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- Publisher Website: 10.1016/j.bbrc.2020.04.033
- Scopus: eid_2-s2.0-85083850126
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Article: Psoralen as an interstrand DNA crosslinker in the selection of DNA-Encoded dynamic chemical library
Title | Psoralen as an interstrand DNA crosslinker in the selection of DNA-Encoded dynamic chemical library |
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Authors | |
Keywords | DNA-Encoded chemical library Dynamic combinatorial library High throughput screening Drug discovery Psoralen |
Issue Date | 2020 |
Publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description |
Citation | Biochemical and Biophysical Research Communications, 2020, v. 533 n. 2, p. 215-222 How to Cite? |
Abstract | DNA-encoded chemical library (DEL) has emerged as a powerful technology for ligand discovery in biomedical research. Recently, we have developed a DNA-encoded dynamic library (DEDL) approach by incorporating the concept of dynamic combinatorial library (DCL) with DELs. DEDL has shown excellent potential in ligand discovery towards a variety of protein targets. However, the requirement of having a pair of unnatural p-stilbazoles as the interstrand DNA crosslinker has limited the chemical diversity of DEDLs. Here, we replaced p-stilbazole with psoralen (PS) and tested the feasibility of psoralen as the crosslinker in DEDL selection. Since psoralen is commercially available and does not require any special crosslinking partner, existing DELs may be directly used to create high-diversity DEDLs. This study is expected to greatly facilitate the development of DEDLs as a versatile tool in drug discovery. |
Persistent Identifier | http://hdl.handle.net/10722/282511 |
ISSN | 2023 Impact Factor: 2.5 2023 SCImago Journal Rankings: 0.770 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhou, Y | - |
dc.contributor.author | PENG, J | - |
dc.contributor.author | SHEN, W | - |
dc.contributor.author | Li, X | - |
dc.date.accessioned | 2020-05-15T05:29:04Z | - |
dc.date.available | 2020-05-15T05:29:04Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Biochemical and Biophysical Research Communications, 2020, v. 533 n. 2, p. 215-222 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10722/282511 | - |
dc.description.abstract | DNA-encoded chemical library (DEL) has emerged as a powerful technology for ligand discovery in biomedical research. Recently, we have developed a DNA-encoded dynamic library (DEDL) approach by incorporating the concept of dynamic combinatorial library (DCL) with DELs. DEDL has shown excellent potential in ligand discovery towards a variety of protein targets. However, the requirement of having a pair of unnatural p-stilbazoles as the interstrand DNA crosslinker has limited the chemical diversity of DEDLs. Here, we replaced p-stilbazole with psoralen (PS) and tested the feasibility of psoralen as the crosslinker in DEDL selection. Since psoralen is commercially available and does not require any special crosslinking partner, existing DELs may be directly used to create high-diversity DEDLs. This study is expected to greatly facilitate the development of DEDLs as a versatile tool in drug discovery. | - |
dc.language | eng | - |
dc.publisher | Academic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description | - |
dc.relation.ispartof | Biochemical and Biophysical Research Communications | - |
dc.subject | DNA-Encoded chemical library | - |
dc.subject | Dynamic combinatorial library | - |
dc.subject | High throughput screening | - |
dc.subject | Drug discovery | - |
dc.subject | Psoralen | - |
dc.title | Psoralen as an interstrand DNA crosslinker in the selection of DNA-Encoded dynamic chemical library | - |
dc.type | Article | - |
dc.identifier.email | Zhou, Y: yuchow@hku.hk | - |
dc.identifier.email | Li, X: xiaoyuli@hku.hk | - |
dc.identifier.authority | Li, X=rp02080 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.bbrc.2020.04.033 | - |
dc.identifier.scopus | eid_2-s2.0-85083850126 | - |
dc.identifier.hkuros | 309883 | - |
dc.identifier.volume | 533 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 215 | - |
dc.identifier.epage | 222 | - |
dc.identifier.isi | WOS:000583921700004 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0006-291X | - |