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- Publisher Website: 10.1021/acs.biochem.9b00787
- Scopus: eid_2-s2.0-85074882773
- PMID: 31657212
- WOS: WOS:000509420600010
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Article: OPA-Based Bifunctional Linker for Protein Labeling and Profiling
Title | OPA-Based Bifunctional Linker for Protein Labeling and Profiling |
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Authors | |
Keywords | Amino acids Chemical modification Hydrocarbons Available lysines Bi-functional |
Issue Date | 2020 |
Publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/biochemistry |
Citation | Biochemistry, 2020, v. 59 n. 2, p. 175-178 How to Cite? |
Abstract | Lysine residues have been considered as a routine conjugating site for protein chemical labeling and modification. The commercially available lysine-labeling agents have several limitations in labeling efficiency, stability, and cost. To pursue alternative protein lysine-labeling strategies, herein, we report the development of an ortho-phthalaldehyde (OPA)-based bifunctional linker suitable for protein chemical labeling and profiling. Among three designed OPA-based bifunctional linkers, OPA-NH-alkyne 5 was proved to be optimal for protein labeling with minimal protein turbidity. We further demonstrated OPA-NH-alkyne 5 was applicable for immediate capture of protein or proteome chemical labeling. |
Persistent Identifier | http://hdl.handle.net/10722/284792 |
ISSN | 2023 Impact Factor: 2.9 2023 SCImago Journal Rankings: 1.042 |
ISI Accession Number ID |
DC Field | Value | Language |
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dc.contributor.author | Zhang, Q | - |
dc.contributor.author | ZHANG, Y | - |
dc.contributor.author | LIU, H | - |
dc.contributor.author | Chow, HY | - |
dc.contributor.author | Tian, R | - |
dc.contributor.author | Fung, YME | - |
dc.contributor.author | Li, XC | - |
dc.date.accessioned | 2020-08-07T09:02:41Z | - |
dc.date.available | 2020-08-07T09:02:41Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Biochemistry, 2020, v. 59 n. 2, p. 175-178 | - |
dc.identifier.issn | 0006-2960 | - |
dc.identifier.uri | http://hdl.handle.net/10722/284792 | - |
dc.description.abstract | Lysine residues have been considered as a routine conjugating site for protein chemical labeling and modification. The commercially available lysine-labeling agents have several limitations in labeling efficiency, stability, and cost. To pursue alternative protein lysine-labeling strategies, herein, we report the development of an ortho-phthalaldehyde (OPA)-based bifunctional linker suitable for protein chemical labeling and profiling. Among three designed OPA-based bifunctional linkers, OPA-NH-alkyne 5 was proved to be optimal for protein labeling with minimal protein turbidity. We further demonstrated OPA-NH-alkyne 5 was applicable for immediate capture of protein or proteome chemical labeling. | - |
dc.language | eng | - |
dc.publisher | American Chemical Society. The Journal's web site is located at http://pubs.acs.org/biochemistry | - |
dc.relation.ispartof | Biochemistry | - |
dc.rights | This document is the Accepted Manuscript version of a Published Work that appeared in final form in [JournalTitle], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see http://pubs.acs.org/page/policy/articlesonrequest/index.html]. | - |
dc.subject | Amino acids | - |
dc.subject | Chemical modification | - |
dc.subject | Hydrocarbons | - |
dc.subject | Available lysines | - |
dc.subject | Bi-functional | - |
dc.title | OPA-Based Bifunctional Linker for Protein Labeling and Profiling | - |
dc.type | Article | - |
dc.identifier.email | Zhang, Q: qingz@hku.hk | - |
dc.identifier.email | Chow, HY: hchowhy@connect.hku.hk | - |
dc.identifier.email | Fung, YME: eva.fungym@hku.hk | - |
dc.identifier.email | Li, XC: xuechenl@hku.hk | - |
dc.identifier.authority | Zhang, Q=rp02542 | - |
dc.identifier.authority | Fung, YME=rp01986 | - |
dc.identifier.authority | Li, XC=rp00742 | - |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1021/acs.biochem.9b00787 | - |
dc.identifier.pmid | 31657212 | - |
dc.identifier.scopus | eid_2-s2.0-85074882773 | - |
dc.identifier.hkuros | 311893 | - |
dc.identifier.volume | 59 | - |
dc.identifier.issue | 2 | - |
dc.identifier.spage | 175 | - |
dc.identifier.epage | 178 | - |
dc.identifier.isi | WOS:000509420600010 | - |
dc.publisher.place | United States | - |
dc.identifier.issnl | 0006-2960 | - |