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- Publisher Website: 10.18632/aging.103090
- Scopus: eid_2-s2.0-85084270931
- PMID: 32335545
- WOS: WOS:000530887200057
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Article: Sirt1 is regulated by miR-135a and involved in DNA damage repair during mouse cellular reprogramming
Title | Sirt1 is regulated by miR-135a and involved in DNA damage repair during mouse cellular reprogramming |
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Authors | |
Keywords | mouse induced pluripotent stem cells cellular reprogramming Sirt1 miR-135a DNA damage repair |
Issue Date | 2020 |
Publisher | Impact Journals LLC. The Journal's web site is located at http://www.impactaging.com |
Citation | Aging, 2020, v. 12 n. 8, p. 7431-7447 How to Cite? |
Abstract | Sirt1 facilitates the reprogramming of mouse somatic cells into induced pluripotent stem cells (iPSCs). It is regulated by micro-RNA and reported to be a target of miR-135a. However, their relationship and roles on cellular reprogramming remain unknown. In this study, we found negative correlations between miR-135a and Sirt1 during mouse embryonic stem cells differentiation and mouse embryonic fibroblasts reprogramming. We further found that the reprogramming efficiency was reduced by the overexpression of miR-135a precursor but induced by the miR-135a inhibitor. Co-immunoprecipitation followed by mass spectrometry identified 21 SIRT1 interacting proteins including KU70 and WRN, which were highly enriched for DNA damage repair. In accordance, Sirt1 activator resveratrol reduced DNA damage during the reprogramming process. Wrn was regulated by miR-135a and resveratrol partly rescued the impaired reprogramming efficiency induced by Wrn knockdown. This study showed Sirt1, being partly regulated by miR-135a, bound proteins involved in DNA damage repair and enhanced the iPSCs production. |
Persistent Identifier | http://hdl.handle.net/10722/282542 |
ISSN | 2023 Impact Factor: 3.9 2023 SCImago Journal Rankings: 1.180 |
PubMed Central ID | |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Chen, ACH | - |
dc.contributor.author | PENG, Q | - |
dc.contributor.author | Fong, SW | - |
dc.contributor.author | Yeung, WSB | - |
dc.contributor.author | Lee, YL | - |
dc.date.accessioned | 2020-05-15T05:29:28Z | - |
dc.date.available | 2020-05-15T05:29:28Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Aging, 2020, v. 12 n. 8, p. 7431-7447 | - |
dc.identifier.issn | 1945-4589 | - |
dc.identifier.uri | http://hdl.handle.net/10722/282542 | - |
dc.description.abstract | Sirt1 facilitates the reprogramming of mouse somatic cells into induced pluripotent stem cells (iPSCs). It is regulated by micro-RNA and reported to be a target of miR-135a. However, their relationship and roles on cellular reprogramming remain unknown. In this study, we found negative correlations between miR-135a and Sirt1 during mouse embryonic stem cells differentiation and mouse embryonic fibroblasts reprogramming. We further found that the reprogramming efficiency was reduced by the overexpression of miR-135a precursor but induced by the miR-135a inhibitor. Co-immunoprecipitation followed by mass spectrometry identified 21 SIRT1 interacting proteins including KU70 and WRN, which were highly enriched for DNA damage repair. In accordance, Sirt1 activator resveratrol reduced DNA damage during the reprogramming process. Wrn was regulated by miR-135a and resveratrol partly rescued the impaired reprogramming efficiency induced by Wrn knockdown. This study showed Sirt1, being partly regulated by miR-135a, bound proteins involved in DNA damage repair and enhanced the iPSCs production. | - |
dc.language | eng | - |
dc.publisher | Impact Journals LLC. The Journal's web site is located at http://www.impactaging.com | - |
dc.relation.ispartof | Aging | - |
dc.subject | mouse induced pluripotent stem cells | - |
dc.subject | cellular reprogramming | - |
dc.subject | Sirt1 | - |
dc.subject | miR-135a | - |
dc.subject | DNA damage repair | - |
dc.title | Sirt1 is regulated by miR-135a and involved in DNA damage repair during mouse cellular reprogramming | - |
dc.type | Article | - |
dc.identifier.email | Chen, ACH: andycch0@hku.hk | - |
dc.identifier.email | Fong, SW: szewan11@hku.hk | - |
dc.identifier.email | Yeung, WSB: wsbyeung@hku.hk | - |
dc.identifier.email | Lee, YL: cherielee@hku.hk | - |
dc.identifier.authority | Yeung, WSB=rp00331 | - |
dc.identifier.authority | Lee, YL=rp00308 | - |
dc.description.nature | published_or_final_version | - |
dc.identifier.doi | 10.18632/aging.103090 | - |
dc.identifier.pmid | 32335545 | - |
dc.identifier.pmcid | PMC7202538 | - |
dc.identifier.scopus | eid_2-s2.0-85084270931 | - |
dc.identifier.hkuros | 309896 | - |
dc.identifier.volume | 12 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | 7431 | - |
dc.identifier.epage | 7447 | - |
dc.identifier.isi | WOS:000530887200057 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 1945-4589 | - |