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Article: The Differential Expression of Cytokines and Growth Factors after SMILE Compared with FS-LASIK in Rabbits

TitleThe Differential Expression of Cytokines and Growth Factors after SMILE Compared with FS-LASIK in Rabbits
Authors
KeywordsMicroscopy, Confocal
Cornea
Corneal nerves
Issue Date2020
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology & Visual Science, 2020, v. 61, p. article no. 55 How to Cite?
AbstractPurpose: To investigate the differential expression of cytokines and growth factors in the cornea and aqueous humor after small incision lenticule extraction (SMILE) compared with femtosecond LASIK (FS-LASIK) using rabbit model. Methods: Sixteen eyes of 16 rabbits in each group underwent SMILE or FS-LASIK with refractive correction of −6.00 DS/−1.00 DC. Eight additional rabbits served as controls. Pre- and 24 hours, 1 week, 1 month, and 3 months postoperatively, slit-lamp and anterior segment optical coherence tomography were performed, followed by cornea and aqueous humor collection. Apoptosis and proliferation were evaluated with TUNEL assay and Ki-67 immunostaining, respectively. The mRNA and protein expression of cytokines and growth factors was determined by RT-qPCR and Western blotting, respectively. Cytokine levels in the aqueous humor were detected with ELISA. Results: Compared with FS-LASIK, SMILE induced less apoptosis and proliferation in the cornea within 1 week postoperatively. Levels of IL-1β, TNF-α, and EGFR in the cornea were significantly increased after FS-LASIK compared with SMILE within 24 hours. Levels of IL-8 in the aqueous humor remained elevated until 1 week after FS-LASIK but not SMILE. TGF-β1 level was elevated up to 1 month after both procedures, while BFGF level was kept high within 1 month after SMILE but not FS-LASIK. Conclusions: SMILE could induce significantly less acute inflammation than FS-LASIK in the cornea and aqueous humor. The differential expression of TGF-β1 and BFGF between two procedures until 1 month might contribute to the post-SMILE delayed recovery and underline the importance of continued treatment postoperatively.
Persistent Identifierhttp://hdl.handle.net/10722/282860
ISSN
2019 Impact Factor: 3.47
2015 SCImago Journal Rankings: 2.008

 

DC FieldValueLanguage
dc.contributor.authorLiu, L-
dc.contributor.authorCheng, W-
dc.contributor.authorWu, D-
dc.contributor.authorChen, L-
dc.contributor.authorYu, S-
dc.contributor.authorZuo, T-
dc.contributor.authorZhang, L-
dc.contributor.authorYang, K-
dc.contributor.authorLi, H-
dc.contributor.authorZhang, H-
dc.contributor.authorWei, P-
dc.contributor.authorNg, ALK-
dc.contributor.authorCheng, GPM-
dc.contributor.authorWoo, VCP-
dc.contributor.authorYin, J-
dc.contributor.authorChiu, K-
dc.contributor.authorWang, Y-
dc.date.accessioned2020-06-05T06:22:23Z-
dc.date.available2020-06-05T06:22:23Z-
dc.date.issued2020-
dc.identifier.citationInvestigative Ophthalmology & Visual Science, 2020, v. 61, p. article no. 55-
dc.identifier.issn0146-0404-
dc.identifier.urihttp://hdl.handle.net/10722/282860-
dc.description.abstractPurpose: To investigate the differential expression of cytokines and growth factors in the cornea and aqueous humor after small incision lenticule extraction (SMILE) compared with femtosecond LASIK (FS-LASIK) using rabbit model. Methods: Sixteen eyes of 16 rabbits in each group underwent SMILE or FS-LASIK with refractive correction of −6.00 DS/−1.00 DC. Eight additional rabbits served as controls. Pre- and 24 hours, 1 week, 1 month, and 3 months postoperatively, slit-lamp and anterior segment optical coherence tomography were performed, followed by cornea and aqueous humor collection. Apoptosis and proliferation were evaluated with TUNEL assay and Ki-67 immunostaining, respectively. The mRNA and protein expression of cytokines and growth factors was determined by RT-qPCR and Western blotting, respectively. Cytokine levels in the aqueous humor were detected with ELISA. Results: Compared with FS-LASIK, SMILE induced less apoptosis and proliferation in the cornea within 1 week postoperatively. Levels of IL-1β, TNF-α, and EGFR in the cornea were significantly increased after FS-LASIK compared with SMILE within 24 hours. Levels of IL-8 in the aqueous humor remained elevated until 1 week after FS-LASIK but not SMILE. TGF-β1 level was elevated up to 1 month after both procedures, while BFGF level was kept high within 1 month after SMILE but not FS-LASIK. Conclusions: SMILE could induce significantly less acute inflammation than FS-LASIK in the cornea and aqueous humor. The differential expression of TGF-β1 and BFGF between two procedures until 1 month might contribute to the post-SMILE delayed recovery and underline the importance of continued treatment postoperatively.-
dc.languageeng-
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org-
dc.relation.ispartofInvestigative Ophthalmology & Visual Science-
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.-
dc.subjectMicroscopy, Confocal-
dc.subjectCornea-
dc.subjectCorneal nerves-
dc.titleThe Differential Expression of Cytokines and Growth Factors after SMILE Compared with FS-LASIK in Rabbits-
dc.typeArticle-
dc.identifier.emailChiu, K: datwai@hku.hk-
dc.identifier.authorityChiu, K=rp01973-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1167/iovs.61.5.55-
dc.identifier.pmid32460319-
dc.identifier.scopuseid_2-s2.0-85085540150-
dc.identifier.hkuros310199-
dc.identifier.hkuros310198-
dc.identifier.volume61-
dc.identifier.spagearticle no. 55-
dc.identifier.epagearticle no. 55-
dc.publisher.placeUnited States-

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