular mechanism
Authors
Keywords(+)-Nootkatone
Phytochemical
Multidrug resistance
Staphylococcus aureus
Biofilm
Issue Date2020
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/foodcont
Citation
Food Control, 2020, v. 112, p. article no. 107154 How to Cite?
AbstractStaphylococcus aureus, a foodborne pathogen, poses serious problems to the food industries owing to biofilm formation, and over 25% of the foodborne illnesses in China have been attributed to S. aureus only. Phytochemicals are widely used as anti-biofilm agents with promising efficacy, and most of them are widely available and safe. This study reported the anti-biofilm efficacy of (+)-nootkatone, a sesquiterpene ketone found in a common fruit grapefruit, against multidrug-resistant S. aureus and its potential molecular mechanism. (+)-Nootkatone exhibited bacteriostatic and bactericidal effects at 200 and 400 μg/mL, respectively, against S. aureus SJTUF 20758 and S. aureus ATCC 25923. Crystal violet staining indicated that (+)-nootkatone inhibited S. aureus biofilm formation (p < 0.05) at a sub-MIC of 50 μg/mL and reduced exopolysaccharide production. The thickness of biofilms was significantly reduced by (+)-nootkatone, which was supported by the light microscopy and confocal laser scanning microscopy. Growth curve of bacteria showed that the antibacerial activity of (+)-nootkatone was dose-dependent, and the sub-MIC concentrations did not affect the bacterial growth of planktonic cells. Besides, (+)-nootkatone affected the sliding motility of S. aureus. At 200 μg/mL, (+)-nootkatone led to the reduction of preformed biofilm mass by 50% and bacterial cell death of 79%, accompanied with a reduction of exopolysaccharide. The expression of biofilm-related genes, including sarA, icaA, agrA, RNAIII, and spa, was suppressed by (+)-nootkatone, as revealed by the transcriptional analysis. Additionally, MTT assay revealed that there was no toxicity of (+)-nootkatone to the human foreskin fibroblasts (HFF) cells. Therefore, (+)-nootkatone is a promising phytochemical against S. aureus biofilms, and has the potential to be used in food industry to fight against S. aureus-induced safety issues.
Persistent Identifierhttp://hdl.handle.net/10722/281257
ISSN
2019 Impact Factor: 4.258
2015 SCImago Journal Rankings: 1.516

 

DC FieldValueLanguage
dc.contributor.authorFARHA, AK-
dc.contributor.authorYANG, QQ-
dc.contributor.authorKIM, G-
dc.contributor.authorZHANG, D-
dc.contributor.authorMAVUMENGWANA, V-
dc.contributor.authorHabimana, O-
dc.contributor.authorLI, H-B-
dc.contributor.authorCORKE, H-
dc.contributor.authorGAN, R-Y-
dc.date.accessioned2020-03-09T09:52:11Z-
dc.date.available2020-03-09T09:52:11Z-
dc.date.issued2020-
dc.identifier.citationFood Control, 2020, v. 112, p. article no. 107154-
dc.identifier.issn0956-7135-
dc.identifier.urihttp://hdl.handle.net/10722/281257-
dc.description.abstractStaphylococcus aureus, a foodborne pathogen, poses serious problems to the food industries owing to biofilm formation, and over 25% of the foodborne illnesses in China have been attributed to S. aureus only. Phytochemicals are widely used as anti-biofilm agents with promising efficacy, and most of them are widely available and safe. This study reported the anti-biofilm efficacy of (+)-nootkatone, a sesquiterpene ketone found in a common fruit grapefruit, against multidrug-resistant S. aureus and its potential molecular mechanism. (+)-Nootkatone exhibited bacteriostatic and bactericidal effects at 200 and 400 μg/mL, respectively, against S. aureus SJTUF 20758 and S. aureus ATCC 25923. Crystal violet staining indicated that (+)-nootkatone inhibited S. aureus biofilm formation (p < 0.05) at a sub-MIC of 50 μg/mL and reduced exopolysaccharide production. The thickness of biofilms was significantly reduced by (+)-nootkatone, which was supported by the light microscopy and confocal laser scanning microscopy. Growth curve of bacteria showed that the antibacerial activity of (+)-nootkatone was dose-dependent, and the sub-MIC concentrations did not affect the bacterial growth of planktonic cells. Besides, (+)-nootkatone affected the sliding motility of S. aureus. At 200 μg/mL, (+)-nootkatone led to the reduction of preformed biofilm mass by 50% and bacterial cell death of 79%, accompanied with a reduction of exopolysaccharide. The expression of biofilm-related genes, including sarA, icaA, agrA, RNAIII, and spa, was suppressed by (+)-nootkatone, as revealed by the transcriptional analysis. Additionally, MTT assay revealed that there was no toxicity of (+)-nootkatone to the human foreskin fibroblasts (HFF) cells. Therefore, (+)-nootkatone is a promising phytochemical against S. aureus biofilms, and has the potential to be used in food industry to fight against S. aureus-induced safety issues.-
dc.languageeng-
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/foodcont-
dc.relation.ispartofFood Control-
dc.subject(+)-Nootkatone-
dc.subjectPhytochemical-
dc.subjectMultidrug resistance-
dc.subjectStaphylococcus aureus-
dc.subjectBiofilm-
dc.titleInhibition of multidrug-resistant foodborne Staphylococcus aureus biofilms by a natural terpenoid (+)-nootkatone and related molecular mechanism-
dc.typeArticle-
dc.identifier.emailHabimana, O: ohabim@hku.hk-
dc.identifier.authorityHabimana, O=rp02169-
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.foodcont.2020.107154-
dc.identifier.scopuseid_2-s2.0-85078998328-
dc.identifier.hkuros309380-
dc.identifier.volume112-
dc.identifier.spagearticle no. 107154-
dc.identifier.epagearticle no. 107154-
dc.publisher.placeUnited Kingdom-

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