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Article: Flavonoids are indispensable for complete male fertility in rice

TitleFlavonoids are indispensable for complete male fertility in rice
Authors
KeywordsChalcone synthaseflavanonesflavonesflavone C-glycosidesflavonolsmale fertilityrice (Oryza sativa)
Issue Date2020
PublisherOxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/
Citation
Journal of Experimental Botany, 2020, Epub 2020-05-29 How to Cite?
AbstractFlavonoids are essential for male fertility in some but not all plant species. In rice, the chalcone synthase mutant oschs1 produces flavonoid-depleted pollens and is male sterile. The mutant pollens are viable with normal structure but they displayed reduced germination rate and pollen tube length. Analysis of pollens of oschs1/+ heterozygous lines showed that pollen flavonoid deposition is a paternal effect and fertility is independent from the haploid genotypes (OsCHS1 or oschs1). To understand which classes of flavonoids are involved in male fertility, we further analyzed rice mutants for branch-point enzymes of the downstream flavonoid pathways, including flavanone 3-hydroxylase (OsF3H; flavonol pathway entry enzyme), flavone synthase II (CYP93G1; flavone pathway entry enzyme) and flavanone 2-hydroxylase (CYP93G2; flavone C-glycoside pathway entry enzyme). Rice osf3h and cyp93g1 cyp93g2 CRISPR/Cas9 mutants, and cyp93g1 and cyp93g2 T-DNA insertional mutants showed altered flavonoid profiles in anthers but only osf3h and cyp93g1 cyp93g2 mutants displayed reduction in seed yield. Our findings indicated that flavonoids are essential for complete male fertility in rice and a combination of different classes (flavanones, flavonols, flavones, and flavone C-glycosides) are likely to be important, as opposed to the essential role played primarily by flavonols as reported previously in several plant species.
Persistent Identifierhttp://hdl.handle.net/10722/282251
ISSN
2019 Impact Factor: 5.908
2015 SCImago Journal Rankings: 2.798

 

DC FieldValueLanguage
dc.contributor.authorWANG, L-
dc.contributor.authorLAM, PY-
dc.contributor.authorLUI, ACW-
dc.contributor.authorZhu, F-Y-
dc.contributor.authorChen, M-X-
dc.contributor.authorLiu, H-
dc.contributor.authorZhang, J-
dc.contributor.authorLo, C-
dc.date.accessioned2020-05-05T14:32:44Z-
dc.date.available2020-05-05T14:32:44Z-
dc.date.issued2020-
dc.identifier.citationJournal of Experimental Botany, 2020, Epub 2020-05-29-
dc.identifier.issn0022-0957-
dc.identifier.urihttp://hdl.handle.net/10722/282251-
dc.description.abstractFlavonoids are essential for male fertility in some but not all plant species. In rice, the chalcone synthase mutant oschs1 produces flavonoid-depleted pollens and is male sterile. The mutant pollens are viable with normal structure but they displayed reduced germination rate and pollen tube length. Analysis of pollens of oschs1/+ heterozygous lines showed that pollen flavonoid deposition is a paternal effect and fertility is independent from the haploid genotypes (OsCHS1 or oschs1). To understand which classes of flavonoids are involved in male fertility, we further analyzed rice mutants for branch-point enzymes of the downstream flavonoid pathways, including flavanone 3-hydroxylase (OsF3H; flavonol pathway entry enzyme), flavone synthase II (CYP93G1; flavone pathway entry enzyme) and flavanone 2-hydroxylase (CYP93G2; flavone C-glycoside pathway entry enzyme). Rice osf3h and cyp93g1 cyp93g2 CRISPR/Cas9 mutants, and cyp93g1 and cyp93g2 T-DNA insertional mutants showed altered flavonoid profiles in anthers but only osf3h and cyp93g1 cyp93g2 mutants displayed reduction in seed yield. Our findings indicated that flavonoids are essential for complete male fertility in rice and a combination of different classes (flavanones, flavonols, flavones, and flavone C-glycosides) are likely to be important, as opposed to the essential role played primarily by flavonols as reported previously in several plant species.-
dc.languageeng-
dc.publisherOxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/-
dc.relation.ispartofJournal of Experimental Botany-
dc.rightsPre-print: Journal Title] ©: [year] [owner as specified on the article] Published by Oxford University Press [on behalf of xxxxxx]. All rights reserved. Pre-print (Once an article is published, preprint notice should be amended to): This is an electronic version of an article published in [include the complete citation information for the final version of the Article as published in the print edition of the Journal.] Post-print: This is a pre-copy-editing, author-produced PDF of an article accepted for publication in [insert journal title] following peer review. The definitive publisher-authenticated version [insert complete citation information here] is available online at: xxxxxxx [insert URL that the author will receive upon publication here]. -
dc.subjectChalcone synthaseflavanonesflavonesflavone C-glycosidesflavonolsmale fertilityrice (Oryza sativa)-
dc.titleFlavonoids are indispensable for complete male fertility in rice-
dc.typeArticle-
dc.identifier.emailLo, C: clivelo@hku.hk-
dc.identifier.authorityLo, C=rp00751-
dc.identifier.doi10.1093/jxb/eraa204-
dc.identifier.pmid32386058-
dc.identifier.hkuros309803-
dc.identifier.volumeEpub 2020-05-29-
dc.publisher.placeUnited Kingdom-

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